Abstract: Objective: To explore the expression of PRDX2 in sperm of asthenospermia individuals with overweight and its correlation
with sperm motility. Methods: A total of 101 semen samples from routine semen analysis at the reproductive medicine department of
the First Affiliated Hospital of Dali University were collected. Samples were grouped into a normal group （60 samples） with normal
body mass and sperm vitality and an overweight asthenospermia group （41 samples） with overweight and asthenospermia. Sperm
routine parameters were measured using a sperm automatic analyzer, and the localization and expression of PRDX2 in sperm from the
two groups were detected by indirect immunofluorescence. Correlation analysis was performed using relevant software to assess the
relationship between PRDX2 expression changes and semen parameters. Results: Indirect immunofluorescence showed that PRDX2
was mainly localized in the midpiece mitochondria of human sperm. The fluorescence expression of PRDX2 in sperm from the
overweight asthenospermia group was significantly lower than that in the normal group. PRDX2 fluorescence expression was negatively
correlated with body mass index （r=-0.857, P=0.002） and positively correlated with progressive motility percentage （r=0.827, P=
0.003）. Conclusion: The expression of PRDX2 in sperm is decreased in overweight individuals with asthenospermia, and its
expression is positively correlated with sperm motility.

Key words: asthenospermia, PRDX2, overweight, sperm motility