Construction and Identification of Eukaryotic Expression Vector of Human MTERF3

Abstract

Abstract:

Objective：To construct and identify the eukaryotic expression vector of FLAG-tagged human MTERF3 gene. Methods：
The human MTERF3 gene was amplified by Polymerase Chain Reaction（PCR）. The recombinant products of the p3×FLAG-CMVhMTERF3
were gained by T4 DNA ligase connecting MTERF3 gene fragment and eukaryotic expression vector p3×FLAG-CMV-14
with restriction enzymes Hind Ⅲ and BamHⅠ. The recombinant pasmid p3×FLAG-CMV-hMTERF3 were identified by colony PCR,
double restriction enzyme digest and DNA sequencing. Results：A specific band of 1 254 bp was detected from recombinant plasmid
p3×FLAG-CMV-hMTERF3 by digestion of Hind Ⅲ and BamHⅠ. The sequencing and identification showed that homology between
this sequence and the human MTERF3 gene sequence from GenBank was 100%, and the size and the direction of the inserted gene
were right. Conclusion：The eukaryotic expression vector of human MTERF3 was constructed successfully, which may lay the
foundation for a further study of the interaction mechanism between behavior of MTERF3 in tumor cells and its development.

Key words: human MTERF3, eukaryotic expression vector, identification

CLC Number:

Zhang Xiaocan, Duan Baozhong, Luo Dandan, Tao Aien, Li Yang. Construction and Identification of Eukaryotic Expression Vector of Human MTERF3[J]. , 2017, 2(8): 18-22.

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