关键词: font-family:Times New Roman, ">Ghrelinfont-family:Times New Roman, ">；高糖；细胞损伤；氧化应激；焦亡

Abstract: Objective： To observe the protective effect of Ghrelin on high glucose-induced hepatocellular injury and preliminarily explore its mechanism. Methods： A high glucose-induced hepatocellular injury model was established and Ghrelin（10-7 mmol / L） was used for intervention. The experiment were randomly divided into blank control group， Ghrelin + blank control group， high glucose group and Ghrelin + high glucose group. The cell proliferation rate and expression levels of alanine aminotransferase（ALT）， malondialdehyde（MDA） and superoxide dismutase（SOD） were detected. The mRNA expression levels of cysteinyl aspartate specific proteinase（Caspase-1）， interleukin-18（IL-18） and interleukin-1β（IL-1β） were determined by real-time fluorescence quantitative polymerase chain reaction， and the expression of gasdermin D（GSDMD） protein was detected by immunofluorescence technique. Results： Ghrelin could decrease the content of ALT and MDA， increas the SOD content， and significantly reduce the expression levels of pyroptosis-related factors Caspase-1， IL-18， IL-1β mRNA and GSDMD protein. Conclusion： Ghrelin has a protective effect on high glucose-induced hepatocellular injury， and one of its mechanisms may be to relieve oxidative stress and inhibit Caspase-1-mediated pyroptosis.

Key words: font-family:Times New Roman, ">Ghrelinfont-family:Times New Roman, ">；font-family:Times New Roman, "> high glucosefont-family:Times New Roman, ">；font-family:Times New Roman, "> cell injuryfont-family:Times New Roman, ">；font-family:Times New Roman, "> oxidative stressfont-family:Times New Roman, ">；font-family:Times New Roman, "> pyroptosis