Abstract:

〕Objective: To study the effect of extracts form Mirabilis jalapa on the damaged L-02 human embryo hepatocytes induced
by ethanol. Methods: L- 02 human embryo hepatocytes in logarithmic phase were chosen and damaged by ethanol at a final
concentration of 100 mmol/L. After 8 hours, different concentrations of the extracts form Mirabilis jalapa were added in. After 16 hours
of cultivation, MTT method was used to observe inhibition rate of L-02 human embryo hepatocytes damaged by ethanol, and Elisa
method was used to detect the activity of glutamic- pyruvic transaminase（ALT）, glutamic- oxalacetic transaminase（AST） and
superoxide dismutase（SOD）, and the content of malonaldehyde（MDA）in nutrient solution. Results: 8 mg/mL or higher crude herbal
concentrations of Mirabilis jalapa can significantly decrease inhibition rate of ethanol-induced damaged L-02 cells, obviously reduce
ALT and AST activity, increase SOD activity and decrease MDA content in the culture for damaged cells. Conclusion: Extracts form
Mirabilis jalapa has protective effect on the damaged L-02 cells induced by ethanol.

Key words: Mirabilis jalapa, ethanol, L-02 human embryo hepatocytes, protective effect