Objective: To make a retrospective analysis of the infection pathogenic bacteria distribution and drug resistance in
hospital intensive care unit (ICU)from January 1, 2012 to December 31, 2012. Methods: The identification of bacteria and susceptibility test were performed by using automatic bioanalysis- VITET-2 Compact 60 and Kirby-Bauer methods; the detection rate and the drug sensitivity results of bacterial were analyzed statistically. Results: 799 ICU specimens, 249 strains of hospital infection isolates, the detection rate of 31.16%. The first three bacteria in these samples were: onion burkholderia bacteria topped (60 strains,24.10%), acinetobacter baumannii followed(55 cases, 22.09%), klebsiella pneumoniae was the third(39 strains, 15.66%). Isolated bacteria in sputum specimen source and pharyngeal swab the most common, followed by venous blood and venous catheter. Onion false unit cell, acinetobacter baumannii, pseudomonas aeruginosa, klebsiella pneumoniae and e. coli, staphylococcus aureus and
staphylococcus aureus were with high resistance to many kinds of antimicrobial agents. Conclusion: The detection rate of fermentation bacteria in nosocomial infection pathogens is highest, followed by klebsiella pneumoniae and its resistance is strong; so the treatment of hospital infection caused by pathogens of infectious diseases should be selected according to results of fungi susceptibility test in vitro sensitive antimicrobial agents, in order to reduce resistance.