›› 2017, Vol. 2 ›› Issue (2): 11-19.

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Research on Anti-tumor Activity of Iron-fluorouracil Complex

  

  1. (1. Pharmaceutical Science, Kunming Medical University, Kunming 650500, China; 2. College of Chemistry, Yunnan Open University,
    Kunming 650223, China; 3 School of Basic Medicine, Kunming Medical University, Kunming 650500, China; 4. Department of
    Chemistry, Kunming University, Kunming 650214, China)
  • Received:2016-04-06 Revised:2016-06-14 Online:2017-02-15 Published:2017-02-15

Abstract:

Objective: To explore iron- fluorouracil complex's anti- tumor activity and effect to human gastric carcinoma cells
apoptosis. Methods: MTT method was used to detect inhibition rate of the complex on tumor cell lines: K562, HCT-116, SGC-7901,
MCF-7 and HEPG-2; Hoechst 33342/PI and flow cytometry(FCM)methods were used to exhibit apoptosis of SGC-7901 cells; RTqPCR
was used to measure the mRNA expression of apoptosis related factors: Caspase-3, Bax and Bcl-2. Results: Iron-fluorouracil
complex obviously inhibited the proliferation of 5 tumor cell lines. IC50 were 7.8×10-5, 5.4×10-5, 3.5×10-5, 1.1×10-4 and 2.2×10-5 mol/L,
respectively. After 36 hours' treatment on SGC-7901 cell with a concentration 10- 5 mol/L, its apoptosis rate was 9.8%, which could
promote SGC-7901 cell apoptosis than its ligands 5-Fu and Phen(P<0.01); the mRNA expression of Caspase-3 gene was up-regulated
to 4.9(P<0.01), while Bcl-2 was down-regulated to 0.2(P<0.01). Conclusion: The complex showed good anti-tumor activity in
vitro, up regulation of Caspase-3 as well as down regulation Bcl-2 may be one of its mechanisms of action.

Key words: iron-fluorouracil complex, cytotoxicity, apoptosis

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