Construction of the Recombinant Bb-ipaB Vaccine of Shigella flexneri

Abstract

Abstract:

Objective：To construct the recombinant Bb-ipaB vaccine of Shigella flexneri. Methods：ipaB gene was amplified by PCR and cloned into pGEX-1λT plasmid to construct pGEX-ipaB. The recombinant plasmid was electroporated into Bifidobacteria bifidum（Bb）to construct rBb-ipaB vaccine. Results：A 1 711 bp gene of ipaB was successfully amplified by PCR and cloned into pGEX-1λT by restriction analysis, and the rBb-ipaB vaccine was successfully constructed by PCR and restriction analysis. Conclusion：The rBbipaB vaccine of Shigella flexneri is successfully constructed, which lays the experimental foundation of exploitation and utilization of this vaccine.

Key words: Shigella flexneri, Bifidobacterium bifidum, recombinant Bb-ipaB vaccine, construction

CLC Number:

R378.2+5

WANG Guo-Fu, XUE Shi-Peng, BAI Li, WU Li-Xian. Construction of the Recombinant Bb-ipaB Vaccine of Shigella flexneri[J]. J4, 2013, 12(3): 13-15.

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