J4 ›› 2016, Vol. 1 ›› Issue (10): 30-34.

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The Correlation between Point Mutation of T3394C in Mitochondrial ND1 Gene and Patients with Type 2
Diabetes Mellitus in Aged Bai Minorities in Dali

  

  1. (1. Pre-clinical College, Dali University, Dali, Yunnan 671000, China; 2. Yunnan Provincial Key Laboratory of Entomological
    Biopharmaceutical R&D, Dali, Yunnan 671000, China; 3.Respiratory Department, Dali Teaching hospital of Dali University, Dali,
    Yunnan 671000, China)
  • Received:2015-12-09 Revised:2016-02-29 Online:2016-10-15 Published:2016-10-15

Abstract:

Objective: To investigate the correlation between point mutation of T3394C in mitochondrial NADH ND1 gene and
patients with type 2 diabetes mellitus in aged Bai minorities in Dali. Methods: 200 unrelated individuals with type 2 diabetes mellitus
(T2DM group)which were randomly chosen among aged Bai minorities in Dali, Yunnan, and 218 aged cases with normal glucose
tolerance without DM family history(Control group) were inspected. Polymerase chain reaction and restriction fragment length
polymorphism(PCR-RFLP)technology were used for screening. Results: The point mutation T3394C in mitochondrial ND1 gene
were found in 5 cases in T2DM group(2.50%), while only 1 case in control group(0.46%), and there were significant differences
between two groups(P<0.05). Mitochondrial ND1 gene T3394C point mutation in patients with type 2 diabetes mellitus showed
obvious maternal inheritance characteristics. Conclusions: The point mutation of T3394C in the mitochondrial DNA ND1 gene may be
related to the occurrence of T2DM in aged Bai minorities in Dali.

Key words: mitochondrial ND1 gene, type 2 diabetes mellitus, T3394C mutation, Bai minority

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