Effects of Periplaneta americana Extracts on Apoptosis of Hippocampal Neuronal Cell HT22

Abstract

Abstract: 〔Abstract〕Objective: To observe the effects of components extracted from Periplaneta americana with different solvents on the
apoptosis of mouse hippocampal neuronal cell lines HT22 cells cultured in vitro. Methods: Water extract, 40% alcohol extract, 60%
alcohol extract, 95% alcohol extract and crude extract of Periplaneta americana were prepared. And the above five components were
used to intervene in the HT22 cells cultured in vitro. The solvent control group and Periplaneta americana extract groups with final
concentrations of 60, 80, 100 and 120 μg/mL were set for each component. Flow cytometry was used to detect the effect of each
component of Periplaneta americana extract on HT22 cell apoptosis. Results: Compared with the solvent control, the crude extract and
the 95% alcohol extract of Periplaneta americana promoted the apoptosis of HT22 cells, and the differences were statistically
significant（P < 0.05）; the water extract and the 40% alcohol extract of Periplaneta americana inhibited apoptosis of HT22 cells, and
the differences were statistically significant（P < 0.05）; 60% ethanol extract of Periplaneta americana has no obvious effect on cell
apoptosis, the differences were not statistically significant（P>0.05）. Conclusion: The different components of Periplaneta americana
extract have different effects on the apoptosis process of mouse hippocampal neurons.

Key words: 〔Key words〕hippocampus, neuron, Periplaneta americana, apoptosis, aging

CLC Number:

R741.02

Yang Li, Zhu Jianhua. Effects of Periplaneta americana Extracts on Apoptosis of Hippocampal Neuronal Cell HT22[J]. Journal of Dali University, 2021, 6(4): 23-26.

References

〔1〕杨雪琪，蔡洋萍. 老龄化加速背景下我国老年人口金融服
务优化问题研究〔J〕. 中国中小企业，2019（6）：109-110.
〔2〕VANGUILDER H D，FREEMAN W M. The Hippocampal
Neuroproteome with Aging and Cognitive Decline：Past
Progress and Future Directions〔J〕. Front Aging Neurosci，
2011（3）：8.
〔3〕FIOCCO A J，YAFFE K. Defining Successful Aging：The
Importance of Including Cognitive Function Over Time〔J〕.
Arch Neurol，2010，67（7）：876-880.
〔4〕林喆，李全学，徐静，等. 美洲大蠊化学成分和生物活性
研究进展〔J〕. 中草药，2017，48（17）：3645-3649.
〔5〕LI D C，LI W，CHEN Y H，et al. Anti-Fibrotic Role and
Mechanism of Periplaneta americana Extracts in CCl 4 -in⁃
duced Hepatic Fibrosis in Rats〔J〕. Acta Biochim Biophys
Sin，2018，50（5）：491-498.
〔6〕DRISCOLL I，HOWARD S R，STONE J C，et al. The Aging
Hippocampus：A Multi-Level Analysis in the Rat 〔J〕. Neu⁃
roscience，2006，139（4）：1173-1185.
〔7〕WIMMER M E，HERNANDEZ P J，BLACKWELL J，et al.
Aging Impairs Hippocampus Dependent Long Term Memo⁃
ry for Object Location in Mice〔J〕. Neurobiol Aging，2012，
33（9）：2220-2224.
〔8〕SIMIĆ G，KOSTOVIĆ I，WINBLAD B，et al. Volume and
Number of Neurons of the Human Hippocampal Formation
in Normal Aging and Alzheimer′s Disease〔J〕. J Comp Neu⁃
rol，1997，379（4）：482-494.
〔9〕KRIL J J，HODGES J，HALLIDAY G. Relationship between
Hippocampal Volume and CA1 Neuron Loss in Brains of
Humans with and without Alzheimer′s Disease〔J〕. Neurosci
Lett，2004，361（1）：9-12.
〔10〕KIRAY M，BAGRIYANIK H A，PEKCETIN C，et al. De⁃
prenyl and the Relationship between Its Effects on Spatial
Memory，Oxidant Stress and Hippocampal Neurons in
Aged Male Rats 〔J〕. Physiol Res，2006，55（2）：205-212.
〔11〕刘青，李永，盛世美，等. 枸杞多糖改善衰老小鼠学习记
忆的作用〔J〕. 兰州大学学报（医学版），2020，46（3）：
38-42.
〔12〕吴建新，钮荣祥，黄秀群，等. 心脉龙注射液抗衰老作用
的实验研究〔J〕. 贵阳医学院学报，2002，27（2）：125-
127.
〔13〕CHEN Z J，HU Y C，LI J，et al. A Feasible Biocompatible
Hydrogel Film Embedding Periplaneta americana Extract
for a Cute Wound Healing〔J〕. Int J Pharm，2019，571
（11）：118707.
〔14〕焦春香，张成桂，刘光明，等. 美洲大蠊提取物中抗衰老
活性部位抗氧化活性的初步分析〔J〕. 时珍国医国药，
2011，22（6）：1389-1391.
〔15〕LINSS，LIUCX，WANGXL，etal.InterventionMechanisms
of Xinmailong Injection，A Periplaneta americana Extract，
on Cardiovascular Disease：A Systematic Review of Basic
Researches〔J〕. Evid Based Complement Alternat Med，
2019，2019：8512405.

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