J4 ›› 2011, Vol. 10 ›› Issue (12): 30-31.

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Determination of Total Alkaloids Content in Senecio Scandens Buch.-Ham. by Acid Dye Colorimetry

Objective: To determine the total alkaloids content in Senecio scandens Buch.-Ham. Methods: Using the oxymatrine as standard, acid dye colorimetry was used to determine the total alkaloids content in S. scandens at the wavelength of 422 nm. Results:The concentration of oxymatrine showed good linearity in the range of 6.75-135 μg （r=0.999 2）, the average recovery was 100.0%（RSD=1.54%）. Conclusion: The method is simple, reliable and can be used for determination of the total alkaloids in S. scandens.   

  1. School of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China
  • Received:2011-04-28 Revised:2011-09-17 Online:2011-12-15 Published:2011-12-15

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Abstract:

Objective: To determine the total alkaloids content in Senecio scandens Buch.-Ham. Methods: Using the oxymatrine as standard, acid dye colorimetry was used to determine the total alkaloids content in S. scandens at the wavelength of 422 nm. Results:The concentration of oxymatrine showed good linearity in the range of 6.75-135 μg （r=0.999 2）, the average recovery was 100.0%（RSD=1.54%）. Conclusion: The method is simple, reliable and can be used for determination of the total alkaloids in S. scandens.

Key words: acid dye colorimetry, Senecio scandens Buch.-Ham., total alkaloids

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