Journal of Dali University ›› 2021, Vol. 6 ›› Issue (2): 25-28.DOI: 10. 3969 / j. issn. 2096-2266. 2021. 02. 005
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Wang Yongkuan1, Tian Xinyan2, Su Juan2, Pang Bo1, Zhou Ping1*
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Abstract: Objective: To explore the protective effects of Diaphragma juglandis Fructus extract pretreatment on rat hepatic ischemiareperfusion injury（HIRI）. Methods: 30 SD male rats were randomly divided into the sham operation group（S group）, the ischemiareperfusion group（I/R group）and the drug treatment group（DJ group）, with 10 rats in each group. The DJ group was given the administration intervention of the extracts of Diaphragma juglandis Fructus, and the S, I/R groups were given the same volume of 0.9% sodium chloride solution. After 14 days, a rat HIRI model was established, except for the S group. After 24 hours, samples were taken to detect the liver function and biochemical indexes of ischemic tissue, and the pathological changes of liver tissue were observed by hematoxylin-eosin staining. Results: Compared with S group, the activities of AST, ALT and XOD in liver tissues of I/R group increased （P < 0.01）, the contents of MDA, Hcy and NO increased（P < 0.05 or P < 0.01）, while SOD and GSH-Px levels decreased（P < 0.01）. Observed under a fluorescence microscope, the rat liver cells in the I/R group were obviously degenerated and necrotic, while the abovementioned conditions in the DJ group pretreated by the administration were improved to varying degrees. Conclusion: The pretreatment of the ethyl acetate extract of Diaphragma juglandis Fructus has a certain antioxidant effects on HIRI in rats.
Key words: hepatic ischemia-reperfusion injury, Diaphragma juglandis Fructus, oxidative stress
CLC Number:
R965.1
Wang Yongkuan, Tian Xinyan, Su Juan, Pang Bo, Zhou Ping. Preliminary Study on the Protective Effects of Diaphragma juglandis Fructus Extract on HIRI in Rats[J]. Journal of Dali University, 2021, 6(2): 25-28.
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http://journal15.magtechjournal.com/Jwk_dlxyzk/EN/Y2021/V6/I2/25
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