Table of Content

15 August 2022, Volume 7 Issue 8

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Study on the Chemical Constituents of Chloroform Extract from Medicinal Insect Vespa auraria

Huang Yanmin, Pang Xiuqin, Xu Jinglei, Tang Songyuan, Li Yongjian, Yang Zhibin, Xiao Huai

2022, 7(8):  1-5. 

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 Objective： To study the chemical constituents of medicinal insect Vespa auraria. Methods： The monomer compounds were obtained by the methods of chromatographic separations and purification， and the structures of compounds were identified by carbon and hydrogen NMR data analysis combined with literature comparison. Results： Eleven compounds were isolated and identified from the chloroform extract of Vespa auraria， including five cyclic dipeptides： cyclo（Ala-Pro）（1）， cyclo（Pro-Pro）（2）， cyclo（Pro-Gly）（3）， cyclo（Pro-Tyr）（4）， cyclo（Thr-Pro）（5）， one acetylated amino acid： N-acetyl-isoleucine（6）， one organic amine： N-acetyl-4-（4-hydroxybenzene）ethylamine（7）， and three small molecule heterocyclic acids： nicotinic cacid（8）， 1-methyl-1，2，3，4-tetrahydro-carboline-3-carboxylic acid（9）， 3-indoleacetic acid（10）， and one benzodioxane compound：（2R，3S）-2-（3'，4'-dihydroxyphenyl）-3-acetylamino-7-（N-acetyl-2''-aminoethyl）-1，4-benzodioxane（11）. Conclusion： All the eleven compounds are isolated from this medicinal insect for the first time.

Simultaneous Determination of Seven Constituents Content in Quban Yangyan Capsule by HPLC

Kong Yan'e

2022, 7(8):  6-11. 

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Objective： To establish a method for the simultaneous determination of seven constituents content in Quban Yangyan capsule. Methods： HPLC method was adopted. The determination was performed on Yi Lite C18 column（4.6 mm×250 mm，5 μm）with mobile phase consisted of 0.1% phosphoric acid solution（A）-acetonitrile （B）（gradient elution） at the flow rate of 1.0 mL/min. The column temperature was set at 25 ℃，and the detection wavelength was set at 232 nm. The sample size was 10 μL. Results： Gallic acid，sodium Danshensu， geniposide， albiflorin，paeoniflorin，ferulic acid and salvianolic acid B showed good linear relationships within the ranges of 3.198-79.950 μg/mL（r=0.999 3）、3.798-94.950 μg/mL（r=0.999 9）、16.638-415.950 μg/mL（r=0.999 9）、6.772-169.300 μg/mL（r=0.999 5）、19.406-485.150 μg/mL（r=0.999 0）、1.612-40.300 μg/mL（r=1.000 0）、10.582-264.550 μg/mL（r=0.999 9），respectively. RSDs of precision，reproducibility， stability and added recovery tests were lower than 2.0％， which were qualified for quantitative testing. The average recoveries were 99.06%（RSD=0.73%），99.89%（RSD=0.54%），98.54%（RSD=0.90%），99.03%（RSD=0.74%），99.79%（RSD=0.56%），100.88%（RSD=0.54%） and 99.28%（RSD=0.48%）. Conclusions： This method is simple， specific， and has high precision， good separation and reproducibility， and can be used to determine the content of seven constituents in Quban Yangyan capsule.

Effect of Prescription Pre-Review System on the Qualified Rate of Outpatient and Emergency PPIs Prescriptions

Duan Xueyu, Liu Xiaobo, Chen Ruixiang, Shi Min

2022, 7(8):  12-15. 

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Objective： To investigate the effect of prescription pre-review system on the qualified rate of PPIs prescriptions in the First People's Hospital of Qujing. Methods：1 700 prescriptions of PPIs used in outpatient and emergency in this hospital from April to June 2019 without prescription pre-review system and 1 590 prescriptions of PPIs used in outpatient and emergency from April to June 2020 after the application of prescription pre-review system were selected for prescription review and analysis. Results：After the use of prescription pre-review system， the qualified rate of PPIs prescriptions in this hospital increased by 24.25%，and the types of irrational drug use in PPIs prescriptions decreased. Conclusion：The implementation of prescription pre-review system significantly reduces the irrational use of PPIs in outpatient and emergency in this hospital， greatly improves the qualified rate of PPIs prescriptions in outpatient and emergency departments of this hospital， and promotes the clinical rational drug use.

Application of Failure Mode and Effect Analysis in Prevention of Medication Errors/Medication Proximity Errors in the Hospital

Mao Panpan, Li Xingde, Zhang Yang, Liu Lu, Cai Manling , Xu Zhifeng, Song Cangsang

2022, 7(8):  16-20. 

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Objective： To investigate the application effect of failure mode and effect analysis（FMEA） in preventing medication errors/medication proximity errors in the First People's Hospital of Kunming. Methods： FMEA was used to evaluate and analyze the potential risk links in the medication process management of this hospital， and corresponding corrective measures were formulated according to the actual situation of the hospital. The risk priority number（RPN） of medication process management， medication errors/medication proximity errors rate and the doctor's order acceptance rate were compared before and after the implementation.Results： The RPN value after the implementation of FMEA was significantly lower than that before implementation（P<0.01）， the rate of medication errors/medication proximity errors decreased from 3.23‰ to 0.26‰（P<0.05）， the doctor's order acceptance rate increased from 99.60% to 99.83%. Conclusion： The application of FMEA can proactively identify potential risk in the management and safety of medication process， so as to optimize the medication process of the whole hospital， reduce the occurrence of medication errors/medication proximity errors， and ensure the medication safety of patients.

Effect and Mechanism of Water-Soluble Propolis on Ulcerative Colitis Exacerbated by Alcohol

Zhang Ping, Fang Qiong, Zhou Hua

2022, 7(8):  21-27. 

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Objective： To explore the protective effect of water-soluble propolis on easing the ulcerative colitis（UC） aggravated by alcohol and to explore its mechanism. Methods： 40 SPF male SD rats were randomly divided into the control group， the UC group， the UC+alcohol group， the UC+alcohol+propolis group and the UC+alcohol+mesalazine group. Except for the control group， the UC disease models were established by drinking dextran sodium sulfate（DSS） freely in other groups. Disease activity index（DAI） score， histological score（HS）， colon length measurement， colon quality measurement were performed on rats in each group， and the levels of the pro-inflammatory cytokines IL-6、 IL-17、TNF -?琢 and anti-inflammatory cytokine IL-10 in colon tissue were detected by ELISA. Results： UC rat model was successfully established by DSS. Compared with the UC group， the colon injury degree of the UC+alcohol group was enhanced， HS was significantly increased（P<0.01）， the levels of pro-inflammatory cytokines were significantly increased（P<0.01），and the level of anti-inflammatory cytokine was significantly reduced（P<0.01）； compared with the UC+alcohol group， the degree of colon injury in the UC+alcohol+propolis group and the UC+alcohol+mesalazine group were improved， HS was reduced（P<0.05）， the levels of pro-inflammatory cytokines were reduced， and the level of anti-inflammatory cytokine was increased（P<0.01）. Conclusion： Alcohol accelerates inflammatory responses in the gut and makes UC worse. Water-soluble propolis can reduce the level of pro-inflammatory cytokines in colon tissues， and increase the level of anti-inflammatory cytokines， thereby alleviating the injury of UC aggravated by alcohol and reducing the inflammation of the colon.

Expression and Clinical Significance of TTF-1 in Endometrial Carcinoma

Xu Chunhong, Dai Baoxiu, Pan Yun

2022, 7(8):  28-32. 

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Objective： To detect the expression of thyroid transcription factor-1（TTF-1） in normal endometrium， endometrial hyperplasia and endometrial carcinoma，and analyze the relationship between the TTF-1 expression and the clinical manifestation of carcinoma. Methods： The expression of TTF-1 was detected by immunohistochemistry in 11 specimens of regular endometrium， 17 specimens of endometrial hyperplasia， and 45 cases of endometrial carcinoma， and statistical analysis was performed combined with clinical data. Results：The positive rates of TTF-1 in normal endometrium， endometrial hyperplasia and endometrial carcinoma were 45%， 35% and 16%， respectively. Compared with normal endometrium， the positive rate of TTF-1 in endometrial carcinoma was lower， and the difference was statistically significant（P<0.05）.There was no significant difference in the expression of TTF-1 between type Ⅰ and type Ⅱ endometrial carcinoma. Compared with highly-differentiated endometrial carcinoma， TTF-1 expression was significantly up-regulated in moderately- and poorly- differentiated endometrial carcinoma（P<0.05）. The expression of TTF-1 in endometrial carcinoma was not correlated with the patient's nationality， age， clinicopathologic stage， depth of tumor invasion， and metastasis. Conclusion： TTF-1 may be involved in the occurrence and development of some endometrial carcinoma.

Detection of Common Pathogenic Bacterium in the Ghee Made by Ethnic Minority Farmers in an Area of Yunnan

Chen Zhaofang, Cao Xiaolan, Yu Wenjing, Du Juan, Wu Youcong

2022, 7(8):  33-36. 

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Objective： To investigate the microbiological indexes in the ghee made by ethnic minority farmers in an area of Yunnan， and to provide reference for the food safety of the ghee. Methods： 35 ghee samples were collected. The total number of bacteria and molds/yeast in the samples were detected after a series of dilution， and the food-borne pathogens were identified according to colony characteristics， morphological staining and biochemical reaction. Results： Bacteria were detected in all the 35 samples（100.0% detection rate）， the logarithm value of CFU（lgCFU） in the 35 samples ranged from 4.08 to 5.31 per gram， and the total number of bacteria in 26 ghee samples（74.3% exceeding standard rate） exceeded the standard number. Molds/yeasts were detected in 31 samples（88.6% detection rate）， with lgCFU value of 2.00-4.98 per gram， and bacteria in these 31 samples all exceeded the standard in CFU counting. Additionally， intestinal pathogens were detected in 4 species（11.4% detection rate）； 3 strains were suspected as Shigella species， and 1 as Proteus species. Staphylococcus aureus and Escherichia coli were not detected in the samples. Conclusion： The sanitary microbiological index of the ghee in this area is worrying. The mold contamination is serious， and intestinal pathogens are isolated from some samples.

Study on Subculture Methods of Helicobacter pylori Sydney-Standard Strain

Fan Yujuan, Li Fuxing, Wang Li, Cheng Mingjing, Xiao Min, Xiong Miaomiao, Zhao Weidong

2022, 7(8):  37-40. 

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Objective： To compare the growth rate of Helicobacter pylori（H. pylori） Sydney-standard strain（SS1 strain） by different culture methods， and to find a simple and fast culture method for H. pylori. Methods： The SS1 strain was cultured by four methods. Brain-heart infiltration fluid and bacterial fluid were added to Columbia blood plate， and the bacterial fluid was evenly spread with a disposable inoculating loop. Method 1 was to put the Columbia blood plate coated with bacterial liquid into a sealed anaerobic bag equipped with an anaerobic gas production bag. Method 2 was to place the Columbia blood plate coated with the bacterial solution in a sealed culture jar containing deionized water and an anaerobic gas production bag. Method 3 was based on method 2， in which a candle was lit once in the sealed culture jar. Method 4 was based on method 3， in which a candle was lit in the sealed culture jar for many times. The four methods were all completed in a CO2 incubator， and the H. pylori strains were identified by observing colony morphology and Gram staining， and detected by mass spectrometry. Results：H. pylori strains were successfully cultivated by the four methods. When the number of colonies reached the same level， Method 4 takes significantly less time than the other three methods， and the differences were statistically significant （P<0.05）. Conclusion： A simple and inexpensive method of H. pylori subculture has been found， which has strong application prospects and is worthy of promotion.

Bioinformatics Analysis of the Protein Encoded by the MAV_2927 Gene of Mycobacterium avium

Li Xiangfang, Ding Shoupeng, Gao Jinghua, Wu Lixian

2022, 7(8):  41-45. 

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Objective： Ｔo analyze the protein encoded by the pathogenic gene MAV_2927 of Mycobacterium avium（MAV） by using bioinformatics. Methods： The gene and amino acid sequence of the protein encoded by the MAV_2927 gene were obtained from NCBI；Protparam tool， SOPMA， SWISS-MODEL were applied to analyze the physical and chemical properties and secondary and tertiary structure of proteins；PortScale， SignalIP 4.0 Server， TMHMM Server 2.0 were used to analyze hydrophilicity and hydrophobicity， signal peptide， and transmembrane region. Results：The protein encoded by the MAV_2927 gene contains 43 amino acids， has a relative molecular weight of 4.213 kDa， an isoelectric point of 4.72， and a molecular formula of C183H284N48O62S2. Conclusion：The protein encoded by MAV_2927 contains multiple T and B cell epitopes， indicating that it may be a potential pathogenic factor related to the host interaction during the infection process， and can provide a basis for studying the pathogenicity of MAC and clinical treatment.

Research Progress on the Regulation Mechanism of Transcription of Mammalian Mitochondrial DNA

Xiong Wei, Liu Ruai, Li Bin, Zhang Bensi, Jiang Bei, Yu Min

2022, 7(8):  46-54. 

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Mitochondria are the principal site of ATP generation， and are involved in the regulation of apoptosis， calcium homeostasis， and innate immune response. The role of mitochondiral dysfunction in the occurrence and developmant of many huamn diseases has received increasing attention， which has promoted the biochemical study on the transcription process and regulation mechanism of mitochondria. The expression and regulation of the mitochondrial gene is very unique because it is regulated by both mitochondrial DNA （mtDNA） and nuclear genome. This essay reviews the research progress that has been done on the structure， transcriptional process and regulatory mechanism of mammalian mtDNA， and focuses on describing a variety of direct and indirect factors influencing mtDNA transcription. The hot issues relevant to its medical research will be analyzed and prospected.

The Construction and Application of Formative Evaluation System in Experimental Teaching of Basic Medicine

Qin Yan, Hu Yarong, Su Juan, Lai Mingming, Liu Yunchun, Yang Wenjuan

2022, 7(8):  55-57. 

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 Formative evaluation has become the major concerns of current medical education reform. During the course of experimental teaching of basic medicine， it's necessary to construct and optimize a formative evaluation index system， and put it into practice， in order to solve some problems caused by the traditional evaluation methods. Through a scientific， comprehensive and effective evaluation system， the mutual communication between teachers and students in teaching-learning process can be promoted， teaching and learning tragedies can be improved， and the quality of experimental courses can be enhanced.

Clinical Application of Two-Step Stratification Method Laparoscopic Left Hemihepatectomy

Xu Anshu, Hou Yong, Sun Yong, Yang Xiaobin, Zhou Lihua, Fu Chaochun

2022, 7(8):  58-61. 

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Objective： To observe the effect of two-step stratification method in laparoscopic left hemihepatectomy. Methods： Clinical data of 77 patients who underwent laparoscopic left hemihepatectomy in Qujing Hospital Affiliated to Kunming Medical University from May 2015 to December 2019 were analyzed retrospectively. According to the operation method， the patients were divided into the control group and the observation group. The illness index and data of the two groups of patients were compared， including the operation time， intraoperative bleeding， abdominal drainage time， anal exhaust time， hospital stay， serum alanine aminotransferase （ALT）， aspartate aminotransferase （AST） levels and complications. Results： Compared with the control group， the operation time of the observation group was significantly shortened， the amount of intraoperative bleeding was significantly reduced， and the levels of serum ALT and AST were significantly reduced after operation， and the differences were statistically significant （P<0.05）. There were no significant differences in the total incidence of complications and tumor recurrence rate during the follow-up period after operation between the two groups （P>0.05）. Conclusion： The application of two-step stratification method in laparoscopic left hemihepatectomy can achieve the same therapeutic effect as traditional hemihepatectomy. It can reduce the operation time and the amount of bleeding without increasing the incidence of complications and tumor recurrence. It is worthy of clinical application.

Clinical Analysis of 13 Pregnant Woman Complicated with Cerebral Venous Sinus Thrombosis

Wu Zeyu , Cen Jianchang , Ouyang Chao , Li Zeqi , Xie Renhua, Gao Hong , Su Ping

2022, 7(8):  62-65. 

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Objective： To analyze the data of the patients with cerebral venous sinus thrombosis（CVST） in pregnancy and puerperium， and to improve the understanding of pregnancies complicated by CVST. Methods：We reviewed and analyzed the records of the pregnant patients who also had a diagnosis of CVST in Ganmei Hospital Affiliated to Kunming Medical University from January 2013 to January 2019， including their general data， clinical manifestations， laboratory and imaging findings， treatment methods and prognosis. Results： Among the 13 patients， 10 were in puerperium， 8 had headache， 5 had elevated D-dimer in laboratory examination， and 11 had abnormalities by CT. All patients were clearly diagnosed by magnetic resonance imaging and magnetic resonance venography. The imaging results showed that 9 cases involved superior sagittal sinus and 8 cases involved transverse sinus， including 8 cases with more than 2 venous sinuses. 9 patients had a good prognosis， and 4 of the 5 surgery patientshad a good prognosis. Conclusion： CVST in pregnancies is more likely to occur in the puerperium. Headache is the most common clinical symptom. The possibility of CVST cannot be ruled out if there is no abnormality in D-dimer. The superior sagittal and transverse sinuses were the most commonly affected cerebral sinuses in the patients with CVST. So， the early diagnosis and selection of appropriate treatment methods can help most patients achieve a better prognosis.

Clinical Observation of Preoperative Sedation with Esketamine-Dexmedetomidine Combination or Esketamine-Midazolam Combination

Sun Dongyan, Jiang Xueli, Liu Qinglan, Yang Ruchen, Yang Yongyan, Zhao Xingmei

2022, 7(8):  66-70. 

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Objective： To observe the clinical efficacy of esketamine combined with intranasal dexmedetomidine or oral midazolam for preoperative sedation. Methods： A total of 120 children with groin surgery in the Department of Pediatrics of the Children's Hospital of Dali Bai Autonomous Prefecture were selected and randomly divided into 3 groups. The control group received oral doses of 0.3 mL/kg of rock sugar snow pear juice before operation； intranasal group received esketamine 0.5 mg/kg + intranasal dexmedetomidine 2 μg/kg）； oral group received esketamine 1.5 mg/kg + oral midazolam 0.5 mg/kg. Preoperative sedative effects， vital signs， drug acceptance satisfaction， the ease of child-parent separation， propofol-induced acceptance and post-wake agitation were observed before and after administration. Results： Compared with the control group， the parent-child separation score， the propofol acceptance score and the agitation score during recovery were significantly different between intranasal group and oral group （P<0.05）. The drug acceptance satisfaction score of intranasal group was significantly lower than those of the other two groups， and the difference was statistically significant（P<0.01）. During 10 to 30 minutes after administration， the sedation conditions of  oral and  intranasal groups were significantly different from those before administration（P<0.01）. There was no respiratory depression in the three groups during the monitoring time， and the vital signs were stable. There were no statistical differences in the incidence of adverse reactions before and after the operation among the three groups（P>0.05）. Conclusion：Esketamine combined with intranasal dexmedetomidine or oral midazolam is effective in sedation， making the perioperative period safer and more comfortable for children.

Application Value of Contrast-Enhanced Ultrasonography in the Diagnosis of Benign and Malignant Breast Tumors

Liu Xiaojun, Luo Chunxia, Yang Yingmei

2022, 7(8):  71-74. 

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Objective： To analyze the application value of contrast-enhanced ultrasonography in the diagnosis of benign and malignant breast tumors. Methods： A total of 246 patients with breast tumor（256 breast nodules） admitted to Baoshan People's Hospital from January 2018 to October 2020 were selected as the research objects. The results of contrast-enhanced ultrasonography and conventional ultrasound were evaluated based on postoperative pathological results. Results： 256 breast nodules were diagnosed by postoperative pathology， including 103 malignant tumors and 153 benign tumors. Contrast-enhanced ultrasonography correctly diagnosed 90 malignant tumors and 136 benign tumors. Conventional ultrasound correctly diagnosed 72 malignant tumors and 119 benign tumors. The sensitivity， specificity and accuracy of contrast-enhanced ultrasound in the diagnosis of benign and malignant breast tumors were higher than those of conventional ultrasound. Conclusion： Compared with conventional ultrasound， contrast-enhanced ultrasonography has higher sensitivity and accuracy in the diagnosis of benign and malignant breast tumors， and is worthy of promotion.

Clinical Significance of Emergency Blood Test in the Diagnosis of Wild Mushroom Poisoning

Yao Xiyou, Wang Jin, Zhan Xueliang, Gao Xiang, Xu Wenbo, Feng Lei

2022, 7(8):  75-78. 

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Objective： To analyze the results of emergency blood test in patients with wild mushroom poisoning， and to explore the clinical significance of emergency blood test for the diagnosis of patients with wild mushroom poisoning. Methods：This study included 50 suspected cases of patients who were initially diagnosed with wild mushroom poisoning in the People's Hospital of Yuxi Ｃity from June 1 to August 31， 2020（the group of suspected cases with wild mushroom poisoning）， and 50 healthy physical examination people during the same period（the healthy examination group）. The changes of routine blood examination， coagulation function， electrolytes， liver function and cardiac function were compared between the two groups. Results： Compared with the healthy examination group， the white blood cell count， neutrophil count and neutrophil percentage in the group of suspected cases with wild mushroom poisoning increased， while the lymphocyte count， lymphocyte percentage and monocyte percentage decreased. So the differences were statistically significant（P<0.05）. The levels of fibrin degradation product and D-dimer in the group of suspected cases with wild mushroom poisoning increased， and the difference was statistically significant（P<0.05）. The concentrations of bicarbonate ion and myoglobin decreased in the vast majority of patients with wild mushroom poisoning. Conclusion： The changes of hemogram in routine blood test， the increase of fibrin degradation product and D-dimer levels， and the decrease of bicarbonate ion and myoglobin concentrations can provide support for the diagnosis of mild wild mushroom poisoning.

Analysis of Distribution and Antibiotic Resistance of Mycoplasma pneumoniae Infection in a Hospital in Dali City from 2015 to 2020

Jia Han, Xu Jiaqi, Li Fuxing, Hu Shenghe

2022, 7(8):  79-82. 

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Objective： To analyze the clinical distribution characteristics and antibiotic resistance changes of Mycoplasma pneumoniae infection in the First Affiliated Hospital of Dali University from 2015 to 2020， and to provide reference for clinical medication. Methods：Expectorated or aspirated sputum samples in the First Affiliated Hospital of Dali University from 2015 to 2020 were isolated and cultured， strains in them were detected， and antibiotics susceptibility was applied for all isolated bacteria. And then the data were statistically analyzed. Results： A total of 185 strains of Mycoplasma pneumoniae were isolated， and the annual distribution showed a downward trend year by year. Patients mainly came from the Department of Respiratory Medicine and the Department of Infectious Diseases. The age mainly ranged from 41 to 80. The antibiotics susceptibility results showed that there was a statistically significant difference in the overall resistance rates of Mycoplasma pneumoniae to minocycline， acetylspiramycin， ciprofloxacin and clindamycin in this hospital from 2015 to 2020（P<0.05）. Conclusion： The resistance trend of Mycoplasma pneumoniae to antibiotics is stable from 2015 to 2020， and the dynamic monitoring of resistance should be strengthened to guide the rational use of antibiotics in clinical practice.

Analysis of a Prevalence Survey on Nosocomial Infections in the ICU of a Tertiary Hospital from 2015 to 2019

Duan Liping

2022, 7(8):  83-86. 

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Objective： To investigate the current situation and trend of nosocomial infection in the Intensive Care Unit（ICU） of a tertiary hospital， to implement the infection prevention and control measures appropriately， and to further improve and strengthen the prevention and control of nosocomial infection. Methods： The study collected data of all ICU patients with nosocomial infection symptoms from 2015 to 2019 in this hospital， and statistically analyzed the prevalence of nosocomial infections in ICU and the prevalence of nosocomial infection caused by common ICU equipment. Results： From 2015 to 2019， the average nosocomial infection rate in the ICU of the hospital was 6.40%， and the average prevalence of nosocomial infections was 7.32%. The prevalence of nosocomial infection reached the highest in 2016 and the lowest in 2019. There were significant differences in the rate of ventilator-associated pneumonia and central venous catheter infection among different years（P<0.05）. The rate of catheter-related urinary tract infection was relatively stable， and there was no significant difference between different years（P>0.05）. The infection rate of ventilator-associated pneumonia（6.24‰） was higher than that of central venous catheter infection（2.00‰） and catheter-related urinary tract infection （1.04‰）. Conclusion： The infection control situation in the ICU of this hospital is generally good. With the continuous improvement of nosocomial infection prevention and control， the prevalence of nosocomial infection shows a downward trend. In the future work， the prevention and control of nosocomial infection should be further strengthened， the respiratory tract infection should be the focus of prevention and control， the principle of nosocomial infection monitoring should be implemented， in order to reduce the nosocomial infection rate.

Analysis of Care Needs of Diabetic Patients and Influencing Factors in Dali Bai Autonomous Prefecture

Wang Shuang, Xu Wenwen, Tang Jun

2022, 7(8):  87-91. 

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Objective： To investigate the current situation of diabetic patients' needs for care in Dali Bai Autonomous Prefecture， and analyze the factors influencing their care needs. Methods： Convenience sampling method was used to select 200 patients with diabetes mellitus from endocrinology department of two general hospitals in Dali Bai Autonomous Prefecture， and the general information questionnaire and the care needs questionnaire for diabetic patients were used for investigation. Results： The total care demand score of diabetic patients in Dali Bai Autonomous Prefecture was （3.40±0.53） points. The major factors significantly influencing the nursing demand of diabetic patients include physical condition， duration of diabetes， place of residence， household monthly income and diabetic complications. Conclusion： The total needs of diabetic patients for care in Dali Bai Autonomous Prefecture is at a medium level. Attention should be paid to the care-oriented self-management education of diabetic patients， so as to improve their quality of life.

The Relationship between Academic Procrastination and Sleep Quality in Vocational Nursing Students： The Mediating Effect of Self-Control

Duan Min, Cheng Can, Lin Bo

2022, 7(8):  92-96. 

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Objective： To explore the mediating effect of self-control on the relationship between academic procrastination and sleep quality. Methods： A questionnaire survey was carried out among the vocational nursing students in West Anhui Health Vocational College by using the General Information Survey Scale， the Academic Procrastination Behavior Assessment Scale， the Pittsburgh Sleep Quality Index Scale and the Self-Control Assessment Scale. Results： The total score of academic procrastination behavior of 476 vocational nursing students was （141.53±19.68） points， the total score of sleep quality was （13.59±3.41） points， and the total score of self-control was （61.29±10.71） points. There was a positive correlation between academic procrastination and sleep quality scores in vocational nursing students， and a negative correlation between self-control scores and academic procrastination and sleep quality scores. Self-control had a mediating effect value of 41.42% in academic procrastination and sleep quality. Conclusion： The self-control ability of vocational nursing students significantly affects sleep quality through academic procrastination. It is suggested that college educators should actively improve teaching and life management to improve the physical and mental health of nursing students in higher vocational colleges.

Application of Curriculum Ideological and Political Concepts in the Teaching of Surgical Nursing Practice in Higher Vocational Colleges

Wang Dan, Zhou Bo, Yu Hui, Zhang Yanzhen

2022, 7(8):  97-100. 

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Objective：To explore the application effect of curriculum ideological and political concepts in the teaching of surgical nursing practice in higher vocational colleges. Methods： The lottery method was applied to divide the four classes of the higher vocational nursing major of Huangshan Vocational and Technical College into the control group and the experimental group. The experimental group adopted the blended learning based on the concept of curriculum ideology and politics， while the control group adopted the traditional teaching method. Before and after the implementation of the study， the nursing professional identity of the students in the two groups was evaluated， and the operation assessment scores and the teaching satisfaction of the practical training courses were compared between the two groups after teaching. Results： Before the implementation of the study， there was no significant difference in the total score and the scores of each dimension of nursing professional identity between the two groups（P>0.05）. After the implementation of the study， the students in the experimental group had higher scores in operation assessment， teaching satisfaction， and nursing professional identity than those in the control group， and the differences were statistically significant （P<0.05）. Conclusion： The application of blended teaching under the concept of curriculum ideology and politics in surgical nursing practice teaching for nursing students in higher vocational colleges can stimulate students' interest in learning， improve students' identity with nursing profession， and enhance teaching effects， which is worthy of promotion and application.