Abstract:

Objective: To establish a method for determining of glycosaminoglycan in active extracts ML-D with anti-hepatic-fibrosis from Periplaneta americana. Methods: Phenol-vitriol colorimetry was used for determining content of glycosaminoglycan; orthogonal design L9（34）was adopted to investigate hydrolysis process of glycosaminoglycan in ML-D; single factor experiment was used to investigate time of color, dosage of phenol and vitriolic. Results: For the best hydrolysis process of glycosaminoglycan in ML-D, the density of hydrochloric acid is 6 mol/L, Solid-liquid ratio is 1:1, and time of hydrolysis is 1 h. There are the best conditions of color, the dosage of phenol and vitriolic respectively is 2 mL and 20 mL, heating time is 10 min. Conclusion: The method is simple and accurate with reproducibility; it can be used for the determining content of glycosaminoglycan in active extracts ML-D with anti-hepatic-fibrosis from
Periplaneta americana.

Key words: Periplaneta americana, anti-hepatic-fibrosis, glycosaminoglycan, phenol-vitriol colorimetry, content determination