Abstract: Objective: To study the protective effect of Xigui polysaccharide on sleep deprivation induced injury. Methods: Mice
were randomly divided into a control group, a sleep deprivation 48 h group, a diazepam group（10 mg/kg）, and high, medium and low
dose groups of Xigui polysaccharide［400, 200, 100 mg /（kg·d）］. The diazepam group was given drugs on the last day, and the other
groups were given corresponding drugs by gavage for 7 days. After the last administration, malondialdehyde（MDA）, nitric oxide（NO）,
glutathione peroxidase（GSH-Px）activity, liver and kidney glycogen content, thymus and spleen index, and acetylcholinesterase
（AChE）activity were measured. Results: Compared with the sleep deprivation 48 h group, the content of MDA and NO, the activity of
GSH-Px were decreased, the glycogen formation ability of liver and kidney was restored, the index of thymus and spleen was increased,
and the AChE activity was decreased, the effect of high dose group was better（P＜0.01）. Conclusion: Xigui polysaccharide has
protective effects on oxidative damage, energy reserve, immunity, learning and memory function after sleep deprivation.

Key words: Xigui polysaccharide, sleep deprivation, oxidative damage