Expression and Significance of Histone Deacetylase 1 , 8 in Alveolar Epithelial Type II Cells Mesenchymal Transition

Abstract

Abstract:

Objective: To observe the expression of histone deacetylase 1,8 on the process of TGF-β1 induced type II alveolar
epithelial cells to EMT, and investigate the effect of TSA on TGF-β1-induced the cells EMT process. Methods: RLE-6TN cell lines were cultured and treated with TGF-β1, and collected cells at different time points. The expression of markers and mRNA of E-cad，α-SMA，HDAC1 and HDAC8 were assayed by Western Blot and Real-time RT-PCR, respectively. Then add TGF-β1 to the cells after TSA pretreatment for 6 hours and repeat the above assay. Results: With the treatment of TGF-β1, the expression of E-cad protein was down regulated; α-SMA, HDAC1 and HDAC8 protein was unregulated. E-cad mRNA was down regulated; both the mRNA of α-SMA and HDAC8 were up regulated at 12 h, and were down regulated at 6 h, 24 h; HDAC1 mRNA at 6 h were down regulated, 24 h was up regulated. After pretreated with TSA, the expression of E-cad protein was up regulated; α-SMA, HDAC1 and HDAC8 protein was down
regulated. E-cad mRNA was up regulated; α-SMA mRNA at 6 h, 12 h was up regulated, 24 h was down regulated; HDAC1 mRNA was down regulated; HDAC8 mRNA was up regulated. Conclusion: It is possible to use TSA inhibited RLE-6TN cells to receive α-SMA phenotype, loss of E-cad phenotype, which partially reverse the EMT of RLE-6TN induced by TGF-β1 in vitro.

Key words: HDAC1, TGF-β1, TSA, alveolar epithelial type II cells, EMT

CLC Number:

R563.1⁺³

JIANG Wei, XU Guo-Ping. Expression and Significance of Histone Deacetylase 1 , 8 in Alveolar Epithelial Type II Cells Mesenchymal Transition[J]. J4, 2013, 12(3): 16-19.

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