Formulation Optimization and Wound Healing Effect of a Periplaneta americana Composite#br#
Spray Film in Rats

doi:10. 3969 / j. issn. 2096-2266. 2025. 08. 002

Abstract

Abstract: Objective: To prepare a Periplaneta americana composite spray film, optimize its formulation, and assess its efficacy in
wound healing of rats. Methods: Single-factor experiments were conducted to screen film-forming excipients based on evaluation
indices including spray atomization performance, film-forming time, moisturizing capacity, and mechanical properties of the film. The
formulation ratios were further optimized by response surface methodology. A rat skin wound model was established to evaluate the
wound healing effect of the optimized spray film. Results: The optimal film-forming excipients were determined to be Bletilla striata
polysaccharide （BSP）, polyvinylpyrrolidone K30 （PVPK30）, and poloxamer 188 （P188）, with glycerol as the plasticizer and benzoic
acid as the preservative. The optimized formulation comprised 5.0% PVPK30, 2.0% P188, 0.7% BSP, 3.0% P. americana extract, 4.0%
glycerol, and 0.1% benzoic acid. The wound healing study showed that the spray film significantly promoted wound closure and healing
in rats. Conclusion: The optimized P. americana composite spray film demonstrated stable preparation technology, excellent filmforming
ability, and significant promoting effects on acute skin wound healing in rats.

Key words: Periplaneta americana, spray film, wound healing, Bletilla striata polysaccharide, response surface methodology

CLC Number:

R285

Zhang Jingyu, , Chen Zhongze, Li Kailing, He Rongmao, Zhao Zhitao, Yang Yongshou, Xiao Peiyun. Formulation Optimization and Wound Healing Effect of a Periplaneta americana Composite#br# Spray Film in Rats[J]. Journal of Dali University, 2025, 10(8): 11-19.

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Formulation Optimization and Wound Healing Effect of a Periplaneta americana Composite#br# Spray Film in Rats

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