Expression and Significance of THBS2 Gene mRNA in Human Malignant Pleural Mesothelioma Based on Tumor Databases

Abstract

Abstract:

〔Abstract〕 Objective：To analyze the expression level and clinical significance of thrombospondin-2 （THBS2） gene mRNA in patients with malignant pleural mesothelioma （MPM） based on tumor databases. Methods： The difference of THBS2 mRNA expression between MPM and normal tissues was analyzed by Oncomine database； and the correlation between THBS2 mRNA expression and clinicopathological parameters of MPM patients was analyzed by TCGA database and UALCAN database. Kaplan- Meier survival model was constructed to analyze the prognostic effect of THBS2 gene expression on MPM patients； COX regression model was constructed to explore the independent risk factors affecting the prognosis of MPM patients； GEPIA2 database was used to analyze the correlation between THBS2 gene and the expression of tumor marker gene and other members of THBS gene family in MPM patients. Results：The expression of THBS2 gene in MPM tissues was significantly higher than that in normal pleural tissues and normal lung tissues. There were significant differences in THBS2 gene expression between N0 and N3， N1 and N3， N2 and N3 in MPM tissues. The high expression of THBS2 gene and sarcomatous and biphasic mixed tumors are independent risk factors for poor prognosis in patients with MPM. The expression of CDH11 was positively correlated with THBS2， while CALB2 and MSLN were negatively correlated with THBS2. In addition， the expression of THBS1， THBS3， THBS4 was positively correlated with that of THBS2. Conclusion：THBS2 is expressed differently in different types of cancer. Through the analysis of public database， it is found that the expression level of THBS2 gene is increased in human MPM tissue， and the expression of THBS2 is negatively correlated with the prognosis of patients. Therefore， THBS2 can be used as a potential marker for the preliminary diagnosis and prognosis of MPM.

Key words:

"> ">〔Key words">〕 malignant pleural mesothelioma">, thrombospondin-2">, data mining">, prognostic significance

CLC Number:

Li Bin, Li Sufen, Yang Na, Xiong Wei.

Expression and Significance of THBS2 Gene mRNA in Human Malignant Pleural Mesothelioma Based on Tumor Databases [J]. Journal of Dali University, 2022, 7(4): 26-33.

References

〔1〕 CHAPMAN A， MULRENNAN S， LADD B， et al. Population Based Epidemiology and Prognosis of Meso-thelioma in Leeds，UK〔J〕.Thorax，2008，63（5）：435-439.

〔2〕 MAO W， ZHANG X， GUO Z， et al. Association of Asbestos Exposure with Malignant Mesothelioma Inci-dence in Eastern China〔J〕.JAMA Oncol，2017，3（4）：562-564.

〔3〕王凯歌，李为民.恶性胸膜间皮瘤的研究和管理：英国胸科协会指南解读〔J〕. 中华肺部疾病杂志（电子版）， 2020，

13（2）：265-268.

〔4〕常浩，余宗艳，王启明，等.120例恶性胸膜间皮瘤的临床特征及诊断分析〔J〕.肿瘤，2020，40（3）：199-205.

〔5〕 MUTTI L， PEIKERT T， ROBINSON B W S， et al. Scientific Advances and New Frontiers in Mesothelioma Therapeutics〔J〕. J Thorac Oncol，2018，13（9）：1269-1283.

〔6〕徐雷，梁桐，夏雨，等. THBS2在常见肿瘤中临床意义的研究进展〔J〕. 老年医学研究， 2020，1（2）：54-57.

〔7〕 CALABRO N E， KRISTOFIK N J， KYRIAKIDES T R. Thrombospondin-2 and Extracellular Matrix Assembly〔J〕. Biochim et Biophys Acta， 2014， 1840（8）： 2396-2402.

〔8〕 BORNSTEIN P. Thrombospondins as Matricellular Modu-lators of Cell Function〔J〕. J Clin Invest，2001，107（8）：929-934.

〔9〕 STENINA O I， TOPOL E J， PLOW E F. Thrombospon-dins， Their Polymorphisms， and Cardiovascular Disease〔J〕. Arterioscler Thromb Vasc Biol，2007，27（9）：1886-1894.

〔10〕 GORDON G J， ROCKWELL G N， JENSEN R V， et al. Identification of Novel Candidate Oncogenes and Tumor Suppressors in Malignant Pleural Mesothelioma Using Large-Scale Transcriptional Profiling〔J〕. Am J Pathol， 2005，166（6）：1827-1840.

〔11〕 KIM M K， KIM H W， JANG M， et al. LOX Family and ZFPM2 as Novel Diagnostic Biomarkers for Malignant Pleural Mesothelioma〔J〕. Biomark Res，2020，8（1）：1-7.

〔12〕范冲冲，乔建瓯. 血小板反应蛋白2与肿瘤〔J〕. 国际肿瘤学杂志，2021，48（3）：164-166.

〔13〕 WENG T Y， WANG C Y， HUNG Y H， et al. Differential Expression Pattern of THBS1 and THBS2 in Lung Cancer： Clinical Outcome and a Systematic-Analysis of Microarray Databases〔J〕. PLoS One， 2016， 11（8）： e0161007.

〔14〕 KIM J， BAMLET W R， OBERG A L， et al. Detection of Early Pancreatic Ductal Adenocarcinoma with Thrombos-pondin-2 and CA19-9 Blood Markers〔J〕. Sci Transl Med，2017，9（398）：eaah5583.

〔15〕杨柳，罗黔. 胰腺癌组织高表达血小板反应蛋白2的预后意义及其对癌细胞增殖和迁移的影响〔J〕. 中国肿瘤生物治疗杂志， 2019，26（2）：206-212.

〔16〕 WANG X， ZHANG L， LI H， et al. THBS2 Is a Potential Prognostic Biomarker in Colorectal Cancer〔J〕. Sci Rep， 2016，6：33366.

〔17〕 SUN R， WU J， CHEN Y， et al. Down Regulation of Thrombospondin2 Predicts Poor Prognosis in Patients with Gastric Cancer〔J〕. Mol Cancer，2014，13：225.

〔18〕孙若川，李永翔. THBS2在胃癌组织中的表达与预后的关系〔J〕. 安徽医科大学学报， 2014，49（7）：995-999.

〔19〕吴湘光，魏文斐，梁莉，等. THBS2在宫颈癌中的表达及功能研究〔J〕. 现代妇产科进展，2017，26（12）：889-892.

〔20〕 KOCH M， HUSSEIN F， WOESTE A， et al. CD36-Mediated Activation of Endothelial Cell Apoptosis by an N-Terminal Recombinant Fragment of Thrombospondin-2 Inhibits Breast Cancer Growth and Metastasis in vivo〔J〕. Breast Cancer Res Treat，2011，128（2）：337-346.

〔21〕 SCHULTE J J， HUSAIN A N. Update on the Pathologic Diagnosis of Malignant Mesothelioma〔J〕. Transl Lung Cancer Res，2020，9（3）：917-923.

〔22〕 BEASLEY M B， GALATEAU-SALLE F， DACIC S. Pleural Mesothelioma Classification Update〔J〕. Virchows Arch，2021，478（1）：59-72.

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