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Table of Content

    15 March 2013, Volume 12 Issue 3
    The Grey Relational Analysis of Element Spectra from Several Isodon Plants
    LIANG Rong, XIE Yong- , JI Shou-Lian, JIANG Bei
    2013, 12(3):  1-2. 
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    Objective: To investigate the relationship of trace elements spectra among the Isodon plants collected from Dali. Methods:Eleven trace elements of the six Isodon samples were determined by using flame atomic absorption spectrophotometer. Based on the content of the trace elements, the correlation of the Isodon samples was evaluated by grey relation analysis. Results: The trace elements spectra of the rhizomes and roots of I. yuennanensis, I. adenantha, and I. loxothyrsa showed close relationship. Conclusion: Results from this study could be useful for the evaluation of physiological mechanism and medicinal values of the underground part of Isodon plants.

    Quantitative Determination of Total Flavonoids in Sangjiang Ganmao Capsule
    ZHU Kun-Ning, CHEN Yuan-Yuan, ZHOU Yang, YANG Yong-Shou
    2013, 12(3):  3-5. 
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    Objective: To establish UV spectrophotometry method for the determination total flavonoids in compounds of Sangjiang Ganmao capsule. Methods: The contents of the total flavonoids were determined at 506 nm with rutin as the standard substance by ultraviolet UV-visible method. Results: The calibration curve was linear in the range of 0.5-3.0 mg for rutin, r=0.999 7; the average recovery was 96.63%, RSD=1.91%(n=6). Conclusion: This method is simple, fast and accurate, and can be used as the determination of the total flavonoids in compound Sangjiang Ganmao capsule.

    Vitro Antibacterial Research of Roots, Stems, and Leaves of Aqueous Extract of Paederia yunnanensis
    MAO Cai-Yan, YUAN Zhi-Qiong, LIN Li-Jia, SHEN Yuan-Ying
    2013, 12(3):  6-8. 
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    Objective: To study the bacteriostatic and bactericidal effects in vitro of aqueous extract of Paederia yunnanensis. Methods: Solution dilution method is used to measure the bacteriostatic and bactericidal effects of roots, stems and leaves of Paederia yunnanensis against Staphylococcus aureus, Shigella dysenteriae, Escherichia coli, and Pseudomonas aeruginosa. Results: All of three parts have bacteriostatic and bactericidal effects on the four experimental bacteria, but there are also significant differences. The root of Paederia yunnanensis has the strongest bacteriostatic and bactericidal action against Pseudomonas aeruginosa, and it is also the part with the best bacteriostatic and bactericidal effect for four experimental bacteria as a whole. The leaves of Paederia yunnanensis have the strongest bacteriostatic action against Shigella dysenteriae, and the stongest bactericidal action against Staphylococcus aureus. Conclusion: The roots, stems, and leaves of Paederia yunnanensis have bacteriostatic and bactericidal actions against four experimental baceria, but also there are differences among their effects, and to provide the basis for the next step of antibacterial medicine standardization.

    Determination of Glycosaminoglycan in Active Extracts ML-D with Anti-hepatic-fibrosis from Periplaneta americana
    LI Kua-Qiao, LI Chun-Yan, YANG Yong-Shou, CHEN Li-Na, XIAO Pei-Yun
    2013, 12(3):  9-12. 
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    Objective: To establish a method for determining of glycosaminoglycan in active extracts ML-D with anti-hepatic-fibrosis from Periplaneta americana. Methods: Phenol-vitriol colorimetry was used for determining content of glycosaminoglycan; orthogonal design L9(34)was adopted to investigate hydrolysis process of glycosaminoglycan in ML-D; single factor experiment was used to investigate time of color, dosage of phenol and vitriolic. Results: For the best hydrolysis process of glycosaminoglycan in ML-D, the density of hydrochloric acid is 6 mol/L, Solid-liquid ratio is 1:1, and time of hydrolysis is 1 h. There are the best conditions of color, the dosage of phenol and vitriolic respectively is 2 mL and 20 mL, heating time is 10 min. Conclusion: The method is simple and accurate with reproducibility; it can be used for the determining content of glycosaminoglycan in active extracts ML-D with anti-hepatic-fibrosis from
    Periplaneta americana.

    Construction of the Recombinant Bb-ipaB Vaccine of Shigella flexneri
    WANG Guo-Fu, XUE Shi-Peng, BAI Li, WU Li-Xian
    2013, 12(3):  13-15. 
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    Objective:To construct the recombinant Bb-ipaB vaccine of Shigella flexneri. Methods:ipaB gene was amplified by PCR and cloned into pGEX-1λT plasmid to construct pGEX-ipaB. The recombinant plasmid was electroporated into Bifidobacteria bifidum(Bb)to construct rBb-ipaB vaccine. Results:A 1 711 bp gene of ipaB was successfully amplified by PCR and cloned into pGEX-1λT by restriction analysis, and the rBb-ipaB vaccine was successfully constructed by PCR and restriction analysis. Conclusion:The rBbipaB vaccine of Shigella flexneri is successfully constructed, which lays the experimental foundation of exploitation and utilization of this vaccine.

    Expression and Significance of Histone Deacetylase 1 , 8 in Alveolar Epithelial Type II Cells Mesenchymal Transition
    JIANG Wei, XU Guo-Ping
    2013, 12(3):  16-19. 
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    Objective: To observe the expression of histone deacetylase 1,8 on the process of TGF-β1 induced type II alveolar
    epithelial cells to EMT, and investigate the effect of TSA on TGF-β1-induced the cells EMT process. Methods: RLE-6TN cell lines were cultured and treated with TGF-β1, and collected cells at different time points. The expression of markers and mRNA of E-cad,α-SMA,HDAC1 and HDAC8 were assayed by Western Blot and Real-time RT-PCR, respectively. Then add TGF-β1 to the cells after TSA pretreatment for 6 hours and repeat the above assay. Results: With the treatment of TGF-β1, the expression of E-cad protein was down regulated; α-SMA, HDAC1 and HDAC8 protein was unregulated. E-cad mRNA was down regulated; both the mRNA of α-SMA and HDAC8 were up regulated at 12 h, and were down regulated at 6 h, 24 h; HDAC1 mRNA at 6 h were down regulated, 24 h was up regulated. After pretreated with TSA, the expression of E-cad protein was up regulated; α-SMA, HDAC1 and HDAC8 protein was down
    regulated. E-cad mRNA was up regulated; α-SMA mRNA at 6 h, 12 h was up regulated, 24 h was down regulated; HDAC1 mRNA was down regulated; HDAC8 mRNA was up regulated. Conclusion: It is possible to use TSA inhibited RLE-6TN cells to receive α-SMA phenotype, loss of E-cad phenotype, which partially reverse the EMT of RLE-6TN induced by TGF-β1 in vitro.

    Distribution and Host Selection of Leptotrombidium yui in Some Areas of Yunnan Province
    GUO Bin, GENG Ming-Lu, GUO Xian-Guo
    2013, 12(3):  20-25. 
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    Objective: To investigate the geographical distribution and host selection of Leptotrombidium yui among different small mammal hosts in some areas of Yunnan province. Methods: A field survey was carried out in 23 counties of Yunnan province between 2001 and 2011. The small mammal hosts were captured with mouse cages and traps with baits. The chigger mites on the surface of two auricles were scraped off by a bistoury, and then preserved in 70%ethanol.Every specimen of the chigger mites on the slides was finally identified into species under a microscope. Some conventional statistical methods were adopted to calculate all the collected chigger mite species and the constituent ratios of L. yui in different areas and on different hosts, together with its prevalence(P), mean intensity(MI)and mean abundance (MA)on different hosts. The patch index (m*/m)was used to measure the spatial patterns of L. yui among the different individuals of its small mammal hosts. Results: A total of 108 480 individuals of chigger mites were collected from the body surface of all the captured small mammal hosts. All the collected chigger mites were identified as 3 subfamilies, 24 genera and 234 species. Of 234 species of chigger mites, 1 959 individuals of L. yui were collected only in 9 counties. The collected individuals of L. yui accounted for 1.81% of the total mites (108 480 individuals). 88.77%of L. yui came from the mountainous regions and habitats while only 11.23% of L. yui from flatland areas. The hosts in orders Rodentia and Scandentia harbored 57.78% and 42.16% of the collected L. yui. Of 67 species(in 34 genera and 12 families of 5 orders), Tupaia belangeri(in Order Scandentia)harbored 42.16% of the collected L. yui with relatively high prevalence (P=26.52%)and mean intensity (MI=13.54 mites / per host), and other wild-dwelling small mammals came next. The patch index showed that L. yui had an aggregated distribution pattern among different individual of their hosts. Conclusion: L. yui exists in Yunnan province with relatively a considerable number of individuals. In Yunnan province, L. yui is mainly distributed in the
    mountainous regions and habitats with relatively high altitude. L. yui can parasitize several different species of hosts with low host specificity, but it prefers to choose Tupaia belangeri and other wild-dwelling small mammals as its main hosts.

    Causes for Frequent Students' Athletic Sudden Death and Its Preventive Measures
    DONG Yu-Fu
    2013, 12(3):  26-30. 
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    Objective: To explore causes for frequent students' athletic sudden death and its preventive measures in recent years.
    Methods: Literature review was adopted to analyze the reasons and preventive measures. Results: The causes for frequent students' athletic sudden death are in the following aspects: the improvement of the living conditions and medical conditions makes the students with potential disease problems increasing, who become a high-risk group of exercise-related sudden death; the lack of exercise and physical weakness make students' ability to withstand the sports load getting worse; ignoring the preparatory activities and organize activities make sports process lack integrity; the opportunities to participate in various large-scale events are increasing, excess load movement is easy to cause myocardial injury; irregular life style. Conclusion: The preventive measures are in the following aspects: strengthen publicity and education of sports health care knowledge, improve students' ability of self-monitoring and on-the-spot first aid; improve the students physical examination content, prohibit students at high risk of disease to participate in strenuous exercise and competition; pay attention to prevention and investigation of exercise-induced cardiac damage; strengthen the school sports education, improve the students ability to bear the load; pay attention to identification of the prodromal symptoms of exercise-related sudden death; promote a healthy lifestyle.

    An Investigation of Causes of Knee Joint Injuries of Male Athletes in College Basketball Teams of Yunnan Province
    and the Study of Countermeasures
    HONG Xi-Jun, WANG Shi-Chao
    2013, 12(3):  31-34. 
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    Objective: To provide advices for the training of college basketball team of Yunnan province, reducing the injury of athletics and prolonging the sport life. Methods: Document research, field research, questionnaire. Results: The factors which cause male athletics of Yunnan basketball teams injuring are unreasonable warming-up, carrying more weight beyond their ability, insisting training and gaming with injury, body play, inadequacy skills, poor physical condition, body fatigue, inattention, poor awareness of self-protecting, poor self-medical inspection, incomplete space facilities, oligotrophy and lower quality of sports equipment. Conclusion: We should raise awareness with strictly study, have reasonable training, emphasize skills practicing, provide enough nutrition and rest, and improve the
    sports facilities.

    Survey and Analysis on the Sanitary Conditions about Children's Food in Dali Country
    YUAN Zhi-Qiong, LI Yun-Cheng, SHEN Yuan-Ying
    2013, 12(3):  35-38. 
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    Objective: To learn about children's food safety in Dali rural area so as to provide the reference for preventive measures of improving it in rural areas. Methods: The stratified sampling was combined with random sampling to survey children's food with questionnaire in six counties (five towns in each). The total of questionnaire was 2 200, and recovered all; the recovery rate was 100%; effective questionnaire was 2 179; the effective rate was 99%. Results: There were 33 rural canteens within hygiene license from 52 canteens in 26 countries(64%)(χ2=1067.642, P=0.000<0.01). The education level of food operators was generally low. Only 19.5 percent of them were with high school and college educational background; 46.4% with junior high school educational background; illiterate persons was 9.2%(χ2=294.332, P=0.000<0.01).There were serious problems of children's food packaging from the 2 179 sample survey of rural primary schools, such as lack of the date of manufacture and inspection certificate, stained or damaged packaging. There was significant difference between different counties (P<0.01). Conclusion: There are serious problems existing in children's food safety in Dali rural areas. So the relevant departments should strengthen supervision and control; parents and school should strengthen the education about food safety for the pupils.

    Significance and Examination of Carotid of Vascular Dementia with Color Doppler Ultrasound
    ZHAO Hong-Nian, LI Dan-Dan, ZHANG Xiao-Yan, ZHONG Jian
    2013, 12(3):  39-41. 
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    Objective: To investigate the correlation of vascular factors and vascular dementia by the carotid artery color Doppler.
    Methods: The carotid arteries in 34 patients with VD and 20 normal controls (NC group)were examined by the carotid artery color Doppler. Results: Peak systolic velocity(PSV)and drag index(RI)of bilateral common carotid artery and bilateral internal carotid artery in VaD group were greater than those in the normal group. PSV and RI of left common carotid artery and RI of left internal carotid artery in VaD group were greater than those in the normal group (P<0.05). Intima media thickness (IMT)of bilateral carotid artery in VD group was higher than those in the normal group(P<0.05). Conclusion: Blood circulation of the carotid artery may be related to the pathogenesis of VD.

    Percutaneous Cystolithotomy for Pediatric Bladder Stone
    YANG Li, XIAO Zhi-Song, ZHENG Bao-Shou, WEN Gui-Cheng
    2013, 12(3):  42-44. 
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    Objective: To evaluate the application of percutaneous cystolithotomy in pediatric bladder stone. Methods: Fifteen boys with a mean age of 4-9(6.6±1.57)years and mean stone diameter at 1.3-3.7(2.6±0.92)cm underwent percutaneous cystolithotomy. Patients were punctureed into the bladder above suprapubic for establish the percutaneous bladder channel, then the bladder stones were smashed with the methods of holmium laser lithotripsy or pneumatic ballistic lithotripsy. The indwelling catheter was implanted according to intraoperative situation. Total 15 cases of clinical data were conducted with retrospective analysis. Results: All 15 patients were operated successfully and the mean procedure time was 20~45(32.5±6.43)min. Five cases felt pain because of indwelling catheter but there were no severe complications occurred. During 6~12 months follow-up, there were no difficulties in urinating, no urethral stricture, and no stone recurrence. Conclusion: Percutaneous cystolithotomy is an effective therapy in the treatment of pediatric bladder stones, which is with less trauma, high stone removal rate. The therapy can avoid the risks induced by transurethral technique such as urethral mucosal damage, postoperative residual stones and urethral stricture.

    An Analysis of Maternal Follow-up Results of 4760 Cases of Prenatal Screening and Diagnosis
    ZHANG Fang-Fang, TU Yi-Fei, HOU Li-Na, XIONG De-Qing, XU Yong-Qin, FANG Qian
    2013, 12(3):  45-47. 
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    Objective: To explore clinical application and significance of prenatal screening and diagnosis. Methods: Time-resolved method was used with serum AFP, β- HCG as indicators in 4 760 pregnant women with pregnancy15-20+6weeks for prenatal screening. For high risk pregnant women, genetic counseling and prenatal diagnosis were provided, and in the postpartum period, the delivery outcome follow-up observation was done. Results: In the follow-up, we found 29 cases of varying degrees of deformity, abortion, stillbirth and other adverse pregnancy outcomes. 6 cases were of high risk; 23 were of low risk. The amniotic fluid chromosome abnormalities existed in 2 cases; ultrasonic examination in detection of various malformations: open neural tube defects such as spina bifida, microcephaly, encephaloc in 6 cases; visceral malformations such as congenital heart disease, renal dysplasia, intrauterine fetal growth abnormalities in 5 cases; an unexplained stillbirth, late abortion in 3 cases. 21- trisomy 1 newborn in 1 case (prenatal screening for Down's risk to the amniotic fluid puncture cytology). Conclusion: Prenatal screening and prenatal diagnosis can decrease the incidence of birth defects.

    36 Cases Diagnosis of Para Pharyngeal Space Tumors
    MING Cheng, RUAN Biao, DAI Xue-Zhong, BAO Xue-Yu, ZHOU Yang, DENG Fang
    2013, 12(3):  48-50. 
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    Objective: To learn about anatomic features of the parapharyngeal space and to explore the diagnosis of parapharyngeal space tumors. Methods: Retrospective review was done on the clinical pictures, radiological features, surgical approaches, surgical findings, and postoperative complications of 36 parapharyngeal space tumors. All patients were followed up from 6 months to 3 years. Results: Benign tumors included 31 cases; malignant tumors included 5 cases. Conclusion: According to the location, transcervical surgical approach may be used for treating parapharyngeal space tumors.

    Nasal Endoscopic Operation in the Treatment of Nasal Mucosa Contact Point Headache
    CHEN Yin-Zhong, DONG Qin, YU De-Shun, GUO Qing-Hua
    2013, 12(3):  51-53. 
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    Objective: To verify the correlation between nasal mucosa contact point and headache and to discuss the significance and feasibility of nasal endoscopic surgery through observation of clinical outcomes in patients with nasal mycosa contact point treated by endoscopic surgery. Methods: 52 cases of endoscopic operation in treatment of nasal mucosa contact point headache in patients with preoperative and postoperative headache were analyzed. Results: The total efficiency of nasal endoscopic operation in the treatment of nasal mucosa contact point headache was 96.2%for 3-6 months and 82.7%for 12-24 months. Conclusion: Nasal mucosal contact is one of the causes of refractory headache. Nasal endoscopic operation for correction of abnormal anatomic structure can relieve the headache, but nasal mucosal contact is not the only etiology of refractory headache. After operation, patients should require continued follow - up to observe the long - term effect.

    Clinical Observation of Orsalin Ointment Combined with Narrow-band UVB in the Treatment of Plaque Psoriasis
    HUANG Jing, HUANG Ling, WANG Min-Hua, NI Chang-Wen
    2013, 12(3):  54-55. 
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    Objective: To observe the clinical efficacy of Orsalin Ointment combined with narrow-band UVB in the treatment of Plaque Psoriasis. Methods: Their bilateral clinical central study was carried out. 51 patients with plaque psoriasis were randomly divided into two groups and treated for 4 weeks. The test group was treated with orsalin ointment; the control group did not use it. Both of the groups were treated by narrow-band UVB. Results: The efficacy rates were 86. 27% in the treatment group and 50.98% in the test group; there was a significant difference of curative effect and Lesional PASI score in the two groups(P﹤0.05). Conclusion: Orsalin Ointment combined with narrow-band UVB in the treatment of Plaque Psoriasis is highly effective, fast action and safe.

    Analysis on Clinical Manifestation and Auxiliary Examinations of Viral Encephalitis:Report of 48 Cases
    YANG Shao-Xuan, LI Qiong-Xian
    2013, 12(3):  56-58. 
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    Objective: To explore the clinical symptoms and the auxiliary examination of viral encephalitis, providing clinical evidence for the diagnosis and treatment of viral encephalitis. Methods: A clinical data of forty-eight patients with viral encephalitis in our hospital were retrospectively analyzed.Results: The major clinical manifestations included fever, headache, psychiatric symptoms and vomiting. Auxiliary examination revealed Electroencephalogram(EEG)abnormality rate of 86.7%, cerebrospinal fluid(CSF)abnormality rate of 75% and 40%of the abnormal rate of magnetic resonance imaging (MRI). Conclusion: The data showed that the clinical manifestations of viral encephalitis were special. Auxiliary examination of EEG, also CSF, MRI were with great significance in the diagnosis of viral encephalitis.

    Analysis of Resistance of Escherichia Coli and Klebsiella Pneumoniae in Affiliated Hospital of Dali University
    MO Li-Xiao-Fang, ZHANG Qun-Zhi
    2013, 12(3):  59-62. 
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    Objective: To instruct the clinical medicine in Affiliated Hospital of Dali University by analyzing the resistance of Escherichia coli and Klebsiella pneumoniae from 2011 to 2012. Methods: Isolate and culture strains by using conventional methods, and identify the bacteria with VITET-2 Compact 60 automatic microbial analyzer and test the susceptibility through K-B. Results: In total 183 specimens, 146 of them were Escherichia coli: koutan 92(63%), secretions 17(11.6%), urine 16(10.9%), pus 9(6.1% 2), other 12(8.2%); 37 of them were Klebsiella pneumoniae: koutan 27(73%), secretions five(13.5%), pus 3(8%), other susceptibility test results: the resistance rates of Cefoperazone, Amikacin, Imipenem were lower, at 10.3%, 6.2%, 2.7% and 10.8%, 13.5%, 2.7%, respectively. Conclusion: The detection rate of Escherichia coli and Klebsiella pneumoniae in sputum is rising in these two years, and the resistance rates have been exceeded more than 50% of levofloxacin, cefazolin, aztreonam, ceftazidime, especially ceftazidimewas resistance rates is significantly elevated. However, resistance rates of cefoperazone, amikacin, imipenem are lower, so doctors should choose antibiotics based on susceptibility experiments.

    Image Analysis of Aspiration Pneumonia Caused by Oral Clozapine
    LI Yun, SHI Hui-Xian
    2013, 12(3):  63-64. 
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    Objective: To observe the hospitalized psychiatric patients' X-ray image of aspiration pneumonia caused by oral clozapine.Methods: An analysis of 56 hospitalized patients' chest X-ray image from 2010 to 2011 who suffered from aspiration pneumonia caused by oral clozapine was made. Results: Lung lesions in image for single or multiple patchy fuzzy shadow, and salivate is clozapine early treatment of one of the most common adverse reactions, about 64.3% of the patients to salivate, is an important factor caused by aspiration pneumonia. Conclusion: We should closely observe taking clozapine a patient's condition changes, necessary chest X-ray.

    Clinical Application of Single - Person Operation Colonoscopy
    DU Gui-Ying, TAO Zhong-Yuan
    2013, 12(3):  65-66. 
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    Objective: To explore the method and clinical application of single-person operation colonoscopy. Methods:5 752 cases were examined by single-person operation. Results: Among these 5 752 cases, there were 5 435 whose examination arrived at ileocecus. The success rate was 94.48%. 763 cases were with focal symptom; 1 case with intestinal perforation. Conclusion: Single-person operation colonoscopy is a method with advantages of safety, saving time and labor, less-pain, and high rate of success, which is worth popularizing among grass-roots hospitals.

    Practice and Experience of Improving the Comprehensive Quality of Nurses Working in the High-Quality-Nursing Sickroom
    SHEN Ji-Li, SUN Ju-Yan, GAO Mei, JIA Hui-Fang, CAO Lin
    2013, 12(3):  69-72. 
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    Objective: To investigate the ways of improving the comprehensive quality of nurses working in the high-quality care ward. Methods: We chose nurses and patients in two different care modes from Sept. 2010 to Feb. 2011 and Mar. 2011 to Aug. 2011 as the research object. Our own design high-quality-nursing evaluation form and satisfaction questionnaire for discharged patients were applied to collect information. And the professional competence of nurses was comparatively analyzed. Results: There was no significant difference in nursing quality and patients' satisfaction to nurses between new and old care mode (P= 0.478, 0.979). The patients' satisfaction of new care mode was higher than that of old mode(P= 0.022). Nursing operation and theoretical examination results of new nursing mode was all higher than that of old mode(P= 0.042, <0.001). Conclusion: The new care mode, in which the task and responsibility were assigned to individual and group, helps to improve the comprehensive quality of the nurses, to enhance connotation of high-quality-nursing, and worth popularizing.

    Study on the Structure System of Nursing Soft Skills
    FU Yan-Fen, ZHENG Xian-Lan, LI Ping, MENG Yu-Qian- , JIANG Xiao-Ping
    2013, 12(3):  73-75. 
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    Objective: To establish the definition and structure system of nursing soft skills. Methods: The definition and structure
    system of nursing soft skills were defined primarily on the basis of comprehensive analysis of literature and repeated discussion in the research group. Then after two rounds of expert consultation, the definition and structure system of nursing soft skills were revised. Results: The experts average age was 48.65±8.57,average work life 27.00±9.92 and the authority of a factor of 0.823.There were differences in coefficient of concordance(P<0.05).We bring forward definition of the nursing soft skills after twice specialist consultation. An indicator system, 2 first-level indicators,5 second-level indexes and 20 third-level were confirmed based on experts advice. Conclusion: According to research, we bring forward definition of the nursing soft skills and structure system of nursing soft skills.

    On Medical Humanities Education Reform and Innovation
    LV YUE-Jun
    2013, 12(3):  76-79. 
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    In the new century, the integration of the "Medical Ethics Education" and "Humanistic Quality Education" strengthens the medical humanities education, which is the concrete manifestation of promoting the quality education. Meanwhile, we should pay attention to characteristics of medical college, promote medical humanities education and 'continuous line 'of sustainable development, and focus on the medical students' self education, their ability of constantly learning, caring for others, and social service consciousness, which are groundbreaking measures for promoting the quality education of medical students.

    An Analysis of an Effective Teaching Case of University General Education
    YIN Man-Fen
    2013, 12(3):  80-84. 
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    This paper presents a much-welcome and successful course of general education by way of observation and reflection. Based on the feedback from the students, an analysis is conducted concerning the teaching effectiveness which is shown in student-motivated teaching content, student-centered teaching approach and performance-focused formative assessment.

    A Study of the Value of TCSL and Chinese International Promotion for South and Southeast Asia
    ZHAO Ling-Ling
    2013, 12(3):  85-88. 
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    A study of the theoretical and practical value of TCSL and Chinese international promotion for South and Southeast Asia is of great help to pay more attention to the importance and urgency of TCSL and Chinese international promotion, which can provide a solid foundation for constructing an important bridgehead in Yunnan and for carrying out "go global" strategies in Yunnan universities.

    An Investigation on Life Value of Dali University Medical Students
    CHEN Hua-Dong
    2013, 12(3):  89-92. 
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    It is of great importance to implement education of life value for medical students. According to 780 medical students`
    questionnaire from Dali University, we analyzed the status quo of education of life value for medical students. The article has made a summary from the four respects, such as curriculum, social practice, specialty influence and external mechanism, to prove why the education of life value has achieved a better effect.