Abstract:

Objective: To establish a method for the measurement of oleanolic acid and ursolic acid in loquat leaf extract by RPHigh Performance liquid chromatography (RP-HPLC). Methods: With the RP-HPLC method, the chromatographic column was applied Agela technologies lnc XBP-venusil C18 (250 mm×4.6 mm, 5 μm); the mobile phase was applied methanol (0.05 mol/L) and ammonium acetate solution (88:12); the flow rate was 0.8 mL/min; and the detected wavelength was 215 nm, the column temperature was 25 °C. Results: The data showed a good linear relationship between peak area and concentration of oleanolic acid within 2.6-26
μg, r=0.999 1; and ursolic acid wintin 0.825-8.24 μg, r=0.999 5. The average recoveries were 99.53% and 99.37%, and RSD were 0.28% and 0.53%, respectively. Conclusion: The RP -HPLC method was simple, rapid, and with strong specificity and good reproducibility which can be applied in content determination for oleanolic acid and ursolic acid of Loquat leaf extract.

Key words: loquat leaf extract, oleanolic acid, ursolic acid, high performance liquid chromatography