J4 ›› 2012, Vol. 11 ›› Issue (9): 98-100.
Objective: To establish a HPLC method for content determination of chlorogenic acid in Xiaoerjinqiao Granules.
Methods: We applied the Agilent XBP-C18(4.6 mm×250 mm, 5μm)as the chromatographic column, and the mobile phase with acetonitrile-0.4% phosphoric acid solution(12:88)at the flow rate of 1.0 mL/min under the detection wavelength of 327 nm, and the column temperature of 25 0C. Results: There was a good linear relationship in the concentration range of 0.05~5.00 μg/mL (r =1.0). The average recovery was 100.44% and RSD was 1.50%. Conclusion: The method appears to be simple, accurate and reliable, and can be used for the quality control of Xiaoerjinqiao Granules.