Difference Analysis of Free Anthraquinones, Combined Anthraquinones and Total Anthraquinones in
Cultivated Rheum palmatum L. in Different Producing Areas

Abstract

Abstract:

Objective: To determine content of free anthraquinones, combined anthraquinones and total anthraquinones in cultivated
Rheum palmatum L. in different producing areas, and to provide scientific basis for quality assessment and reasonable using. Methods:
Agela Venusil XBP-C18 column（4.6 mm×250 mm, 5 μm）was used with the mobile phase of methanol-0.1% posphoric acid solution
（75：25）at the flow rate of 1.0 mL/min, the detection wavelength was set at 254 nm and the column temperature was 35 ℃. To
determine the content of cultivated Rheum palmatum L. from 11 different producing areas, cluster analysis was carried out for the
results. Results: 5 kinds of anthraquinons exist in cultivated Rheum palmatum L. from different producing areas, in which cultivated
Rheum palmatum L. from Lixian, Gangsu was the best. Cluster analysis divided cultivated Rheum palmatum L. from 11 different
producing areas into 3 categories. Conclusion: This paper analyzes the quality of cultivated Rheum palmatum L. from different
producing areas, it could provide reference for quality assessment and standardized planting of cultivated Rheum palmatum L. in Dali.

Key words: Rheum palmatum L., different producing areas, anthraquinons, HLPC, determination, cluster analysis

CLC Number:

R917

Yang Min, Pan Xingjiao, Jiang Jing, Yao Yuting, Zhao Yang, Zhou Nong. Difference Analysis of Free Anthraquinones, Combined Anthraquinones and Total Anthraquinones in
Cultivated Rheum palmatum L. in Different Producing Areas[J]. J4, 2016, 1(10): 9-13.

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