Abstract:

〔Abstract〕Objective: To establish UPLC fingerprints of Periplaneta americana to assess the quality of different batchs of CⅡ-3
samples. Methods: The analysis of Periplaneta americana extract CⅡ-3 was performed in Phenomenex Gemini 5u C18 110A column
（250 mm × 4.6 mm）and the column temperature was 28 ℃. And acetonitrile-water（0.1% formic acid）was the mobile phase of
gradient elution, set at 214 nm wavelength. The quality of C Ⅱ- 3 was assessed according to the similarity evaluation and the
chemometrics method. Results: The UPLC fingerprints of 12 batches of CⅡ-3 were established. There were 21 common peaks and
fingerprints similarity of 11 batches samples was greater than 0.900. Conclusion: It is the first time that the UPLC fingerprint of CⅡ-3
has been established, which provides a scientific basis for the quality control of Periplaneta americana extract CⅡ-3.

Key words: 〔Key words〕Periplaneta americana L., CⅡ-3, fingerprints, similarity