Journal of Dali University ›› 2021, Vol. 6 ›› Issue (8): 7-10.DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 002

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Preliminary Study on Drug Release through BCP Artificial Bones Loaded with#br# Rifampicin and Isoniazid in Rabbits

Li Zhongjie1, Liu Feifei1, Ruan Yushan1, Ding Kai2, Lei Yanfa2, He Ying3, Li Shaobo2*   

  1. (1. Department of Spinal Surgery, The First Affiliated Hospital of Dali University, Dali, Yunnan 671000, China; 2. Clinical College,
    Dali University, Dali, Yunnan 671000, China; 3. College of Pharmacy, Dali University, Dali, Yunnan 671000, China)
  • Received:2020-05-06 Revised:2020-12-10 Online:2021-08-15 Published:2021-09-28

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Abstract: Objective: To observe the features of drug release through bidirectional calcium phosphate (BCP) artificial bones loaded
with rifampicin (RFP) and isoniazid (INH) in rabbits. Methods: BCP artificial bones were immersed in rifampicin and isoniazid
solutions under dark conditions, and then dried to obtain drug-loaded artificial bones. Direct ultraviolet-visible spectrophotometry was
used to determine the loading rate of rifampicin and isoniazid respectively. A rabbit iliac bone defect model was established, with 6 in
the blank group (BCP artificial bone group) and 18 in the experimental group (loaded with rifampicin and isoniazid BCP artificial bone
group). Samples were taken on the 1st, 3rd, 7th, 14th, 28th and 56th days after the surgery, and the plasma and local tissue drug concentrations
were determined. Results: The drug loading rate of rifampicin by BCP artificial bone was (5.98±0.80) %, and the drug loading rate of
isoniazid was (15.08±2.22) %. There was a statistical difference in drug concentrations between local tissue and plasma samples at the
same time point, with the drug concentration in local tissue significantly higher than that in plasma (P<0.05). Conclusion: Drug-loaded
BCP artificial bones have a slow-release effect of anti-tuberculosis drugs and can be used as a carrier for subsequent studies on the
slow-release of anti-tuberculosis drugs.

Key words: rifampicin, isoniazid, drug-loaded, BCP artificial bone

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