Table of Content

15 August 2017, Volume 2 Issue 8

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The Effects of Periplaneta americana Extract on Spleen Lymphocytes of H22 Tumor-bearing Mice

Wu Daoxun, Shao Weili, Chang Xu, Ou Hongli, Zhang Handan, Chen Junya

2017, 2(8):  1-5. 

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Objective：To study the effects of Periplanet americana extract on spleen lymphocytes of H22 Tumor- bearing mice.
Methods：BALB/c mice were administered with low dose, middle dose and high dose of Periplaneta americana extract（50,100,200
mg/kg）by gavage. The positive group was injected with 50 mg/kg cyclophosphamide. After 10-day feeding and injection respectively the
content of T lymphocytes in the spleen, lymphocyte cells, Monocyte cells and granulocyte cells in peripheral blood were measured, and
the anti-tumor rate was calculated. Results：The Periplaneta americana extract could obviously increase the content of CD8+ and the
tumor growth inhibition rate（P<0.05）compared with the normal group. Conclusion：Periplaneta americana extract can inhibit the
growth of H22 liver tumor in mice and its mechanism may be related to the increase of the content of CD8+ in the spleen.

Study on in Vitro Antibacterial Activity of Rubus delavayi

Liu Xiaobo, Guo Meixian, Shi Guirong, Guo Yuqin

2017, 2(8):  6-9. 

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Objective：To investigate in vitro antibacterial activity of the alcohol extracts and extract fractions of Rubus delavayi.
Methods：5 extract fractions were obtained through 95% ethanol reflux by extracting Rubus delavayi. The minimum inhibitory
concentration（MIC）, the minimum bactericidal concentration（MBC）and the inhibition zone of the crude extract and 5 extract
fractions were determined by tube dilution and disk diffusion method. The antibacterial activity of Rubus delavayi was observed.
Results：The alcohol extracts, its ethyl acetate fraction and its N-butanol fraction of Rubus delavayi showed high antibacterial activity
against eight tested bacteria; lower antioxidant activity was observed in the water fraction; the chloroform fraction had a significantly
inhibitory effect on Staphylococcus aureus, Proteusbacillus species, Bacillus subtilis and Salmonella typhi; the petroleum ether fraction
had a certain inhibition on Staphylococcus aureus and Bacillus subtilis. Conclusion: The alcohol extracts and its different extract
fractions of Rubus delavayi have in vitro antibacterial activity against almost all the studied bacteria.

Exploring the Contractile Effect of Geranium strictipes R. Knuth on the Intestinal Muscle of the Isolated
Guinea-pig Ileum and Its Mechanism

Geng Ling, Li Hongwen, Chen Junya

2017, 2(8):  10-12. 

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Objective：To observe the effect of ethyl acetate extracts from Geranium strictipes R. Knuth on the isolated guinea-pig
ileum smooth muscle, and to explore the mechanism of its in vitro action. Methods：The normal contraction curve was observed after
the isolated ileum specimens were prepared. Then contraction in smooth muscle caused by Geranium strictipes R. Knuth and three
blockers was recorded. Results：The Geranium strictipes R. Knuth in different doses could accelerate the ileum smooth muscle
contraction of the guinea-pig. The maximum tension, frequency and amplitude induced by 20 and 40 mg/mL extracts had a statistical
difference（P < 0.01）compared with the normal group; and it could resist against ileum smooth muscle relaxation induced by Atropine
and Chlorobenzene. Conclusion：The Geranium strictipes R. Knuth has stimulating effects on ileum smooth muscle extraction of the
guinea-pig, and the contractile effect may be related to M receptor and H1 receptor.

Effect of Different Altitudes on the Content of Iridoid Glycoside in Gentiana crassicaulis

Zhang Xiaocan, Duan Baozhong, Luo Dandan, Tao Aien, Li Yang

2017, 2(8):  13-17. 

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Objective：To observe the content of iridoid glycoside in Gentiana crassicauls growing at different altitudes and to provide
a scientific basis for the cultivation of this herb and its suitable planting areas. Methods：A HPLC method was used to measure the
content of four active components in Gentiana crassicauls at different altitudes, and chemometric methods were applied to analyze
HPLC fingerprinting and chemical recognition. Results：The content of the active components in this medical plant from 26 product
batches was in compliance with relevant standards of quality. The similarity of chromatographic fingerprints was greater than 0.9. The
experimental results indicated that the influence of altitude variations on the content of iridoid glycoside was not significant.
Conclusion：The iridoid glycoside content in the Gentiana crassicauls roots at the altitude of 2 700 m is higher than that at other
altitudes which is suitable for the cultivation of Gentiana crassicauls ．

Construction and Identification of Eukaryotic Expression Vector of Human MTERF3

Zhang Xiaocan, Duan Baozhong, Luo Dandan, Tao Aien, Li Yang

2017, 2(8):  18-22. 

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Objective：To construct and identify the eukaryotic expression vector of FLAG-tagged human MTERF3 gene. Methods：
The human MTERF3 gene was amplified by Polymerase Chain Reaction（PCR）. The recombinant products of the p3×FLAG-CMVhMTERF3
were gained by T4 DNA ligase connecting MTERF3 gene fragment and eukaryotic expression vector p3×FLAG-CMV-14
with restriction enzymes Hind Ⅲ and BamHⅠ. The recombinant pasmid p3×FLAG-CMV-hMTERF3 were identified by colony PCR,
double restriction enzyme digest and DNA sequencing. Results：A specific band of 1 254 bp was detected from recombinant plasmid
p3×FLAG-CMV-hMTERF3 by digestion of Hind Ⅲ and BamHⅠ. The sequencing and identification showed that homology between
this sequence and the human MTERF3 gene sequence from GenBank was 100%, and the size and the direction of the inserted gene
were right. Conclusion：The eukaryotic expression vector of human MTERF3 was constructed successfully, which may lay the
foundation for a further study of the interaction mechanism between behavior of MTERF3 in tumor cells and its development.

The Relationship between Endothelial Nitric Oxide Synthase rs1799983 Gene Polymorphism and
Blood Pressure

Liu Yang, Zhang Qingtao, Meng Xiangzhi, Hu Naiqing, Deng Quanliang, Li Lihua

2017, 2(8):  23-27. 

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Objective：To explore the relationship between endothelial nitric oxide synthase（eNOS）rs1799983 polymorphism and
blood pressure. Methods：All employees including the retired of a factory in Dali were enrolled in the study. A standardized
questionnaire was distributed to collect information on alcohol consumption, smoking habits and use of medications. And then the
genotypes were categorized by SNaPshot method. Results：The frequencies of rs1799983 GG, GT and TT were 80.3%, 19.0% and
0.7% respectively. There was no significant difference in genotypes and alleles between participants with normal blood pressure and
hypertensive patients（P≥0.21）. However, T allele carriers（GT + TT）compared with GG homozygotes had a significantly higher
systolic and pulse pressure both before and after adjusted for covariates（P≤0.04）. It was found that significant interaction between
rs1799983 polymorphism and low- density lipoprotein cholesterol（LDL- C）had an effect on pulse pressure（Pint = 0.04）. Pulse
pressure of T allele carrier was significantly higher than GG homozygotes as LDL- C levels increased. Conclusion：The eNOS
rs1799983 polymorphism may be associated with blood pressure and affected by LDL-C levels.

Associations of IFN-γ Gene Polymorphism with Dilated Cardiomyopathy

Zhou Li, Chen Haiyan, Kong Shan, Cheng Jiamao

2017, 2(8):  28-30. 

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Objective：To investigate the relationship between mononucleotide's polymorphism of IFN-γ gene's 874 site and the
susceptibility to dilated cardiomyopathy. Methods：In this case-control study, we have collected venous blood of 113 patients with
DCM（patients group）and of 100 healthy Han adults（control group）from the first affiliated hospital of Dali University. PCR-SSP
technology and agarose gel electrophoresis were applied to detect mononucleotide's IFN-γ +874T/A polymorphism of the two groups.
Results：There were significant differences between the patient group whose IFN-γ +874TT genotypic frequency distribution was
30.97% and the health control group with 8.00%（χ2=17.631，P<0.001）. The distributive frequencies of T allele in the patient group
and control group were 40.27% and 22.00% respectively. There were statistically significant differences between them（χ2=16.352，P<
0.001）. Conclusion：There may be a close relationship between IFN-γ +874A/T polymorphism and the susceptibility to DCM.

A Comparative Study between Laparoscopic Radical Resection and Conventional Open Surgery for
Rectal Cancer

Shao Yu, Shu Wuying, Li Meng, Tan Yunbo

2017, 2(8):  31-34. 

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Objective：To evaluate the advantages and disadvantages of laparoscopic surgery for colorectal cancer. Methods：
Clinical records of 45 patients of laparoscopic colorectal surgery from October 2010 to October 2013 were reviewed and compared with
those of 53 comparable patients on whom conventional open surgery was performed during the same period. Results：Intraoperative
blood loss, the time of recovery of bowel function and infection rate in laparoscopic group were significantly lower than those of the
control group with traditional open surgery（P<0.05）. No significant differences were detected between two groups in the rate of
anastomotic leak, anastomotic bleeding and postoperative bleeding（P>0.05）. There were no significant differences between two groups
in recurrence rate within 2 or 3 years after surgery（P>0.05）. Conclusion：Laparoscopic surgery has the same effects as traditional
radical operation for colorectal cancer, and it is a safe and feasible technique with less surgical trauma and quicker recovery.

An Analysis of the Reason of Dislocation of the Joint after the Primary Total Hip Arthroplasty

Zhao Chang, Li Hong, Hu Zhifu, Sun Zhe, Sun Chaojun

2017, 2(8):  35-40. 

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Objective：To investigate the causes of dislocation of the joint after the first total hip replacement. Methods：A
retrospective review and analysis was performed of the medical records of 432 patients（469 hips）who had the primary total hip
replacement in the first afflicted hospital of Dali University from January 2011 to December 2015 in order to explore the causes of
dislocation of the joint after the first replacement. Results：Of the 432 patients（469 hips）, 17（17 hips）had postoperative dislocation,
including 14 cases of early dislocation in which 3 cases（21.4%）were caused by patients, 6 cases（42.9%）by the operation and 5
cases（35.7%）by improper position of patients, and 3 cases of late dislocation in which 2 cases were traumatic dislocation and 1 case
of unknown reason. Conclusion：Dislocation after the first total hip replacement can be caused by patients, surgery and postoperative
factors among which the surgical factor is the main cause of early dislocation.

Short-term Efficacy of Percutaneous Transforaminal Endoscopic Discectomy in the Treatment of the
Single-segment Lumbar Disk Herniation

Yang Duming, Zhang Jiyuan, Xu Sanxiong, Zhang Shu

2017, 2(8):  41-43. 

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Objective：To evaluate the short-term efficacy of percutaneous transforaminal endoscopic discectomy in the treatment of
lumbar disk herniation. Methods：This study selected randomly 30 patients with single-segment lumbar disk herniation on whom
percutaneous transforaminal endoscopic nucleus pulposus discectomy was conducted from February 2015 to June 2016. The clinical
efficacy of treatment was evaluated by using the pain visual analog scale（VAS score）and the criteria of the improved Macnab.
Results：All the surgeries on the 30 patients were successfully completed with the operation time of 60-120 min and blood loss of 30-
60 mL. The postoperative VAS score within one day, one week and three months after surgery was significantly lower than the
preoperative one. The comparative result（P＜0.01） showed significantly statistical differences. According to the criteria of the
improved Macnab evaluation, the excellence rate was much higher with excellent in 23 cases and good in 4 cases, and the rate of
excellent and good was 90%. There were no complications such as dural rupture, nerve root injury, infection and other complications in
all patients, and MRI examination showed that the protrusion of intervertebral discs basically disappeared. Conclusion：Percutaneous
lumbar disc herniation is a safe and effective way to treat the patients with lumbar disc herniation with less trauma, less blood loss,
faster rehabilitation, shorter postoperative hospital stay and other advantages alike.

The Expression of Adipokines Vaspin and Chemerin in Pregnant Women with Gestational Diabetes
Mellitus

Li Xiaohong, Chen Zhuo, Ma Runmei

2017, 2(8):  44-48. 

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Objective：To explore the relationships between the expression of visceral adipose tissue- derived serine protease
inhibitor（vaspin）and Chemerin in serum and visceral omental adipose tissue of pregnant women with gestational diabetes mellitus.
Methods：The levels of Vaspin and Chemerin in serum were measured by enzyme-linked immuno sorbent assay（ELISA）. Fasting
plasma glucose（FPG）, fasting insulin（FINS）, total cholesterol（TC）, triglyceride（TG）and c-reactive protein（CRP）were also
detected. The expression of Vaspin and Chemerin in visceral adipose tissue was detected by western blot. Results：Pregnant women
with GDM had higher insulin levels and insulin resistant（IR）than the healthy control group. Serum Vaspin and Chemerin levels were
found to be significantly increased in patients with GDM compared with healthy control subjects. Serum Chemerin levels were
positively associated with TC, TG and CRP, while Vaspin levels in serum were positively associated with body weight before pregnancy
and BMI at delivery, HDL, FINS and HOMA-IR. The expression levels of Chemerin in obese pregnant women obviously increased.
Conclusion: Vaspin and Chemerin are closely related to GDM risk factors, obesity and lipid metabolism disorders in pregnant women.

A Comparative Analysis of Efficacy of Titanium Ear Bone and Autologous Bone Used in the Surgery to
Restore Hearing

Tang Jianwen, Hu Jinmei, Wu Chonghua

2017, 2(8):  49-52. 

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Objective：To compare the improvements of hearing function in tympanoplasty with titanium artificial ear bone and with
autologous bone. Methods: The study was first a retrospective review of the medical records of 71 patients during the period from April
2013 to April 2015, of whom 31 patients underwent tympanoplasty with titanium artificial ear bone while 40 patients with autologous
bone. Then we compare the air conduction hearing threshold and the air-bone gap（ABG）in the pre-operation and within six months
after the surgery. Results：There were significant differences in 31 cases with titanium artificial bones in which 0.5-4.0 kHz average
air conduction hearing thresholds were（56.35 ± 11.56）dB pre-operatively and（37.07 ± 10.13）dB post-operatively, and ABG were
（35.13 ± 9.13）dB pre-operatively and（15.85±4.12）dB post-operatively（P<0.05）. Significant differences could also be detected in
the other 40 patients with autologous bones who had 0.5-4.0 kHz average air conduction hearing thresholds（58.16 ±13.15）dB preoperatively
and（43.48 ± 13.03）dB post-operatively, and air-bone gaps（30.83 ± 8.88）dB pre-operatively and（18.96 ± 5.41）dB
post-operatively（P<0.05）. Mean ABG of the two groups were narrowed to 19.28 dB and 11.87 dB post-operatively, respectively.
Comparison showed that the group with titanium artificial bones had a better improvement in hearing function post-operatively than the
group with autogenous bones. There were significant difference in the comparison（P<0.05）. Conclusion：In the middle ear surgery
titanium artificial bone is an ideal prosthesis in ossicular chain reconstruction which can help restore and improve the patient's hearing
function much better.

Effects of Wang-bi Detoxification Herbal Medication on T-lymphocyte Subgroups of Patients with
Rheumatoid Arthritis

Tang Zhenli

2017, 2(8):  53-55. 

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Objective：To observe effects of Wang-bi detoxification herbal medication on T-lymphocyte subgroups of patients with
Rheumatoid Arthritis, and to explore the mechanism of it efficacy. Methods：A comparative study was conducted among 200 patients
including inpatients and outpatients who were divided into two groups randomly. 100 patients in the control group took Indometacin
Enteric-coated Tablets with 25 mg every time and once a day, and Methotrexate Tablets with 7.5 mg every time and once a week, while
100 patients in the treatment group took Wang-bi detoxification herbal medication with 200 mL each time and twice a day. After two
courses of eight weeks, we compared CD4 + and CD8 + of the two groups before and after the treatment. Results：There was no
significant difference with regard to the comparison of CD4+ and CD8+ of each group before the treatment（P＞0.05）. However, after
the treatment both of the groups had a lower CD4+ and a higher CD8 and a lower ratio of CD4+ to CD8+. Comparison between the two
groups also showed no significant difference（P＞0.05）. Conclusion：Patients with RA has an autoimmune disorder. Wang- bi
detoxification herbal medication can help the distribution of their T-lymphocyte subsets and help them keep a stable immune system,
and can have a similar immunosuppressive effect as that produced by the therapy of MTX in combination with other medicines.

The Value of ANCA in the Detection of Systemic Lupus Erythematosus Disease Activity

Zhang Xu, Qin Hongqun, Ma Shungao

2017, 2(8):  56-58. 

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Objective：To study the value of ANCA in the detection of systemic lupus erythematosus disease activity. Methods：The
serum of 53 patients was tested by indirect immunofluorescence（IIF）, and then the specific target antigen protein 3（PR3）and
myeloperoxidase（MPO）in sera of patients with ANCA were detected by enzyme-linked immunosorbent assay（ELASA）. Laboratory
indexes related to the disease activity of SLE patients were detected. Results：ANCA were detected in 21 patients with SLE: the
positive rate was 39.6%, 20 had pANCA and one Cytoplasm antibody（cANCA）. Target antigen was neither MPO nor PR3. Compared
with ANCA negative group, there was a statistically significant difference in regard to the SLE disease activity in ANCA positive group
（P<0.01）. And the anti- dsDNA antibody was positive, complement（C3, C4） decreased, ESR increased and renal injury was
significantly higher in the positive group than in the negative group. So the result was statistically significant（P<0.05）. Conclusion：
ANCA positive in the activity of SLE disease has a certain value and can be used as one of the indicators to determine the activity of
the disease.

A Multivariate Analysis of Physical Characteristics of Ethnic Minorities in Yunnan

Zhou Hanyu, Shen Yuanying, Xiao Wen

2017, 2(8):  59-65. 

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Objective：To survey the physical characteristics of nineteen ethnic minorities and the Han ethnicity in Yunnan; to
discuss the relationships and differences in physical characteristics among the chosen groups. Methods：By the way of literary review
this study combined the cluster analysis and factor analysis together to analyze the physical characteristics of 19 minorities and Han
ethnicity in Yunnan. Results：Minorities in Yunnan can be assembled into three categories in terms of their different physiques: the
one originating from Di and Qiang minorities, the one living in well-located and easy-accessing areas, and the one consisting of
aboriginals or those inhabiting in severely-isolated regions. The structure of constitutional factor is similar to the result of cluster
analysis. Demographic factors and geographic factors have certain accordance with the results of cluster analysis. Conclusion：
Nationality origin is still an important factor which influences the physical characteristics of minorities in Yunnan. Because of the
different population, administrative region and the traffic in history the physical characteristics of minorities in Yunnan are also
different. However, those living in the areas connected by roads almost have the similar physical characteristics.

An Analysis of the Epidemic Situation of Legal Infectious Diseases in Binchuan County from 2004 to 2013

Zhang Keqin, Zhang Kezhi

2017, 2(8):  66-70. 

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Objective：To understand the epidemic characteristics of notifiable infectious diseases in Binchuan County from 2004 to
2013, and to provide scientific basis for the prevention and control measures. Methods：Descriptive epidemiological method was used
to analyze the notifiable infectious diseases in Binchuan County from 2004 to 2013. Results：From 2004 to 2013 in Binchuan County
it was reported that there were 9 153 cases with 24 kinds of infectious diseases in which there were 96 deaths of B class with 76 cases
of AIDS included, and the mortality rate was 1.05%. No class A infectious diseases were reported. The average annual incidence rate
was 271.14/10 million, including 2 939 cases with pulmonary tuberculosis, the main diseases of foot and mouth disease in 2 025 cases,
1 427 cases with hepatitis, 704 cases with epidemic parotitis, 404 cases with infectious diarrhea, 343 cases with dysentery, 282 cases
with gonorrhea and 274 cases with scarlet fever. There were 8 398 cases in total, accounting for 91.75%. 16 cases of class B were
reported with an average annual incidence rate of 175.49/10 million, and 8 cases of class C with no mortality had an average annual
incidence rate of 95.65/10 million. The overall incidence rate showed an upward trend, and the class B was relatively stable.
Conclusion：The prevention and control of infectious diseases in Binchuan County is still arduous, and we should strengthen the
prevention and control of tuberculosis and hand foot mouth disease.

An Analysis of the Detection Results of Microbial Contamination in Drinking Water in Dali
from 2010 to 2013

Yang Hui, Yang Cairong, Wu Chengzu, Liu Jizheng, Li Ze

2017, 2(8):  71-74. 

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Objective：To investigate the microbial contamination of drinking water in Dali from 2010 to 2013, to provide scientific
basis for the surveillance and management of drinking water. Methods：Water samples were collected in dry season and rainy season
twice a year from 15 representative jurisdiction monitoring sites of Dali which were randomly selected. The samples were collected and
tested according to GB/T 5750—2006, and sanitary microbiology evaluation followed GB 5749—2006. Results：Over the period from
2010 to 2013 1 996 water samples were collected and tested. The differences among three types of water test dimension were statistically
significant（χ2=11.999, P<0.05）. Over the four years failure rate（43.76%） in rainy season was significantly higher than that
（11.64%）in dry season（χ2=81.130, P<0.05）. Conclusion：The drinking water in Dali from 2010 to 2013 is inordinately polluted by
microorganism to somewhat different extent. Though the qualified rate has an ascending trend year by year, yet it is still low. So water
quality needs to be further improved.

Profile of Occupational Exposure in an Infectious Disease Hospital

Xue Lian, Wu Guojing, Wu Kunli

2017, 2(8):  75-78. 

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Objective：To analyze the risk factors of occupational exposure among the medical staff in infectious disease hospitals.
Methods：A retrospective analysis was performed on the occupational exposure profile from January 2013 to December 2013 by using
a self-designed occupational exposure registry. Results：The incidence of hospital occupational exposure was the highest among
clinical nurses, and the highest rate was among nurses who had worked for less than a year. The incidence of needle stick injury was up
to 74.5%. The rate of contact to HIV and HBV accounted for 37.3%, and 14.5% of the occupational exposures happened for unknown
reasons. 59（53.6%） medical staff in the Infectious Disease Department experienced occupational exposure. Conclusion：It is
important to promote the concept of safe injection, strengthen management of occupational exposure to blood-borne pathogens and
explore individualized patterns of occupational protection education and training for all the staff.

Application of PDCA Cycle in Standardized Residency Training

Li Rui, Li Qiaofen, LiWei, Sun Liqiong

2017, 2(8):  79-83. 

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Objective：To study the improvements of the test scores and satisfaction as a result of the use of PDCA cycle in
Standardized Residency Training. Methods：Questionnaires were administered to 322 teachers of standardized residency training for
two times. A quality improvement team was set up, and the PDCA management method was applied to improve the scheme of the first
batch. Results：Through the statistical analysis of the data, we found that the test scores and satisfaction of the second batch was better
than the first one. Conclusion：PDCA management method is helpful to establish a scientific and effective mechanism for the training
staff, and is effective in improving the test scores and satisfaction in standardized residency training.

An Analysis of the Influential Factors of Colostomy Complications

Jin Zhuxuan, Song Zheng

2017, 2(8):  84-86. 

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Objective：To analyze the relationship between the influential factors and the occurrence of colostomy complications．
Methods：A retrospective analysis was performed of the medical records of 225 patients who had undergone colostomy in the Affiliated
Hospital of Dali University from 2013 to 2015 to examine the relationship between the incidence of colostomy complications and such
factors as age, gender, nutritional status, albumin and types of stoma. Results：Fifty- one patients of the total number had
complications with an incidence rate of 22.7%: colostomy complications correlated positively with age; gender had no significant effect
on incidence of complications. The incidence rate of complications in the patients with normal nutrition was lower than in the patients
with consumption of thin or obese; the lower albumin was, the higher the possibility of occurrence of stoma complications was; the
complication rate in loop colostomy was higher than that in end stoma. Conclusion：Colostomy complications are closely associated
with age, nutritional status, albumin and types of stoma. Good perioperative preparations need to be done based on specific individual
patient situation, which can help reduce the possibilities of stoma complication.

Observation of the Effects of Two Venipuncture Methods Used in Intravenous Infusion for
Senile Patients with Edema

Li Limei, Ma Guanglan, Zhang Guixian

2017, 2(8):  87-88. 

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Objective：To observe the effects of two kinds of vein puncture in elderly patients with pitting edema. Methods: Fifty
patients with pitting edema hospitalized in The Sixth Affiliated Hospital of Dali University from January 2014 to June 2015 were
selected and randomly divided into the control group and the observation group, each with 25 patients. The patents of the control group
were required to clench the fist of the arm to which the tourniquet was applied for venipuncture, while in the observation group patients
didn't clench the fists and tourniquets were not applied, but instead we only used 75% alcohol for external application. Then we
compared the first-stick success rate, degree of pain felt by patients and their satisfaction between the two groups. Results: The
differences between the two groups were statistically significant（P＜0.01）. The result of the observation group was better than that of
the control group. Conclusion：The method without the use of tourniquets and with no requirements of clenching the fist for
venipuncture in the senior patien

Effect of Chewing Gum on the Recovery of Bowl Function after Colorectal Surgery: A Meta-analysis

He Xiaofeng, Tang Jun

2017, 2(8):  89-93. 

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Objective：To evaluate the efficacy of chewing gum on the recovery of bowel function after colorectal surgery. Methods：
A thorough search of the data from Cochrane Library, PubMed, Embase, Medline, Web of Science, CBM, CNKI, Wanfang and Vip was
performed. Randomized controlled trials（RCT）about effect of chewing gum on bowel function after colorectal surgery were collected,
and meta-analysis was conducted with RevMan 5.3 software. Results：Fourteen RCT including 1 041 patients were enrolled in this
study. The Meta-analysis revealed that chewing gum could significantly reduce the time to the first passage of flatus［MD=-9.60 h,
95%IC（-15.22,-3.98）, P＜0.001］, the time to first defecation［MD=-17.19 h, 95%IC（-26.04, -8.34）, P＜0.001］, and postoperative
hospital stay after colorectal surgery［MD=-1.16 d, 95%IC（-2.01, -0.32）, P＜0.05］. Conclusion：Chewing gum is an effective way
to promote the recovery of bowel function after colorectal surgery.

Experience and Improvement of Surgical Practice Teaching for Foreign Students at Dali University

Yang Li, Wu Xuedong, Li Shaobo, Liu Rengui, Wang Ning, Miao Zheng

2017, 2(8):  94-97. 

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Through investigating the situation of surgical practice teaching for foreign students in Dali University, we have found
there were some problems needing to be solved, such as lax discipline, communication barriers between teachers and students, foreign
students' poor understanding of knowledge and the monotonous teaching form and method, etc. After feedback and summary, the
followings solutions have been recommended: improving the teacher's English communication skills, establishing institutional
constraints, helping students form good study habits and intensifying teaching materials, teaching form and methods. Our study proves
that the above-mentioned measures have indeed improved the quality and effect of teaching.

Construction of a Clinical Skills Training Recording-broadcasting System Based on the Campus Network

Li Wei, Zhu Li, Li Mei

2017, 2(8):  98-100. 

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The aim of this study was to construct a clinical skills training recording-broadcasting system based on the campus
network. The system can help to realize a live teaching based on the educational video application platform; it can meet the needs of the
construction of excellent courses and video open class, too. Microteaching and flipped classroom model can also be applied in clinical
skill training with the help of this recording system.