Journal of Dali University

Study on Reversal of Multidrug Resistance of Drug-Resistant Cell Lines by Polypeptide PAE2 from Periplaneta americana

Wang Yanquan, Zhang Honghan, Lü Hong, Li Cailin, Najib Mohammerd, Peng Fang, Wu Dingyu

2021, 6(8): 1-6. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 001

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Objective: To investigate the effect and mechanism of Periplaneta americana polypeptide PAE2 on reversing multi-drug
resistance of human hepatocellular carcinoma drug-resistant cell line BEL-7402/5-FU. Methods: Human hepatocellular carcinoma
sensitive cell line BEL-7402 and human hepatocellular carcinoma drug-resistant cell line BEL-7402/5-FU were used as the research
objects. The cell viability was detected by methyl thiazolyl tetrazolium (MTT) assay. The mRNA and protein expression levels of
glutathione S-transferase- π (GST- π ), topo-isomerase type (Topo ) and protein kinase C (PKC) in cells were detected by real
time- polymerase chain reaction(RT-PCR) and immunocytochemical staining. Results: The Periplaneta americana polypeptide
PAE2 can effectively reverse the multi-drug resistance of human hepatocellular carcinoma drug-resistant cells BEL-7402/5-Fu, and
can down-regulate the mRNA and protein expression levels of multi-drug resistance related enzymes like PKC, GST- π and Topo .
Conclusion: The mechanism of PAE2 on reversing multidrug resistance in vitro may be related to the down-regulation of multidrug
reactance-related enzymes.

Preliminary Study on Drug Release through BCP Artificial Bones Loaded with#br# Rifampicin and Isoniazid in Rabbits

Li Zhongjie, Liu Feifei, Ruan Yushan, Ding Kai, Lei Yanfa, He Ying, Li Shaobo

2021, 6(8): 7-10. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 002

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Objective: To observe the features of drug release through bidirectional calcium phosphate (BCP) artificial bones loaded
with rifampicin (RFP) and isoniazid (INH) in rabbits. Methods: BCP artificial bones were immersed in rifampicin and isoniazid
solutions under dark conditions, and then dried to obtain drug-loaded artificial bones. Direct ultraviolet-visible spectrophotometry was
used to determine the loading rate of rifampicin and isoniazid respectively. A rabbit iliac bone defect model was established, with 6 in
the blank group (BCP artificial bone group) and 18 in the experimental group (loaded with rifampicin and isoniazid BCP artificial bone
group). Samples were taken on the 1st, 3rd, 7th, 14th, 28th and 56th days after the surgery, and the plasma and local tissue drug concentrations
were determined. Results: The drug loading rate of rifampicin by BCP artificial bone was (5.98±0.80) %, and the drug loading rate of
isoniazid was (15.08±2.22) %. There was a statistical difference in drug concentrations between local tissue and plasma samples at the
same time point, with the drug concentration in local tissue significantly higher than that in plasma (P<0.05). Conclusion: Drug-loaded
BCP artificial bones have a slow-release effect of anti-tuberculosis drugs and can be used as a carrier for subsequent studies on the
slow-release of anti-tuberculosis drugs.

Application of Network Pharmacology to Predict the Mechanism of Semen Cassiae#br# in the Treatment of Hypertension

Gao Xiaomin, Zhao Qin

2021, 6(8): 11-15. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 003

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Objective: To apply network pharmacology to predict the chemical components and mechanism of Semen Cassiae in the
treatment of hypertension. Methods: To search for the chemical constituents and corresponding targets of Semen Cassia through
the TCMSP database, to search for disease-related targets in the GeneCards database, to use Cytoscape 3.6.1 software to create a
drug-component-disease target regulatory network, to use the STRING database to construct protein-protein interaction network , and
to do GO function and KEGG pathway enrichment analyses of Chinese medicine-disease targets through DAVID database. Results:
11 chemical ingredients of Semen Cassia for the treatment of hypertension and 46 effective targets were screened out. The signal
pathways mainly include calcium signal pathway, thyroid hormone signal pathway, and so on. Conclusion: The chemical ingredients in
Semen Cassia may act on ESR1, JUN, MAPK14 and other targets through calcium signaling pathway, and thyroid hormone signaling
pathway to play a hypotensive effect.

Study on the Mechanism of Ophiocordyceps sinensis in Treating Liver Cirrhosis Based on Network Pharmacology

Li Jie, Chen Yichun, Li Jufa, Chen Lintao, Wang Jiazhe, Jiang Yina, Zhang Bintao

2021, 6(8): 16-21. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 004

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Objective: To analyze and explore the main active components, main targets, key pathways and mechanisms of
Ophiocordyceps sinensis in the treatment of liver cirrhosis by using network pharmacology methods. Methods: The potential targets
and main active components of Ophiocordyceps sinensis were captured from the TCMSP database; the main targets of liver cirrhosis were
obtained from the GeneCards database; a component-target network was constructed by Cytoscape 3.2.1 software; a protein-protein
interaction network(PPI) was constructed by STRING database; DAVID database was used for GO function enrichment and KEGG
pathway enrichment analyses. Results: 87 potential targets, 6 main active ingredients and 56 liver cirrhosis targets were obtained. The
PPI network contained 45 proteins, involving MAPK1, CASP8, etc. KEGG pathway enrichment analysis yielded 44 signal pathways
(FDR<0.01), involving pathways in cancer, Hepatitis B, etc, and GO functional enrichment analysis yielded 23 entries (P<0.01).
Conclusion: This study uses network pharmacology to preliminarily predict the mechanism of Ophiocordyceps sinensis in the
treatment of liver cirrhosis, which provides new ideas for subsequent in-depth research.

A Meta-Analysis of the Efficacy and Safety of Xinmailong Injection in the Treatment of Chronic Heart Failure

2021, 6(8): 22-30. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 005

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Objective: To evaluate the efficacy and safety of Xinmailong injection in the treatment of chronic heart failure(CHF).
Methods: The randomized controlled trial of Xinmailong injection in the treatment of CHF from the establishment of the database to
January 20, 2020 was searched in CNKI, VIP Chinese database, Wanfang database, PubMed, Cochrane Library and other databases.
Then, the appropriate literatures were selected, and the total clinical effective rate, left ventricular ejection fraction(LVEF), left ventricular
end diastolic diameter(LVEDD), 6 minutes walking distance(6MWD), N-terminal B-type brain natriuretic peptide(NT-proBNP), brain
natriuretic peptide(BNP) and the incidence of adverse reactions were Meta analyzed by Revman 5.3 software. Results: A total of 433
articles were retrieved, and finally fifteen RCTs with 1 996 CHF patients met the inclusion criteria and were included in the present
Meta-analysis. The results showed that the level of BNP，LVEDD, and NT-proBNP were significantly reduced and the LVEF, 6-MWD,
total clinical effective rate of CHF patients were significantly increased without causing obvious adverse reactions. Conclusion: In the
existing literature, Xinmailong injection can significantly improve the clinical indicators of CHF patients, improve the clinical efficacy,
and the incidence of adverse reactions is mild. However, the quality of literature is generally low, the information provided is insufficient,
and the source of literature bias is difficult to determine. Therefore, a large number of accurate and objective research is needed.

Evaluation of Rational Clinical Application of Ceftazidime for Injection in 252 Inpatients

Bu Wei, Wang Yuhe, Gong Lei

2021, 6(8): 31-34. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 006

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Objective: To evaluate the rationality of clinical use of ceftazidime and to provide reference for clinical standardization
and rational use of ceftazidime. Methods: A total of 252 hospitalized patients who received ceftazidime injection were selected from
July 2019 to September 2019 in the Affiliated Hospital of Zunyi Medical University (hereinafter referred to as “our hospital”). The
rationality of medication was evaluated in terms of indications, dose regimen, microbiological examination, and combination of drugs.
Results: The drugs of utilization index (DUI) of ceftazidime for injection in our hospital was ＜ 1. Among the drug use of 252 cases,
214 cases (84.92%) were rational and 38 cases (15.08%) were irrational; 146 cases (57.94%) received routine etiological examinations,
including 84 blood samples (57.53%) and 42 sputum samples (28.77%); 67 cases (26.59%) received combined use of the two drugs,
and 1 case (0.40%) was incompatible. The patients used ceftazidime for injection were mainly hospitalized in the respiratory and critical
medical area, involving respiratory diseases such as pneumonia and asthma, with a total number of 71 cases (28.18%). Conclusion: The
clinical use of ceftazidime for injection in our hospital is basically rational, but there were still a few cases with inappropriate drug use.

Special Comments on Clinical Rational Use of Methylprednisolone in Our Hospital

2021, 6(8): 35-38. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 007

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Objective: To evaluate the clinical application of methylprednisolone in Lincang People s Hospital (hereinafter referred
to as “our hospital”)， in order to improve the rationality of clinical application of glucocorticoid. Methods: A total of 240 medical
records of discharged patients who used methylprednisolone between 2018 and 2019 in our hospital were selected, and the rationality
of their medication was analyzed. Results: Of the 240 medical records, 21 were with irrational use of methylprednisolone (8.75%); the
drug was most frequently used in pediatrics (17.92%); the highest proportion of drug use was for chemotherapy for malignant tumors
(22.50%); methylprednisolone sodium succinate 40 mg was most frequently used (90.42%). Conclusion: Clinical pharmacists should
strengthen the special comments on glucocorticoid drugs, so as to improve the rationality of clinical application of such drugs and ensure
the safety of patients.

Clinical Application Analysis of Setrons in Our Hospital from 2017 to 2019

Liu Zuxiang, Tu Wenlian, Cao Wei

2021, 6(8): 39-43. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 008

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Objective: To understand the use and trend of setron drugs in the First People s Hospital of Yunnan Province (hereinafter
referred to as “our hospital”）, and provide references for the rational use of such drugs in clinical practice. Methods: The electronic
medical records of 300 cases of setrons used in our hospital information management system (HIS) from 2017 to 2019 were selected
and analyzed from the aspects of variety, sales amount, frequency of drug use (DDDs), daily average cost and unreasonable use of
drugs, etc. Results: Tropisetron injection ranked first in sales sum and DDDs. There were 49 cases of irrational use of the drug,
accounting for 16.33% of the total cases. Conclusion: The use of setron drugs in our hospital is increasing year by year, and the trend
of drug use basically meets the needs of treatment, but the problem of incomplete dosage forms and varieties needs to be improved.

Analysis of Adverse Reactions and Influencing Factors of Tigecycline in the Treatment of Severe Pneumonia

Tu Xiongwen

2021, 6(8): 44-46. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 009

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Objective: To analyze the adverse reactions and influencing factors of tigecycline in the treatment of severe pneumonia.
Methods: 124 patients with severe pneumonia who were treated with tigecycline in Yijishan Hospital,Wannan Medical College from
July 2019 to June 2020 were selected, and the relevant clinical data were counted. Through the analysis of different indexes of patients
with and without adverse reations, the influencing factors of adverse reactions of tigecycline were understood. Results: Among the 124
patients, 35 cases had adverse reactions, the incidence rate was 28.23%, including 12 cases of liver function injury, 2 cases of rash, 1
cases of edema, 10 cases of drug-induced thrombocytopenia, 9 cases of digestive system problems, 1 cases of thrombocytopenia and
liver function injury. There were significant differences in age, alanine aminotransferase (ALT), high blood pressure, smoking
and exercise between patients with and without adverse reactions (P< 0.05), but there were no significant differences in gender, body
mass index (BMI), drinking and aspartate aminotransferase (AST) (P> 0.05). Conclusion: The main adverse reactions of tigecycline
in the treatment of severe pneumonia are edema, liver function damage, rash, thrombocytopenia, thrombocytopenia and liver function
damage. The occurrence of adverse reactions is related to the patient s age, ALT, high blood pressure, smoking and exercise.

Analysis of the Pulmonary Infection of Multidrug-Resistant Organism in Our Hospital s#br# Comprehensive Internal Medicine Department in Recent Years

Duan Liping

2021, 6(8): 47-49. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 010

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Objective: To analyze the pathogens of multidrug-resistant organism (MDRO) in the comprehensive internal medicine
department of Dali Bai Autonomous Prefecture People s Hospital ( hereinafter referred to as “our hospital” ), and to explore the risk
factors and propose effective nursing strategies. Methods: From January 2010 to June 2017, 82 patients with MDRO lung infection were
treated in our hospitals comprehensive internal medicine department. The types of pathogens and their therapeutic effects were analyzed.
Meanwhile, effective nursing strategies were put forward. Results: In our hospital, the majority of MDRO were Pseudomonas
aeruginosa (37.80%), Klebsiella pneumoniae (26.83%), Acinetobacter baumannii (20.73%), and Staphylococcus aureus (10.98%).
Major risk factors of MDRO lung infection were irrational use of antibiotics, indwelling gastric tube, mechanical ventilation, long
hospital stay, smoking history and other underlying diseases. After targeted care, more than 79.27% of the patients with MDRO lung
infection were improved and discharged. Conclusion: Finding out risk factors, identifying pathogens, and giving effective nursing
intervention are the keys to reducing the occurrence of MDRO lung infection.

Effect of Periplaneta americana Extract on Intestinal Flora in Patients with Mental Illness

Yin Chunlei, Wang Nan, Ma Yingying, Jia Chengyi, Zhang Qian, Li Lijuan

2021, 6(8): 50-53. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 011

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Objective: To investigate whether the extract of Periplaneta americana has an effect on the intestinal flora of patients
with mental illness. Methods: Fecal samples collected from 19 patients with mental illness during the onset and control periods were
co-cultured with microorganisms, and the number of colonies was analyzed statistically. Results: The number of colonies in each of
the three samples (blank group, experimental group, and positive group) of the three types of microorganisms (aerobic bacteria,
anaerobic bacteria, fungi) was compared in pairs. The differences in the number of colonies between the blank group and the
experimental group, the blank group and the positive group ( aerobic bacteria group except female and schizophrenic characteristics )
were statistically significant (P<0.05). Conclusion: The extract of Periplaneta americana can regulate the intestinal flora of patients
with mental illness.

Study on the Correlation between Red Blood Cell Count and Hypertension at High Altitude

Hu Naiqing, Lü Jinlin, Yin Xueyan, Li Lihua

2021, 6(8): 54-57. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 012

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Objective: To study the correlation between red blood cell count and blood pressure and hypertension at high altitude.
Methods: All employees and retired workers in a factory in Dali were invited to participate in this study, and a standardized
questionnaire was used to collect the participants’ demographic information, disease history, smoking and drinking habits, family
history of hypertension and so on. SPSS 19.0 was used for multiple linear regression analysis and Logistic regression analysis.
Results: The 1 395 participants included 546 women (39.1%) and 460 hypertensive patients (33.0%). Multiple linear regression
analysis showed that after adjusting for covariates, red blood cell count was positively correlated with systolic and diastolic blood
pressure, and hemoglobin level was positively correlated with diastolic blood pressure. Logistic regression analysis found that after
adjusting for covariates, red blood cell count was independently associated with the increased risk of hypertension. For each 1×1012/L
increase in red blood cell count, the risk of hypertension increased by 92% (OR=1.920, 95%CI 1.161-3.175, P=0.011), while
hemoglobin was not associated with the risk of hypertension (P= 0.361). Conclusion: Red blood cell count was positively correlated
with blood pressure and risk of hypertension at high altitude. Long-term chronic hypoxia at high altitude may be involved in the
regulation of blood pressure and the pathogenesis of hypertension by stimulating erythrocytosis.

Feasibility Analysis of Seminal Vesicle Technique in the Treatment Refractory Prostatitis

Li Jiepeng, Li Jianjun, Luo Yong, Zhou Yukai, Suo Maohua, Xiao Yukun

2021, 6(8): 58-61. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 013

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Objective: To investigate the clinical efficacy of transurethral seminal vesicle in the treatment of refractory prostatitis.
Methods: From March 2016 to August 2019, 58 patients with refractory prostatitis who were treated in the department of urology
in the First Affiliated Hospital of Dali University were selected as the research subjects. They were randomly divided into two
groups, i.e., the treatment group and the control group. All the 28 patients in the treatment group were treated by seminal vesicle.
The 30 patients in the control group were treated with comprehensive conservative methods. Before and in the 6th months after
treatments, according to the National Institutes of Health Prostatitis Scale, International Index of Erectile Function,Self-rating
Depression Scale Score, and small patticle of lecithin and interleukin-10 levels, patients in the two groups were compared to judge the
clinical efficacy. Results: After 6 months of treatment, the improvement degree of each index in the treatment group was better than
that in the control group. Conclusion: Transurethral seminal vesiculoscopy in the treatment of refractory prostatitis is significantly better than
comprehensive conservative treatment, and can effectively improve the clinical symptoms and life quality of patients.

Case Analysis of Different Epididymal Anastomoses in the Treatment of#br# Epididymal Obstructive Azoospermia

Li Jianjun, Luo Yong, Zhang Jiule, Zhou Yukai, Li Jiepeng, Suo Maohua, XiaoYukun

2021, 6(8): 62-66. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 014

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Objective: To explore the effects of different epididymal anastomosis sites of epididymisvasovasostomy (EV) on recanalization
rate and natural pregnancy rate. Methods: From January 2015 to June 2018, patients diagnosed with concurrent EV in the department
of urology in the First Affiliated Hospital of Dali University were analyzed retrospectively according to the patients intraoperative
conditions. According to the epididymal head, body, and tail of the anastomosis site, they are divided into three groups: A, B, and C.
During the follow-up, a routine semen examination was performed to clarify the reopening situation. Their spouses pregnancy statuses
were followed up through telephone interviews. Results: 33 cases of postoperative recanalization were successful, and the postoperative
recanalization rate was 68.75%, among which the recanalization rate of the three groups A, B, and C was 33.33% (3/9), 66.67% (12/18),
and 85.71% (18/21), respectively. The difference among the groups was significant ( χ 2=7.647, P=0.017<0.05). The spouses of
20 patients were naturally pregnant, and the natural pregnancy rate was 41.67% (20/48). The pregnancy rates of spouses in groups A, B,
and C were 11.11% (1/9), 33.33% (6/18), and 61.90% (13/21) respectively. The difference among the three groups was significant
( χ 2=7.510, P=0.023<0.05). Conclusion: Under the same anastomosis conditions, the anastomosis of the body and tail can significantly
increase the recanalization rate and natural pregnancy rate compared with the head anastomosis.

Effect of Dexmedetomindine in Early Treatment of Severe Craniocerebral Injuries

Zhou Shubo, Su Qiucai

2021, 6(8): 67-69. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 015

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Objective: To discuss the safety and neuroprotecive effect of early use of dexmedetomidine on patients with severe
craniocerebral injuries. Methods: 62 cases of severe craniocerebral injuries from January 2016 to August 2018 in the department of
neurosurgery intensive care unit in Central Hospital of Yiyang City were studied. They all showed various degrees of restlessness, and
were sedated with intravenous use of dexmedetomidine after admission. Heart rate (HR), respiratory rate (R), blood oxygen saturation
(SaO2) and arterial partial pressure of oxygen (PaO2) before and 30 min, 1, 6, 12, 24 h after medication were recorded, and changes in
interleukins-6 (IL-6) and C-reactive protein (CRP) were analyzed. The drug dosage that sedated the patient to a Ramsay score of 2 to 4
was the maintenance dose. Results: All patients received satisfactory sedation after continuous pumping of dexmedetomidine, and there
was no excessive sedation. SaO2 and PaO2 were significantly increased at 6,12 and 24 hours after the treatment, and the difference was
statistically significant compared with that before treatment (P< 0.05), the R of patients at 6,12 and 24 h after the treatment were
significantly lower than those before the treatment, the difference was statistically significant (P<0.05). Conclusion: Dexmedetomidine
has a satisfactory sedative effect on patients with severe craniocerebral injury, with no respiratory depression, high safety, and could
make the patients be awaked easily when necessary, so it is suitable for short-term sedation on patients with severe craniocerebral
injury and restlessness.

Surgical Management Strategies for Asymptomatic Meckel Diverticulum in Children

He Jie, Chen Su, Wang Ning, Liu Qingling, Zhou Zhou, Wu Xuedong

2021, 6(8): 70-73. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 016

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Objective: To explore the surgical management strategy of asymptomatic Meckel diverticulum (MD) in children. Methods: The
databases of MD in children were collected from PubMed, Cochrane, CNKI and wanfang prior to March 1, 2020. The data of the
number of surgical cases, postoperative complications and the presence of gastric mucosal heterotopia were extracted through reading
and selecting articles and compared between asymptomatic and symptomatic groups with statistics. Results: There were 1 585
childhood MD cases in 24 articles included in this study collected from total 14 398 literatures in Chinese and English, among which
350 children were with asymptomatic MD. The proportion of MD resection, postoperative complications and gastric mucosal ectopia
in children were significantly lower in asymptomatic MD group than that in symptomatic MD group (P< 0.05). Conclusion: The data
suggested that it is unnecessary to resect asymptomatic MD in children preventively, but whether to resect it or not should be decided
according to the situation when MD is found in children during other abdominal operations.

Clinical Effects of Free Anterolateral Thigh Flap in Repairing Soft Tissue Defects of the Forefoot

Yang Duming

2021, 6(8): 74-76. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 017

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Objective: To explore the clinical effects of free anterolateral thigh flap in repairing soft tissue defects of the forefoot.
Methods: Free anterolateral thigh flap was used to repair 20 cases of soft tissue defects in the forefoot. The minimum flap removal
area was 4.0 cm × 3.0 cm, and the maximum was 18.0 cm × 8.0 cm. The flap donor area is sutured directly. Results: All 16 flaps survived
completely, 1 flap was completely necrotic, and 3 flaps were partially necrotic. Eighteen patients were followed up for 3-12 months.
Most of the flaps were good in appearance, and the function of the forefoot receiving area was satisfactory. Conclusion: Free anterolateral
thigh flap is effective in repairing soft tissue defects of the forefoot.

Clinical Evaluation of Laparoscopic Radical Surgery for Early Stage Cervical Cancer

Wang Bin, Yang Sili, Luo Wanxun

2021, 6(8): 77-80. DOI: ］10. 3969 / j. issn. 2096-2266. 2021. 08. 018

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Objective: To evaluate the clinical effects of laparoscopic radical surgery for cervical cancer. Methods: From October
2016 to October 2019, 25 patients with stage Ⅰ A1 to stage Ⅰ B1 LRH cervical cancer in the First People s Hospital of Dali City were
selected as the observation group and 25 patients with ARH as the control group.The surgical effects of the two groups were evaluated.
Results: There was no significant difference in the operative time and the number of pelvic lymph nodes cleared between the two
groups (P>0.05). Some indicators in the observation groups, such as the operation bleeding, exhaust time, and the hospitalization time,
were significantly lower than those of the control group. These differences were statistically significant (P< 0.05). The results showed
that the incisional margin of vagina was negative and the operation was complete. The patients followed up for 12 months were
without recurrence. Conclusion: On the basis of full notification and careful evaluation, laparoscopic radical surgery for cervical
cancer has the advantages of being minimally invasive, avoiding long abdominal incision, less bleeding, quick recovery, short hospital
stay, less postoperative pain, less trauma and so on. It has obvious advantages and important clinical application value.

Initial Treatment of Pseudomonas aeruginosa Keratitis with Topical Chloramphenicol and Effects#br# on Prognosis

Mo Ni, Zheng Zhimei, Li Tingna, Li Guixiang, Yu Deshun

2021, 6(8): 81-84. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 019

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Objective: To determine whether the initial use of chloramphenicol for empirical treatment of Pseudomonas aeruginosa
keratitis has a positive effect on its prognosis. Methods: 77 patients (77 eyes) from January 2014 to December 2019, bacterial cultured
and identified as bacterial infection by Pseudomonas aeruginosa in the ophthalmology department of Dali Bai Autonomous Prefecture
People s Hospital were retrospectively analyzed. According to the use of chloramphenicol eye drops before levofloxacin eye drops,
they were divided into chloramphenicol group and non-chloramphenicol group. The ulcer area, the initial best corrected visual acuity, the
final best corrected visual acuity, the course of the disease and the complications when starting to use levofloxacin eye drops were
recorded in details. Results: 36.36% of patients used chloramphenicol eye drops before levofloxacin drops. The ulcer area in the
chloramphenicol group was larger (P<0.05), and the initial best corrected visual acuity and final best corrected visual acuity of the two
groups had no statistically significant difference. The course of the disease was longer in the chloramphenicol group (P<0.05). The
rates of corneal pannus and corneal perforation in the chloramphenicol group and non-chloramphenicol group were 3.57% and 2.04%,
respectively (P>0.05). There was no correlation between the area of the ulcer and the initial best corrected visual acuity (r <0.2). The
length of the disease has a certain correlation with the area of the ulcer, the initial best corrected visual acuity and the final best
corrected visual acuity (0.2 <r <0.4). The final best corrected visual acuity has a strong correlation with the initial best corrected visual
acuity (r> 0.6). Conclusion: More than 1/3 Pseudomonas aeruginosa keratitis patients used chloramphenicol eye drops before using
levofloxacin eye drops, but using chloramphenicol eye drops did not improve the patient s ultimate corrective vision, shorten course, or
reduce the incidence of complications.

Effects of Dexmedetomidine on Hemodynamics and Stress Response in Patients with Lung Lavage

Fu Lijuan, Li Juanjuan, Guo Zhenhua, He Wenwei, Yang Yi

2021, 6(8): 85-87. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 020

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Objective: To observe the effects of dexmedetomidine on hemodynamics and stress response in patients with lung lavage.
Methods: Sixty patients who underwent lung lavage in the First Affiliated Hospital of Dali University were randomly divided into two
groups, i.e., the control group (group C) and the dexmedetomidine group (group D). Group D was intravenously infused with
dexmedetomidine before the induction of anesthesia; group C was given an equal volume of 0.9% sodium chloride solution. Before
infusion of dexmedetomidine or 0.9% sodium chloride solution (T0), immediately after tracheal intubation (T1), at the beginning of
left lung lavage (T2), at the beginning of right lung lavage (T3), and at extubation (T4), and 5 minutes after extubation (T5), the mean
arterial pressure (MAP) and heart rate (HR) of the two groups of patients were recorded, and plasma epinephrine and norepinephrine
levels were measured at each time point. Results: Compared with T0, MAP and HR were increased at T1 to T5 in group C. In group D,
MAP and HR increased significantly only at T4. Compared with group C, MAP and HR in group D were significantly lower at T1, T2,
T3 and T4 , the differences were statistically significant (P<0.05). Plasma epinephrine and norepinephrine in the two groups increased
at T1, T2, T3 and T4. However, compared with group C, the increase in the group D was significantly reduced in these time points,
the differences were statistically significant (P<0.01). Conclusion: Dexmedetomidine can inhibit hemodynamic fluctuations and stress
response in patients with lung lavage.

Clinical Application Value of NLR in the Etiology Diagnosis of Community-Acquired Pneumonia

Li Fuxing, He Yingchun, Yan Ping, Wang Li, Fan Yujuan, Zhao Weidong

2021, 6(8): 88-92. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 021

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Objective: To explore the clinical application value of neutrophil-lymphocyte ratio (NLR) in the etiological diagnosis
of community-acquired pneumonia. Methods: The routine blood indices, CRP and the concentration of PCT were retrospectively
analyzed in 47 patients with streptococcus pneumonia (SP), 50 patients with mycoplasma pneumonia (MP) and 64 patients with viral
pneumonia (VP) within 48 hours after admission to hospital. NLR <5 was selected as cut-off value, and the results were analyzed by
statistical methods. Results: Compared with those in MP group and VP group, the patients in SP group had higher levers of white
blood cell counts, NLR, NLR<5, and PCT, the differences were statistically significant (P<0.05). NLR<5 had the best diagnostic
efficacy in distinguishing between SP and MP (AUC=0.92), VP (AUC=0.74), and non-SP (AUC=0.84), respectively. Conclusion:
NLR can distinguish bacterial infection from non-bacterial infection in the early stages. At the level of NLR<5, non-bacterial infection
such as mycoplasma or virus should be considered, which can better guide clinical medication.

Clinical and Laboratory Diagnosis Analysis of 73 cases of Tsutsugamushi Disease in Anning Area

Xu Wenyan, Liu Qiuhong, Ma Yunlin

2021, 6(8): 93-96. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 022

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Objective: To explore the clinical characteristics and laboratory characteristics of 73 cases of tsutsugamushi disease in
Anning area, as well as the risk factors of severe tsutsugamushi disease. Methods: The clinical characteristics, laboratory characteristics,
and treatment of 73 cases of tsutsugamushi disease hospitalized in Yunnan Kungang Hospital from January 2015 to December 2018
were retrospectively analyzed. According to the criteria of severe diagnosis, the patients were divided into 18 cases in the severe
group and 55 cases in the non-severe group. The clinical characteristics and laboratory results of the two groups were compared to
explore the risk factors associated with the occurrence of severe tsutsugamushi disease. Results: The incidence of 73 cases of tsutsugamushi
disease was mainly in summer and autumn, and the onset time was concentrated in July to October every year. Clinical features: all
patients had fever, and 87.7% of patients has eschar. Other atypical clinical manifestations: sick, abdominal pain, and diarrhea. Positive
laboratory indicators associated with the incidence of tsutsugamushi disease have elevated ALT, AST, LDH, PCT and CRP, decreased
eosinophils, and abnormal lymphocytes. PLT reduction, CRP, PCT, AST, and LDH elevation were statistically significant between the
severe tsutsugamushi disease group and the non-severe tsutsugamushi disease group (P<0.01). Conclusion: In addition to high fever,
tsutsugamushi disease has a variety of clinical manifestations, and there are a variety of laboratory abnormalities. Clinicians need to
carefully ask about the medical history, do comprehensive analyses of various laboratory data during the high seasons of tsutsugamushi
disease and find the eschar of the hidden parts of the body to avoid missed diagnosis and misdiagnosis of tsutsugamushi disease; patients
with severe tsutsugamushi disease are prone to PLT reduction and increased CRP, PCT, AST and LDH. Active early treatment should
be carried out to reduce the mortality of severe tsutsugamushi disease.

Cause Analysis and Preventive Measures for the Fallings of 41 Inpatients in Cardiovascular Department

Gao Lizhen, Yang Zhengyi, Zhang Mingxiong

2021, 6(8): 97-100. DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 023

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Objective: To analyze the specific situation and related risk factors for the falling of cardiovascular patients, so as to
provide reference for the formulation of effective preventive measures. Methods: Through retrospective analysis, 41 cases who fell in
the department of cardiovascular in the First People s Hospital of Yunnan Province from January 2015 to April 2020 were analyzed.
Results: The scores of self-care ability and Morse Fall Scale (MFS) were (43.75±8.43) and (57.43±9.47), respectively. Medication use,
accompanying or not, scores of MFS, and the types of diseases were the risk factors for the falling (P<0.05). Conclusion: To prevent
falling of hospitalized cardiology patients, targeted and individualized preventive measures should be taken for high-risk patients in
terms of age, falling time, and falling location.

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