Epidemiological Investigation on Hepatitis E Virus Infection in Pigs Fed in Dali

Abstract

Abstract:

〔Abstract〕Objectives: To survey the prevalence of swine hepatitis E virus in pigs in Dali, and provide a theoretical basis for
preventing and controlling swine hepatitis e virus. Methods: Enzyme-linked immunosorbent assay（ELISA）was used to detect HEV-
IgG and HEV-IgM in 510 serum samples, the immunohistochemical staining method was used to detect HEV antigen in 152 liver
samples, and the liver samples were histopathologically sectioned and stained by hematoxylin and eosin for microscopic observations.
Results: ① The positive rates of HAV-IgG and HEV-IgM were 17.5%（89/510）and 6.3%（32/510）, respectively, none of the samples
was both HEV- IgG and HEV- IgM positive, and the positive rate of anti- HEV was 23.7%（121/510）. ② Histopathological
observations showed different degrees of pathological changes could be found in 68.4%（104/152）liver tissue samples. ③ The results of
immunohistochemical stains indicated that the HEV antigen positive rate was 63.2%. Conclusion: There is a high prevalence of HEV
in swine herds in Dali, and relevant government departments should attach importance to it and take corresponding measures.

Key words: 〔Key words〕hepatitis E virus （HEV）, swine, immunohistochemistry, histopathology, Dali

CLC Number:

Ai Zhiqiong, Li Lijuan, Li Yong, Min Mengya, Shen Yuanying（. Epidemiological Investigation on Hepatitis E Virus Infection in Pigs Fed in Dali[J]. , 2018, 3(10): 63-66.

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