Abstract:

Objective: To study the sodium nitrite injury and repair mechanisms to the male mice. Methods: Kunming species male
mice were used in the experiment. Dose groups were administered by oral with NaNO2（15, 30, 60 mg/（kg·d））, while the control
groups had been given normal saline of the same volume for 14 days. After 24 hours of the last exposure to NaNO2, one-third of mice in
three dose groups were killed. The remaining mice were divided into two groups. One group was given Lyceum barbarum
polysaccharide, while another group was given normal saline of the same volume for 14 days and then killed all the mice. Experiment
measured index included the main organ weight, organ coefficient, serum testosterone level and sperm deformity rate. Results: With
the increasing dose of NaNO2, the mice serum testosterone level and the spleen weight were decreased significantly（all P value＜
0.05）, the sperm deformity rate was increased significantly（all P value＜0.05）. After stopping giving NaNO2, the level of serum
testosterone were increased, the sperm deformity rate and spleen organ coefficient were declined in 15, 30 mg/（kg·d）NaNO2 groups,
the three indexes were statistically significant comparing with previous experiments（all P value＜0.05）. The other indexes had no
change. Conclusion: NaNO2 had the ability to inhibit the level of serum testosterone and induce the sperm deformity in mice. After
stopping exposing to NaNO2, the mice can repair some damages by themselves, the Lyceum barbarum polysaccharide may also have
recovery effect, which needs further study.

Key words: NaNO2, organ coefficient, hormone level, sperm deformity