Journal of Dali University ›› 2021, Vol. 6 ›› Issue (8): 1-6.DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 001

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Study on Reversal of Multidrug Resistance of Drug-Resistant Cell Lines by Polypeptide PAE2 from Periplaneta americana

Wang Yanquan1, Zhang Honghan1, Lü Hong1, Li Cailin1, Najib Mohammerd1, Peng Fang1, Wu Dingyu2*   

  1. (1. College of Pharmacy, Dali University, Dali, Yunnan 671000, China; 2. The Asset and Laboratory Management Division,Dali University, Dali, Yunnan 671000, China)
  • Received:2021-02-24 Revised:2021-03-31 Online:2021-08-15 Published:2021-09-28

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Abstract: Objective: To investigate the effect and mechanism of Periplaneta americana polypeptide PAE2 on reversing multi-drug
resistance of human hepatocellular carcinoma drug-resistant cell line BEL-7402/5-FU. Methods: Human hepatocellular carcinoma
sensitive cell line BEL-7402 and human hepatocellular carcinoma drug-resistant cell line BEL-7402/5-FU were used as the research
objects. The cell viability was detected by methyl thiazolyl tetrazolium (MTT) assay. The mRNA and protein expression levels of
glutathione S-transferase- π (GST- π ), topo-isomerase type (Topo ) and protein kinase C (PKC) in cells were detected by real
time- polymerase chain reaction(RT-PCR) and immunocytochemical staining. Results: The Periplaneta americana polypeptide
PAE2 can effectively reverse the multi-drug resistance of human hepatocellular carcinoma drug-resistant cells BEL-7402/5-Fu, and
can down-regulate the mRNA and protein expression levels of multi-drug resistance related enzymes like PKC, GST- π and Topo .
Conclusion: The mechanism of PAE2 on reversing multidrug resistance in vitro may be related to the down-regulation of multidrug
reactance-related enzymes.

Key words: hepatocellular carcinoma, multidrug resistance, cross-resistance, polypeptide PAE2 from Periplaneta americana

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