Influence Factor Study of the Determination of Free Amino Acids in Periplaneta Americana Medicine

Abstract

Abstract:

Objective: To study the influence factors of the determination of free amino acids in Periplaneta americana medicine and find out the best testing condition. Methods: Single factor experiment and orthogonal test design were applied to determine the solvent concentration, material ratio, extraction time, extraction times, active carbon dosage, decoloring time, pH value of the buffer solvent and coloring time. Results: The optimum extraction condition for free amino acids from the Periplaneta americana is that reflux extraction is applied to the 8 times of 65% ethanol for 3 times with each time lasting 60 minutes; the optimum testing condition lies when the ratio between active carbon and the sample is 4:100 in the 10 minutes' decolorization, and the pH value of the butter
solvent is 6.0 in coloring and then water bath heating is applied for 30 minutes. Conclusion: The extraction and testing conditions are suitable for testing the amount of free amino acids in Periplaneta americana.

Key words: Periplaneta americana, free amino acids, content determination, influence factor

CLC Number:

R284.2

WANG Jia-Dang, LI Yan-Ling, HE Yong-Mei, SUN Xiao-Mei, YANG Yong-Shou. Influence Factor Study of the Determination of Free Amino Acids in Periplaneta Americana Medicine[J]. J4, 2011, 10(12): 12-15.

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