Changes in Color and Chemical Components during the Aging Process of Artemisia argyi Leaves

doi:10. 3969 / j. issn. 2096-2266. 2025. 08. 001

Abstract

Abstract: Objective: To investigate the dynamic changes in color, volatile and non-volatile components of Artemisia argyi leaves
during natural aging, and clarify the mechanism of chemical component transformation, providing a scientific basis for quality
evaluation and optimization of the aging process. Methods: A. argyi leaves of different aging years were used as the research object.
The color change was characterized by measuring the color parameters using a colorimeter. The volatile oil of A. argyi leaves was
extracted by steam distillation and the yield was calculated.Gas chromatogram-mass spectrometry （GC-MS） combined with orthogonal
partial least squares discriminant analysis （OPLS-DA） was applied to analyze the composition and differences of volatile components.
High-performance liquid chromatography （HPLC） was used to quantify five non-volatile components, including neochlorogenic acid
and chlorogenic acid. Results: With the prolongation of aging time, the color of A. argyi leaves gradually shifted from green to
yellowish-brown, and the volatile oil yield significantly decreased. GC-MS identified 92 volatile components, among which alkenes
and alcohols were the major components. OPLS-DA screened out 20 differential components, with contents of borneol, methyl eugenol,
p-cymen-8-ol, dihydrocarvone, and α-thujone increasing significantly. For non-volatile components, phenolic acids degraded faster
than flavonoids. Conclusion: The aging of A. argyi leaves is driven by the synergistic effects of volatile component transformation, nonvolatile
component degradation, and physicochemical changes. The formation of compounds like borneol reveals the complexity of
chemical conversion, while color changes serve as a visual indicator of aging progression. These findings provide a scientific basis for
establishing a quality evaluation system and optimizing the aging process, and offer a modern scientific interpretation of the traditional
concept "the longer the aging, the better the efficacy".

Key words: Artemisia argyi leaves, aging, volatile oil, chemical components, color change

CLC Number:

R284.1

Yang Dandan, Huang Haozhou, , Bao Xiaoming, Yang Ming, Zhang Dingkun, . Changes in Color and Chemical Components during the Aging Process of Artemisia argyi Leaves[J]. Journal of Dali University, 2025, 10(8): 1-10.

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References

〔1〕梅全喜. 蕲艾的研究与应用〔M〕. 北京：中国中医药出
版社，2020：1-342.
〔2〕 LIU Y，HE Y N，WANG F，et al. From Longevity Grass to
Contemporary Soft Gold：Explore the Chemical Constitu⁃
ents，Pharmacology，and Toxicology of Artemisia argyi H.
LÉV. & Vaniot Essential Oil〔J〕. J Ethnopharmacol，2021，

279：114404.

〔3〕郭秀丽，李水清，刘艳菊. 基于艾叶“陈久者良”的习用历
史对现行质量标准的分析与思考〔J〕. 世界中医药，
2022，17（9）：1208-1213.
〔4〕宁二娟，李晓，王伟，等. 不同陈化年份南阳艾叶挥发油
成分测定及抗氧化活性研究〔J〕. 中药材，2024（6）：
1415-1419.
〔5〕高鹏，陈利军，王喆之，等. 艾叶及其燃烧产物的化学成
分研究进展〔J〕. 安徽农业科学，2023，51（24）：19-23.
〔6〕夏佳璇. 艾叶酚类成分的质量控制与药代动力学研
究〔D〕. 武汉：湖北中医药大学，2020.
〔7〕刘昱，胡琦，贺亚男，等. 基于谱效关联分析挖掘艾叶挥
发油抑菌的质量标志物〔J〕. 天然产物研究与开发，
2023，35（6）：1017-1026.
〔8〕李皓翔，吴梦奇，范卫锋，等. 艾叶挥发性成分与非挥发
性成分指纹图谱分析〔J〕. 中国药师，2022，25（11）：
1952-1957.
〔9〕 KIM S，KIM S，SHIM I，et al. Drying Operation Effects on
the Pigments and Phytochemical Properties of Rose Culti⁃
vars〔J〕. J AOAC Int，2021，104（4）：1148-1154.
〔10〕张平辉. 高品质合成冰片及关键中间体的合成与表
征〔D〕. 北京：中国林业科学研究院，2014.
〔11〕戴瑞贤，黄宝康，刘忠. 华细辛（异）丁香酚-O-甲基转
移酶（IEMT）基因的分离与功能分析〔J〕. 中国中药杂
志，2024，49（10）：2680-2688.
〔12〕洪鹏. 贮藏条件对柚皮精油影响及主要成分转化分
析〔D〕. 厦门：集美大学，2016.
〔13〕涂雅蕙，贾林金，张君怡，等. 对伞花烃的来源、合成及
生物活性研究与应用进展〔J〕. 食品安全质量检测学
报，2024，15（14）：203-212.
〔14〕鄢洪德. 烯还原酶不对称还原（R）-香芹酮的研究〔J〕.
发酵科技通讯，2024，53（2）：74-78.
〔15〕 ZÁMBORINÉ NÉMETH É，THI NGUYEN H. Thujone，a
Widely Debated Volatile Compound：What Do We Know
about It?〔J〕. Phytochem Rev，2020，19（2）：405-423.
〔16〕刘兴勇，陈兴连，杜丽娟，等. 多因素交互作用导致烘焙
咖啡中绿原酸快速降解〔J〕. 食品科学，2021，42（9）：
7-14.

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