Table of Content

15 April 2022, Volume 7 Issue 4

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In vitro Study on the Periplaneta americana Polypepticde PAE2 Reversing Multi-Drug Resistance of BEL-7402/5-FU Based on AMPK-Regulated Autophagy

Ou Ling, Li Cailin, Li Yinrui, Zhou Jie, Shan Wanxin, Wu Dingyu, Peng Fang

2022, 7(4):  1-7. 

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〔Abstract〕 Objective： To investigate the effects of the Periplaneta americana polypeptide PAE2 on the multi-drug resistance of BEL-7402/5-FU cells based on adenosine monophosphate-activited protein kinase（AMPK） regulation of autophagy. Methods： Human hepatocellular carcinoma sensitive cell line BEL-7402 and human hepatocellular carcinoma drug-resistant cell line BEL-7402/5-FU were used as the research objects. The action time and concentration of AICAR were determined by methyl thiazolyl tetrazolium（MTT） colorimetry assay. AMPK content in cells was determined by enzyme linked immunosorbent assay （ELISA）. Real-time polymerase chain reaction （real-time PCR） was used to detect AMPK mRNA expression level in cells. Real-time PCR was used to detect the mRNA expression levels of autophagy related protein 5（ATG5）， autophagy related protein 7（ATG7）， phosphoinositide-3-kinase class 3（PIK3C3）, Beclin 1，microtubuler-associated protein 1 light chain 3-Ⅱ（LC3-Ⅱ）， P62 and enhancer of Zeste homolog 2（EZH2）. The expression of LC3-Ⅱ was observed by laser scanning confocal microscope. The expression of P62 protein was detected by immunocytochemistry（ICC）. Results： Periplaneta americana polypeptide PAE2 can reduce the expression of AMPK and its mRNA， greatly reduce the expression of related autophagy proteins ATG5 mRNA， ATG7 mRNA， PIK3C3 mRNA， Beclin 1 mRNA， and LC3-Ⅱ mRNA（P<0.05）， and significantly increase the expression of P62 mRNA和EZH2 mRNA（P<0.05）， increase the expression of P62 protein（P<0.05）. Conclusion： Periplaneta americana polypeptide PAE2 can reverse the multi-drug resistance of BEL-7402/5-FU cells by inhibiting AMPK so as to inhibit autophagy.

Comparative Analysis of Liposoluble Constituents in the Larvae and Adults of Black Soldier Fly by GC-MS

Qing Chen, Chen Jinghu, Li Bolong, Liu Heng, Zhang Chenggui

2022, 7(4):  8-11. 

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〔Abstract〕 Objective： To analyze and compare the differences of liposoluble constituents of the larvae and adults of black soldier fly. Methods： The liposoluble constituents of the larvae and adults of black soldier fly were extracted by Soxhlet extraction method and determined by using GC-MS， the area normalization method was used to analyze the relative amounts of compounds between them. Results： Totally 47 peaks were detected from the larvae of black soldier fly and 22 chemical compounds of which were identified， accounting for 47.31% of the total content of liposoluble components； 21 peaks were detected from the adults of black soldier fly， and 16 chemical compounds were identified， accounting for 62.47% of the total content of liposoluble components. Conclusion： Hydrocarbons and lipid compounds are the characteristic components of the oil of black soldier fly. The adult contains more aliphatic acids， while the larvae contain a large number of olefin and phenolic compounds， which provide a certain reference for the resource utilization and development of black soldier fly.


Meta-Analysis of Cutaneous Adverse Reactions Induced by Dabrafenib Combined with Trametinib in the Treatment of Melanoma

Wang Ruihang, Liu Xiaobo, Liao Ｓi, Hua Peng

2022, 7(4):  12-20. 

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〔Abstract〕 Objective： To systematically evaluate the incidence of cutaneous adverse reactions induced by dabrafenib combined with trametinib in the treatment of melanoma. Methods： PubMed， EMbase， Cochrane Library， Web of Science， CNKI， VIP， Wanfang and China Biomedical Literature Database were searched by computer， and relevant published studies at home and abroad were collected. The retrieval period was from the establishment of the database to December 2020. Stata 15.1 software was used to calculate the incidence of cutaneous adverse reactions. RevMan 5.3 software was used to assess the relative risk（RR） for all grades of cutaneous adverse reactions. Results： A total of 10 studies involving 2 623 patients were included. The incidence of cutaneous adverse reactions of all grades in the treatment of melanoma with dabrafenib combined with trametinib were as follows：exanthem 22%［95%CI（0.19， 0.26）］， pruritus 10%［95%CI（0.08， 0.11）］， hyperkeratosis 7%［95%CI（0.04， 0.10）］， alopecia 5%［95%CI（0.04， 0.07）］， cutaneous papilloma 1%［95%CI（0.01， 0.03）］， acneiform dermatitis 10%［95%CI（0.06， 0.14）］， cutaneous squamous cell carcinoma 2%［95%CI（0.00， 0.04）］， dry skin 10% ［95%CI （0.08， 0.12）］ and erythema 11%［95%CI（0.09， 0.13）］. Compared with BRAF inhibitor alone， dabrafenib combined with trametinib reduced the incidence of pruritus， hyperkeratosis， alopecia， cutaneous papilloma， and cutaneous squamous cell carcinoma（P<0.05）. Conclusion： Exanthem is a common cutaneous adverse reaction in patients with melanoma treated with darafenib combined with trametinib. Compared with BRAF inhibitor alone， the combination drug significantly reduced the occurrence of cutaneous adverse reactions.

Effects of SGLT2i on Bone Metabolism in Nephrotic Syndrome Model Rats

Hong Wenjuan, Zou Jiulin, Yu Zhuorui, Cheng Jiamao, He Minhua

2022, 7(4):  21-25. 

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〔Abstract〕 Objective： To study the effect of sodium glucose cotransporter 2 inhibitor（SGLT2i） canagliflozin on bone metabolism in rats with nephrotic syndrome（NS）. Methods： There were 42 male SD rats in total， among which 6 rats were randomly selected as NG group， and the other 36 rats were injected with adriamycin through tail vein for modeling and were randomly divided into 6 groups， i.e.， MG group（model group）， PG group（prednisone group）， LCG group（low-dose canagliflozin group）， HCG group（high-dose canagliflozin group）， LPCG group（low-dose canagliflozin+prednisone group） and HPCG group（high-dose canagliflozin+prednisone group）， with 6 rats in each group. The rats were treated by regular gavage every morning for 6 weeks. At the end of treatment， 24 h-UTP， urinary calcium， urinary phosphorus， serum albumin（ALB）， blood calcium， blood phosphorus， alkaline phosphatase（ALP） and bone mineral density were measured. Results： Compared with NG group， ALP level increased in MG， PG， HCG and HPCG groups， and pelvic bone mineral density decreased in all drug treatment groups， PG and HPCG group caused the decrease of systemic bone mineral content， trunk and rib bone mineral density. Compared with MG group， 24h-UTP level decreased and ALB level increased in all drug treatment groups.Urinary calcium level increased in LPCG and HPCG groups. Serum phosphorus level increased in PG group. Conclusion：canagliflozin can effectively reduce urinary protein and increase ALB. So it can be used in the treatment of NS， but it can cause the decrease of pelvic bone mineral density. High-dose canagliflozin or its combination with prednisone can also cause abnormal calcium and phosphorus metabolism， increase ALP， expand the range of bone mineral density decline， and increase the risk of osteoporosis.

Expression and Significance of THBS2 Gene mRNA in Human Malignant Pleural Mesothelioma Based on Tumor Databases

Li Bin, Li Sufen, Yang Na, Xiong Wei

2022, 7(4):  26-33. 

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〔Abstract〕 Objective：To analyze the expression level and clinical significance of thrombospondin-2 （THBS2） gene mRNA in patients with malignant pleural mesothelioma （MPM） based on tumor databases. Methods： The difference of THBS2 mRNA expression between MPM and normal tissues was analyzed by Oncomine database； and the correlation between THBS2 mRNA expression and clinicopathological parameters of MPM patients was analyzed by TCGA database and UALCAN database. Kaplan- Meier survival model was constructed to analyze the prognostic effect of THBS2 gene expression on MPM patients； COX regression model was constructed to explore the independent risk factors affecting the prognosis of MPM patients； GEPIA2 database was used to analyze the correlation between THBS2 gene and the expression of tumor marker gene and other members of THBS gene family in MPM patients. Results：The expression of THBS2 gene in MPM tissues was significantly higher than that in normal pleural tissues and normal lung tissues. There were significant differences in THBS2 gene expression between N0 and N3， N1 and N3， N2 and N3 in MPM tissues. The high expression of THBS2 gene and sarcomatous and biphasic mixed tumors are independent risk factors for poor prognosis in patients with MPM. The expression of CDH11 was positively correlated with THBS2， while CALB2 and MSLN were negatively correlated with THBS2. In addition， the expression of THBS1， THBS3， THBS4 was positively correlated with that of THBS2. Conclusion：THBS2 is expressed differently in different types of cancer. Through the analysis of public database， it is found that the expression level of THBS2 gene is increased in human MPM tissue， and the expression of THBS2 is negatively correlated with the prognosis of patients. Therefore， THBS2 can be used as a potential marker for the preliminary diagnosis and prognosis of MPM.

Effects of the 7-Hydroxyisoflavone in the Proliferative Activity of HaCaT Cells and UVB-induced Photodamage

Duan Yi, Ni Changwen, Wang Minhua, Sun Sujiao, Huang Ling

2022, 7(4):  34-39. 

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〔Abstract〕 Objective： To investigate the effects of the 7-Hydroxyisoflavone on the proliferative activity of HaCaT cells and the UVB-induced photodamage， as well as its mechanism. Methods： The cell counting assay was used to detect the effects of different concentrations of 7-Hydroxyisoflavone on the proliferative activity of HaCaT cells. Next， the HaCat cells were pretreated with 200 μmol/L 7-Hydroxyisoflavone and were exposed to UVB（30 mJ/cm2）. Then， the production of reactive oxygen species（ROS） was detected by fluorescence method. The expressions of PARP1 and Caspase-8 were detected by Western blot. Results：The 7-Hydroxyisoflavone had the most obvious effect on the proliferative activity of HaCaT cells at the concentration of 200 μmol/L， and it could reduce the production of UVB-induced ROS. Besides， it could also inhibit the expressions of the PARP1 and Caspase-8.Conclusion：The 7-Hydroxyisoflavone can reduce photodamage caused by UVB and has a photoprotective effect on cells， which can be used to prevent skin damage caused by UVB.

Analysis of Risk Factors for Bone Cement Leakage during Percutaneous Kyphoplasty

Huang Zhong, Jia Ruipeng, Liu Yiheng, Zhang Chao, Liu Ke

2022, 7(4):  40-43. 

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〔Abstract〕 Objective： To investigate the risk factors and related preventive measures of bone cement leakage during percutaneous kyphoplasty （PKP） in the treatment of osteoporotic vertebral compression fracture（OVCF）. Methods： The case data of OVCF treated with PKP in the Department of Orthopedics， Ｈaikou Affiliated Hospital of Ｃentral South University Xiangya School of Medicine from December 2016 to December 2019 were retrospectively analyzed. The patients were divided into the leakage group and the non-leakage group according to whether the bone cement leakage occurred during the operation. The related indicators that may affect bone cement leakage were compared between the two groups， and the main risk factors for bone cement leakage were analyzed by Logistic regression. Results： Among the 107 patients， 18 patients had bone cement leakage， and the penetration rate was 16.82%.The comparative analysis of the basic data of the patients and Logistic regression analysis showed that age≥65 years old， bone cement injection volume ＞5 mL， and preoperative Cobb angle ＞24° were independent risk factors for bone cement leakage during PKP surgery.Conclusion： Age， the intraoperative bone cement injection volume and preoperative Cobb angle of PKP patients are independent risk factors for bone cement leakage， and bone cement injection volume is the most major risk factor.

Analysis of Influencing Factors of New Fractures after Osteoporotic Vertebral Compression Fracture Surgery

Luo Xinlei, Zhao Hui, Bai Tao, Xu Song, Zhang Xiongwen, Na Qiang

2022, 7(4):  44-46. 

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〔Abstract〕 Objective： To analyze the influencing factors of new fractures after osteoporotic vertebral compression fracture （OVCF）.Methods： A total of 93 OVCF patients （115 vertebrae） admitted to the Department of Orthopedics of the Sixth Affiliated Hospital of Kunming Medical University from January 2017 to January 2020 were selected as the research objects， which concluded 26 males （35 vertebrae） and 67 females（80 vertebrae）.All patients underwent vertebroplasty. Logistic regression model was used to analyze the influencing factors of postoperative new fractures. Results： Among the 31 patients with new fractures， 4 of who were male and 27 were female； 11 cases had adjacent vertebral body fractures and 33 cases had non-adjacent vertebral body fractures， and 2 cases with femoral neck fractures. Logistic analysis showed that gender， age， BMI， anti-osteoporosis treatment and the number of vertebral bodies operated were the influencing factors of new fractures， and the differences were statistically significant（P<0.05）.Conclusion：Female， age≥75 years， BMI ≥22 kg/m2， no anti-osteoporosis treatment and more than one vertebral body required surgery have a higher risk of new fractures.

Comparison of Curative Effects of MIS-TLIF and PLIF in the Treatment of Single-Segment Lumbar Degenerative Diseases

Xu Sanxiong, Zhang Jiyuan, Ma Xueying, Wei Guosong, Li Lin

2022, 7(4):  47-51. 

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〔Abstract〕 Objective： To retrospectively analyze and compare the efficacy of minimally invasive transforaminal lumbar interbody fusion （MIS-TLIF） and posterior lumbar interbody fusion（PLIF） in the treatment of single-segment lumbar degenerative diseases.Methods： A total of 60 patients with single-segment lumbar degenerative diseases who were treated in The First People's Hospital of Dali City from March 2018 to March 2020 were selected. The patients who underwent MIS-TLIF surgery were included in the experimental group while the patients who underwent PLIF surgery were classified as the control group， thus 30 cases in each group. And the operation time， intraoperative blood loss， intraoperative fluoroscopy time， postoperative drainage volume， time to get out of bed， and hospitalization time were recorded. All patients were followed up regularly， and the surgical efficacy was evaluated according to the visual analogue scale （VAS） and Oswestry disability index （ODI）. Results： There were no significant differences in gender， age， clinical diagnosis， lesion location， and preoperative VAS and ODI scores between the two groups（P>0.05）. Comparing the two groups， there was no significant difference in operation time and intraoperative fluoroscopy time between the two groups（P>0.05）.The amount of intraoperative blood loss， postoperative drainage， time to get out of bed， and hospitalization time in the experimental group were significantly less than those in the control group， and the differences were statistically significant （P<0.05）. The VAS and ODI scores of the experimental group before discharge and 3 months after the operation were better than those of the control group， which difference was statistically significant（P<0.05）. Conclusion： Compared with PLIF， MIS-TLIF has the advantages of less intraoperative blood loss， less trauma， and faster postoperative recovery.

Clinical Evaluation of Oblique Lateral Interbody Fusion in the Treatment of Elderly Patients with Lumbar Tuberculosis

Liu Siyuan, Rao Tao, Zhao Heng, Dou Jichen, Zhou Yi, Liu Qinghua, Cun Ｘinhua,

2022, 7(4):  52-56. 

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〔Abstract〕 Objective： To compare the short-term clinical effects of oblique lateral interbody fusion（OLIF） and posterior lumbar interbody fusion（PLIF） in the treatment of elderly patients with lumbar tuberculosis. Methods： A retrospective analysis of the case data of 42 patients with lumbar tuberculosis with intervertebral space lesions treated by orthopedic surgery in The Third People's Hospital of Kunming from October 2019 to January 2021.According to surgical methods， they were divided into OLIF group（n=15） and PLIF group（n=27）.The operation time， intraoperative blood loss， postoperative out-of-bed activity time， and complications were compared between the two groups. Results： There were no significant differences in operation time and complication rate between the two groups （P>0.05）.The OLIF group had better blood loss during operation and postoperative out-of-bad activity time than the PLIF group， and the differences were statistically significant（P<0.05）.At the last follow-up， no signs of tuberculosis recurrence were found in all patients， and no internal fixation device obstruction was found. Conclusion： The short-term effect of OLIF in the treatment of lumbar tuberculosis mainly with intervertebral space destruction is safe and effective. Compared with traditional PLIF， it has less intraoperative blood loss， less postoperative pain， less trauma， does not increase the operation time and complication rate， and meets the requirements of rapid recovery. Whether polyetheretherketone materials can be used safely in the treatment of spinal tuberculosis still needs further observation and research.

Analysis of the Treatment of Early Incision Deep Infection after Posterior Lumbar Internal Fixation and Fusion

Hu Jianhua, Hu Qi, Gao Guoxi, Lian Xingye, Yang Zhao, Zhang Chao

2022, 7(4):  57-59. 

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〔Abstract〕 Objective： To investigate the risk factors and treatment methods of early incision deep infection after posterior lumbar internal fixation and fusion. Methods： The case data of patients undergoing posterior lumbar internal fixation and fusion in the Department of Orthopedics of the First People's Hospital of Yunnan Province from January 2010 to December 2018 were retrospectively analyzed， and all patients were assessed for risk factors for infection（RFS）. The patients were divided into non-infection group（n=1 481） and infection group（n=11） according to whether or not patients have an infection. The patients in the infection group were treated with thorough debridement， closed irrigation and drainage， and antibiotics. Results： The difference in RFS between the two groups was statistically significant（P＜0.05）. In the infection group， 6 cases were cured after debridement， closed irrigation and drainage and antibiotic treatment， 1 case died of incision infection complicated with intracranial infection， and 4 cases were cured of infection after repeated debridement， closed irrigation and antibiotic treatment to remove internal fixator. Conclusion： The incidence of early incision infection after posterior lumbar internal fixation and fusion is low， and thorough debridement， closed irrigation and drainage and antibiotic therapy can cure infection； the internal fixator should be removed after thorough debridement for patients with long-term oral corticosteroids and RFS>3 points.

Analysis of Clinical Treatment of Lumbar Brucellosis

Gao Guoxi, Lian Xingye, Liu Weibing, Zhang Jun, Hu Jianhua, Lei Yunkun, Yang Zhao, Zhang Chao

2022, 7(4):  60-62. 

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〔Abstract〕 Objective： The lumbar Brucella infection is relatively rare and easy to be misdiagnosed. The treatment experience of lumbar brucellosis is summarized through case analysis. Methods： The case data of lumbar brucellosis in the First People's Hospital of Yunnan Province from January 2015 to January 2019 were retrospectively analyzed. Results： A total of 11 patients were included in the study. All patients received drug therapy and surgery. The preoperative visual analogue scale（VAS） score was（6.00±0.77） points， and the last follow-up VAS score was（0.64±0.50） points； The preoperative lumbar Oswestry disability index（ODI） score was （37.64±6.90） points， and the final follow-up ODI score was（13.73±4.05） points， and the differences were statistically significant（P<0.05）.Conclusion： Lumbar brucellosis can achieve good therapeutic effect through drug therapy and surgical treatment.

Clinical Application of Improved Preoperative Preparation Method for DAA Total Hip Arthroplasty

Peng Yunchuan, Luo Ting, Qi Lin, Ma Hongbing

2022, 7(4):  63-65. 

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〔Abstract〕 Objective： To evaluate the improved method of preoperative preparation for direct anterior approach（DAA） total hip arthroplasty so as to explore its clinical value. Methods：From January 2020 to November 2020， the clinical data of 54 patients who completed DAA primary total hip arthroplasty in the Department of Orthopedics of The Second People's Hospital of Chengdu were retrospectively analyzed. According to whether the modified method was used or not， the patients were divided into the modified group and the traditional group. The preoperative preparation time， operation time， disinfection area and Harris score were compared between the two groups. Results： There were significant differences in preoperative preparation time and disinfection area between the two groups（P<0.05）. The operation time of the two groups was similar， and the difference was not statistically significant（P>0.05）. There was no significant difference in preoperative Harris score between the two groups（P>0.05）. The postoperative Harris score in the modified group was higher than that in the traditional group， with a statistically significant difference（P<0.05）.Conclusion：After the improvement， DAA can reduce the disinfection area， shorten the operation preparation time as well as the duration of anesthesia and the risk of anesthesia. And it is conducive to the rapid recovery of the hip joint function of patients after surgery， which has clinical application value.

Observation on the Short-Term Effect of Enhanced Recovery after Surgery Concept Combined with Artificial Femoral Head Replacement in the Treatment of Elderly Intertrochanteric Fractures

Wang Hongping, Yang Xiaoqin, Tao Qifeng, Wang Mingyou, Lan Yuping

2022, 7(4):  66-70. 

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〔Abstract〕 Objective： To observe the clinical effect of enhanced recovery after surgery（ERAS） concept combined with artificial femoral head replacement in the treatment of elderly intertrochanteric fractures. Methods： The case data of 87 elderly patients with intertrochanteric fractures admitted to the Department of Orthopedics of Panzhihua Central Hospital from January 2018 to December 2020 were retrospectively analyzed. According to the treatment method， they were divided into an observation group（52 cases） and a control group（35 cases）. Both groups adopted the ERAS concept. The observation group was treated with artificial femoral head replacement， and the control group was treated with proximal femoral nail antirotation（PFNA）. Intraoperative blood loss， blood transfusion， operation time， incision length， complications and fatality in the early postoperative period and during follow-up， postoperative out-of-bed time， hospitalization time and satisfaction were evaluated in the two groups. Results： There were no significant differences in operation time， blood transfusion rate， complication rate and fatality rate between the two groups （P>0.05）.The patients in the observation group had more intraoperative blood loss and longer surgical incisions， but the time to get out of bed and hospitalization time were shorter than those of the control group， and the patient satisfaction was higher， and the differences were statistically significant（P＜0.05）. Conclusion： ERAS concept combined with artificial femoral head replacement for the treatment of elderly intertrochanteric fractures can improve the hip function of patients earlier， relieve hip pain， meet the needs of patients for early getting out of bed， improve early quality of life， shorten hospitalization time， and increase patient satisfaction with treatment. It is a safe and effective treatment.

Application of Enhanced Recovery after Surgery Concept in Perioperative Period of Total Knee Arthroplasty

Song Xunzhou, Wang Hongping, Lan Yuping

2022, 7(4):  71-74. 

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〔Abstract〕 Objective： To explore the application effect of enhanced recovery after surgery（ERAS） concept in perioperative period of unilateral total knee arthroplasty（TKA）. Methods： The case data of patients undergoing unilateral TKA in the Department of Orthopedics of Panzhihua Central Hospital from January 2017 to April 2021 were retrospectively analyzed. The 175 patients were divided into a control group（80 cases） and an experimental group（95 cases） by random number table method. The lengths of hospitalizations， the operation times， the times of ambulation， the intraoperative rehydration， the perioperative blood transfusion rates， the urinary catheter retention rates， the visual analogue scale （VAS） scores， the HSS knee scores and postoperative laboratory indexes were assessed between the two groups. Results： Compared with the control group， the length of hospitalization in the experimental group was significantly shortened， the amount of intraoperative rehydration was significantly reduced， the time for ambulation was significantly earlier， and the retention rate of urinary catheter and blood transfusion rates in the perioperative period were significantly reduced， and the differences were statistically significant（P<0.05）. The postoperative VAS scores of the experimental group were lower than those of the control group， and the postoperative HSS scores were higher than those of the control group， and the differences were statistically significant（P<0.05）. The hemoglobin and hematocrit of the experimental group were higher than those of the control group at the same period， and the C-reactive protein was lower than that of the control group at the same period， and the differences were statistically significant（P<0.05）. Conclusion： The application of ERAS concept in the perioperative period of TKA can effectively relieve the pain of patients， improve joint function， shorten the hospitalization time， and promote the recovery of patients， which is worthy of clinical application.

Analysis on the Quality of Life and Influencing Factors among Some Primary School Teachers in a Certain Area of Dali Bai Autonomous Prefecture

Feng Yipeng, Dou Can, Wang Xiaoping, Chen Hongyun, Li Jiangyan

2022, 7(4):  75-80. 

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〔Abstract〕 Objective： To investigate the quality of life and influencing factors of some primary school teachers in a certain area of Dali Bai Autonomous Prefecture. Methods： Cluster sampling was used to randomly select 10 primary schools in a certain area of Dali Bai Autonomous Prefecture， and questionnaires were carried out by using " questionnaire star " and some paper questionnaires. Multiple linear regression method was used to analyze the influencing factors of primary school teachers' quality of life. Results： The physical component summary（PCS） and mental component summary（MCS） scores of the quality of life of some primary school teachers in a certain area of Dali Bai Autonomous Prefecture were （42.86±8.36） and （46.31±10.79）， respectively. Multiple linear regression analysis showed that being ill（β=-7.90， t=-9.43， P<0.001）， high work pressure（β=-2.82， t=-3.86， P<0.001）， great work pressure（β=-2.97， t=-2.39， P=0.017）， 30-39 years old （β=-2.06， t=-2.12， P=0.034）， 40-49 years old （β=-3.56， t=-3.55， P＜0.001）， 50-59 years old（β=-5.40， t=-4.15， P<0.001）， obese（β=-3.81， t=-2.83， P=0.005）， very satisfied with the primary school teacher policy（β=3.87， t=2.39， P=0.017） were correlated with PCS. 3 000 yuan≤personal monthly income＜4 000 yuan（β=-6.69， t=-2.89， P=0.004）， 4 000 yuan≤personal monthly income＜5 000 yuan（β=-5.59， t=-2.59， P=0.010）， personal monthly income≥5 000 yuan（β=-5.36， t=-2.41， P=0.016）， being ill （β=-3.78， t=-3.33， P＜0.001）， high working pressure （β=-7.07， t=-7.27， P＜0.001） ， great work pressure （β=-8.66， t=-5.21， P<0.001）， patients with diabetes mellitus （β=9.96， t=3.40， P<0.001）， satisfied with the primary school teacher policy （β=4.48， t=2.23 ， P=0.026）， very satisfied with the primary school teacher policy （β=5.05， t=2.33， P=0.020）， 40-49 years old （β=3.55， t=2.34， P=0.019）， 50-59 years old （β= 4.45， t=2.28， P=0.023） were correlated with MCS. Conclusion： The PCS and MCS scores of quality of life of some primary school teachers in a certain area of Dali Bai Autonomous Prefecture were low. Primary school teachers who are ill， dissatisfied with the policy for primary school teachers， under heavy work pressure and obese should be focused on， and measures should be taken to improve the quality of life of primary school teachers.

Analysis of the Timeliness and Influencing Factors of Left-Behind Children's Medical Treatment in Nanchong Area

Jia Qinyao, Zhang Jianwu, Wen Jing, Chen Jianhua, Yu Chunlei, Yang Lixia

2022, 7(4):  81-84. 

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〔Abstract〕 Objective： To explore the timeliness and influencing factors of left-behind children's medical treatment in Nanchong area. Methods：From October 2020 to April 2021， 497 left-behind children and their guardians were selected in Nanchong City for investigation. The effects of various factors on the medical timeliness of left-behind children were evaluated by multivariate analysis. Results：A total of 497 questionnaires were issued in this survey， of which 490 valid questionnaires were retrieved， with an effective rate of 98.59%. A total of 19 people did not see a doctor within 2 weeks of illness， and the non-seeing rate was 3.88%. The results of multivariate Logistic regression analysis showed that the left-behind girls （OR=1.465， 95% CI：1.069~2.009）， the increase in the age of the main caregivers（OR=1.449， 95% CI：1.032~2.034）， and the distance from the nearest medical institution to home ≥3 km（OR=1.404，95% CI：1.099~1.793） were risk factors for the left-behind children in Nanchong area who did not seek medical treatment for illness within 2 weeks. The only child （OR=0.687，95% CI：0.497~0.950）， the increase in family monthly income （OR=0.662， 95% CI：0.460~0.951）， and participation in the new rural cooperative medical system（OR=0.707， 95% CI：0.562~0.889） were protective factors. Conclusion： In Nanchong area， the rate of the left-behind children who fell ill within 2 weeks without seeing a doctor was 3.88%， which is mainly affected by the gender of left-behind children， the age of main caregivers and the distance from the nearest medical institution to home. Relevant departments can intervene against the above factors to improve the medical immediacy of left-behind children in Nanchong Area.

Investigation on Language Developmental Delay of Children Aged 18-36 Months in Urban Area of Panzhihua City

Zou Yi, Li Peixuan, Tang Jun, Liu Ya, Yu Jun, Ye Mengya

2022, 7(4):  85-87. 

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〔Abstract〕 Objective： To investigate the language developmental delay of children aged 18-36 months in urban area of Panzhihua City. Methods： Stratified cluster sampling method was used to select children aged 18-36 months for questionnaire survey， language development screening， GESELL developmental diagnosis， physical examination， hearing test and other related examinations and diagnoses. Results： The detection rate of language retardation was 8.38%. Among them， the detection rate of boys was 11.69% while that of girls was 4.02%， and the ratio of detection rate of male to female was 3.8：1， and the difference was statistically significant（P< 0.01）.Only language area developmental delay accounted for 8.54%， overall developmental delay accounted for 83.54%， autism spectrum disorder accounted for 4.87%， cerebral palsy accounted for 0.61%， hearing impairment and cleft palate accounted for 1.22% respectively. There was a statistically significant difference in the detection rate of language developmental delay among children of different months（P<0.01）. The detection rate of children aged 18-24 months was the lowest at 5.20%； the detection rate of children aged 25-30 months was the highest， up to 12.53%. Conclusion： The incidence of language developmental delay in early childhood of boys is higher than that of girls， and it is most obvious in children aged 25 to 30 months. Overall global developmental delay accounts for the vast majority of language developmental delays.

Analysis of Clinical Application Effects of Disposable Blood Collection Needle in Arterial Blood Collection of Patients

Zhu Xingjie, Zhao Yanhong, Yang Quanxian, Chen Yonggang

2022, 7(4):  88-90. 

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〔Abstract〕 Objective： To explore the clinical application effects of disposable blood collection needle in arterial blood collection of patients so to provide basis for clinical operation. Methods： A total of 486 patients who collected arterial blood gas analysis specimens from Department of Tuberculosis of The Third People's Hospital of Kunming from January 2019 to January 2021 were selected as the research objects. According to the arterial blood collection method， they were divided into control group and observation group. The control group used the arterial blood collection device directly to collect blood， while the observation group used a disposable blood collection needle to puncture the artery successfully and then connected the arterial blood collection device to collect blood. The clinical application effects of the two methods were compared. Results： The success rate of one-time puncture， the accuracy rate of arterial blood sample collection and the overall satisfaction of patients in the observation group were significantly higher than those in the control group， and the incidence of blood collection complications and complaints were significantly lower than those in the control group， and the differences were statistically significant（P<0.05）. Conclusion： When collecting patient's arterial blood， using a disposable blood collection needle for puncture has a high success rate， which can completely avoid the situation that the blood collected is venous blood， thus is worthy of clinical promotion.

Qualitative Study on the Cognitive Status of Palliative Care in a Municipal Hospital of Kunming

Li Jiajia, Luo Yu, Huang Hongli, Li Hongjuan, Yang Limei, Xue Lian

2022, 7(4):  91-95. 

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〔Abstract〕 Objective： To explore the awareness， attitudes and demands of nurses in the Anning Care Unit for palliative care.Methods： From May 2019 to May 2020， 15 nurses in the nursing ward of a tertiary A hospital were conducted one-on-one in-depth interviews. The conversation materials were sorted out， and important information was extracted， repetitive views were identified and coded， and the coded views were assembled into themes. Results： Interviewees lack effective nursing methods in the aspects of psychological， social and spiritual problems of patients and their families. A comparative study with Swedish palliative care can effectively improve clinical application capabilities. Conclusion： The government should strengthen the social publicity of palliative care， carry out standardized and systematic teaching for medical students and medical staff. And improving the distribution of medical resources and support as well as guarantee are the decisive factors for accelerating its development.

Investigation and Analysis on the Healthy Needs of the Elderly in Pension Agencies in Dali Bai Autonomous Prefecture

Zhang Yanling, Zhang Ye, Liu Dan, Chen Jing, Duan Xiaorong, Li Kejin, Zhu Xiaoli

2022, 7(4):  96-100. 

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〔Abstract〕 Objective： To understand the current situation of the healthy needs of the elderly in pension agencies in Dali Bai Autonomous Prefecture， find out the shortcomings of the existing institutions， and put forward intervention strategies for the existing problems. Methods： 93 elderly people from three different pension agencies in Dali Bai Autonomous Prefecture were selected as the research objects. Through issuing the healthy demand questionnaire， the basic information， self-care ability， healthy status and their needs for daily life services， medical and health care services and spiritual civilization services were mastered， and the survey data were statistically analyzed. Results：The self-care ability of the subjects was generally not adequate， and 77.42% of the elderly could not take care of themselves completely；hypertension， cervical spine， waist and leg pain and diabetes and other basic diseases account for the highest proportion of diseases in the elderly， and the majority of elderly people suffer from chronic diseases at the same time；most elderly people choose to be taken to the hospital for treatment by their children when they get sick； the elderly hope to get complete daily life facilities and medical and health education. Conclusion：Basic diseases among the elderly in the pension agencies are prevalent， and recreational facilities are not enough. It is recommended that the institutions implement a combination of medical care and elderly care， strengthen the prevention and treatment of elderly diseases， improve facilities and services， improve the professional quality of nursing staff， and strengthen social attention to elderly care institutions.