J4 ›› 2013, Vol. 12 ›› Issue (12): 12-15.

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Determination of Content of Total Flavones from the Plant of Arctium lappa L.

  

  1. 1.College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China；
    2.Dali Institute for Food and Drug Control, Dali, Yunnan 671000, China
  • Received:2013-10-23 Online:2013-12-15 Published:2013-12-15

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Abstract:

Objective: To determine the content of the total flavones from the Arctium lappa L.. Methods: The best extraction
technology was determined by orthogonal experiment design, including extraction time，solid-liquid ratio，ethanol concentration and the extraction times. The content of total flavones was determined by absorption spectrometry with rutosids as reference substance.Results: Optimum extraction technology was as follows: 55% ethanol，ratio of material to liquid 1：70，time 1.5 h，two times. The calibration curve had good linear relationship in the range of 4.00~100.00 μg/mL. The average recovery was 97.7%,RSD=2.5%（n=6）.Conclusion: The extraction process is stable and feasible. The quantitative method is accurate.

Key words: Arctium lappa L., total flavones, extraction technology, contents determination

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