Effect and Mechanism of Water-Soluble
Propolis on Ulcerative Colitis Exacerbated by Alcohol

Abstract

Abstract: Objective： To explore the protective effect of water-soluble propolis on easing the ulcerative colitis（UC） aggravated by alcohol and to explore its mechanism. Methods： 40 SPF male SD rats were randomly divided into the control group， the UC group， the UC+alcohol group， the UC+alcohol+propolis group and the UC+alcohol+mesalazine group. Except for the control group， the UC disease models were established by drinking dextran sodium sulfate（DSS） freely in other groups. Disease activity index（DAI） score， histological score（HS）， colon length measurement， colon quality measurement were performed on rats in each group， and the levels of the pro-inflammatory cytokines IL-6、 IL-17、TNF -?琢 and anti-inflammatory cytokine IL-10 in colon tissue were detected by ELISA. Results： UC rat model was successfully established by DSS. Compared with the UC group， the colon injury degree of the UC+alcohol group was enhanced， HS was significantly increased（P<0.01）， the levels of pro-inflammatory cytokines were significantly increased（P<0.01），and the level of anti-inflammatory cytokine was significantly reduced（P<0.01）； compared with the UC+alcohol group， the degree of colon injury in the UC+alcohol+propolis group and the UC+alcohol+mesalazine group were improved， HS was reduced（P<0.05）， the levels of pro-inflammatory cytokines were reduced， and the level of anti-inflammatory cytokine was increased（P<0.01）. Conclusion： Alcohol accelerates inflammatory responses in the gut and makes UC worse. Water-soluble propolis can reduce the level of pro-inflammatory cytokines in colon tissues， and increase the level of anti-inflammatory cytokines， thereby alleviating the injury of UC aggravated by alcohol and reducing the inflammation of the colon.

Key words: ulcerative colitis, alcohol, water-soluble propolis, cytokines

CLC Number:

R285.5

Zhang Ping, Fang Qiong, Zhou Hua.

Effect and Mechanism of Water-Soluble Propolis on Ulcerative Colitis Exacerbated by Alcohol [J]. Journal of Dali University, 2022, 7(8): 21-27.

References

〔1〕张晶娜，李超男，杜雯雯，等. 康复新液对溃疡性结肠炎大鼠肠道菌群的影响〔J〕.大理大学学报，2019，4（2）： 10-14.

〔2〕 SCHOULTZ I， KEITA ?魡 V. Cellular and Molecular Therapeutic Targets in Inflammatory Bowel Disease-Focusing on Intestinal Barrier Function〔J〕. Cells，2019， 8（2）：193.

〔3〕 SOLEIMANI D， MIRYAN M， TUTUNCHI H， et al. A Systematic Review of Preclinical Studies on the Efficacy of Propolis for the Treatment of Inflammatory Bowel Disease〔J〕. Phytother Res，2021，35（2）：701-710.

〔4〕 WANG J， ZHANG C L， GUO C M， et al. Chitosan Ameliorates DSS-Induced Ulcerative Colitis Mice by Enhancing Intestinal Barrier Function and Improving Microflora〔J〕. Int J Mol Sci，2019，20（22）：5751.

〔5〕 MA J M， YIN G H， LU Z B， et al. Casticin Prevents DSS Induced Ulcerative Colitis in Mice through Inhibitions of NF-κB Pathway and ROS Signaling〔J〕. Phytother Res， 2018，32（9）：1770-1783.

〔6〕 MARION-LETELLIER R， SAVOYE G， GHOSH S. IBD： In Food Ｗe Trust〔J〕. J Crohns Colitis， 2016， 10（11）： 1351-1361.

〔7〕 WHITE B A， RAMOS G P， KANE S. The Impact of Alcohol in Inflammatory Bowel Diseases〔J〕. Inflamm Bowel Dis，2022，28（3）：466-473.

〔8〕 WANG K， JIN X L， LI Q Q， et al. Propolis from Different Geographic Origins Decreases Intestinal Inflammation and Bacteroides SPP. Populations in a Model of DSS-Induced Colitis〔J〕. Mol Nutr Food Res，2018，62（17）：e1800080.

〔9〕 CHASSAING B， AITKEN J D， MALLESHAPPA M， et al. Dextran Sulfate Sodium（DSS）-Induced Colitis in Mice〔J〕. Curr Protoc Immunol，2014，104（1）： Unit 15.25.

〔10〕周华，张敏.水溶性蜂胶缓解大鼠溃疡性结肠炎作用机制研究〔J〕.中国食品卫生杂志，2020，32（6）：625-630.

〔11〕 BISHEHSARI F， MAGNO E， SWANSON G， et al. Alcohol and Gut-Derived Inflammation〔J〕. Alcohol Res， 2017，38（2）：163-171.

〔12〕 NEURATH M F. Cytokines in Inflammatory Bowel Disease〔J〕. Nat Rev Immunol，2014，14（5）：329-342.

〔13〕 LEE S H， KWON J E， CHO M L. Immunological Pathogenesis of Inflammatory Bowel Disease〔J〕. Intest Res，2018，16（1）：26-42.

〔14〕 BUNTE K， BEIKLER T. Th17 Cells and the IL-23/IL-17 Axis in the Pathogenesis of Periodontitis and Immune-Mediated Inflammatory Diseases〔J〕. Int J Mol Sci， 2019，

20（14）：3394.

〔15〕 KHOSRAVI A R， ALHEIDARY S， NIKAEIN D， et al. Aspergillus fumigatus Conidia Stimulate Lung Epithelial Cells （TC-1 JHU-1） to Produce IL-12，IFNγ，IL-13 and IL-17 Cytokines： Modulatory Effect of Propolis Extract〔J〕. J Mycol Med，2018，28（4）：594-598.

〔16〕 WANG S X， WANG J X， MA R， et al. IL-10 Enhances T Cell Survival and Is Associated with Faster Relapse in Patients with Inactive Ulcerative Colitis〔J〕.Mol Immunol，2020，121：92-98.

〔17〕 SKON-HEGG C， ZHANG J， WU X M， et al. LACC1 Regulates TNF and IL-17 in Mouse Models of Arthritis and Inflammation〔J〕. J Immunol，2019，202（1）：183-193.

〔18〕 OKAMOTO Y， TANAKA M， FUKUI T， et al. Brazilian Propolis Inhibits the Differentiation of Th17 Cells by Inhibition of Interleukin-6-Induced Phosphorylation of Signal Transducer and Activator of Transcription 3〔J〕. Immunopharmacol Immunotoxicol， 2012，34（5）：803-809.

〔19〕 WITTMANN DAYAGI T， WERNER L， PINSKER M， et al. Mucosal IL-10 and IL-10 Receptor Expression Patterns in Paediatric Patients with Ulcerative Colitis〔J〕. Int J Exp Pathol，2021，102（1）：4-10.

〔20〕 FANG D F， ZHU J F. Molecular Switches for Regulating the Differentiation of Inflammatory and IL-10-Producing Anti-Inflammatory T-Helper Cells〔J〕. Cell Mol Life Sci， 2020，77（2）：289-303.

〔21〕 KANG Y F， YANG G， ZHANG S M， et al. Goji Berry Modulates Gut Microbiota and Alleviates Colitis in IL-10-Deficient Mice〔J〕. Mol Nutr Food Res，2018，62（22）：e1800535.

〔22〕 BUENO-SILVA B， ROSALEN P L， ALENCAR S M， et al. Vestitol Drives LPS-Activated Macrophages into M2 Phenotype through Modulation of NF-κB Pathway〔J〕. Int Immunopharmacol，2020，82：106329.

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Effect and Mechanism of Water-Soluble Propolis on Ulcerative Colitis Exacerbated by Alcohol

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