Abstract: Objective： To establish a method for the simultaneous determination of seven constituents content in Quban Yangyan capsule. Methods： HPLC method was adopted. The determination was performed on Yi Lite C18 column（4.6 mm×250 mm，5 μm）with mobile phase consisted of 0.1% phosphoric acid solution（A）-acetonitrile （B）（gradient elution） at the flow rate of 1.0 mL/min. The column temperature was set at 25 ℃，and the detection wavelength was set at 232 nm. The sample size was 10 μL. Results： Gallic acid，sodium Danshensu， geniposide， albiflorin，paeoniflorin，ferulic acid and salvianolic acid B showed good linear relationships within the ranges of 3.198-79.950 μg/mL（r=0.999 3）、3.798-94.950 μg/mL（r=0.999 9）、16.638-415.950 μg/mL（r=0.999 9）、6.772-169.300 μg/mL（r=0.999 5）、19.406-485.150 μg/mL（r=0.999 0）、1.612-40.300 μg/mL（r=1.000 0）、10.582-264.550 μg/mL（r=0.999 9），respectively. RSDs of precision，reproducibility， stability and added recovery tests were lower than 2.0％， which were qualified for quantitative testing. The average recoveries were 99.06%（RSD=0.73%），99.89%（RSD=0.54%），98.54%（RSD=0.90%），99.03%（RSD=0.74%），99.79%（RSD=0.56%），100.88%（RSD=0.54%） and 99.28%（RSD=0.48%）. Conclusions： This method is simple， specific， and has high precision， good separation and reproducibility， and can be used to determine the content of seven constituents in Quban Yangyan capsule.

Key words: Quban Yangyan capsule, content determination, HPLC