Therapeutic Effect of Miao Medicine Rubus pluribracteatus on Mice with Ulcerative Colitis

Abstract

Abstract:

Objective： To investigate the therapeutic effect and mechanism of Miao medicine Rubus pluribracteatus on dextran sodium sulfate （DSS）-induced ulcerative colitis （UC） in mice. Methods： Fifty male C57BL/6 mice were randomly divided into control group， model group， mesalazine group， R. pluribracteatus low-dose group and R. pluribracteatus high-dose group， with 10 mice in each group. The control group drank water for 7 days， and the other groups were given 3%DSS solution for 7 days to construct UC mouse model. Thereafter， each group was gavaged for 7 days. During the modeling and administration period， the disease activity index（DAI） of mice in each group was measured. After the last administration， blood extraction of inner canthus was completed and the mice in each group were sacrificed and the whole colon（from cecum to anus） was cut to measure the length of the colon. Hematoxylin and eosin staining was used to observe the pathological morphology， enzyme linked immunosorbent assay was used to detect the levels of tumor necrosis factor-α（TNF-α） and diamine oxidase（DAO） in serum， and Western blot was used to detect the expression of Occludin， mucin 2（Muc2）， and zonula occludens-1（ZO-1） in colon tissue. Results： R. pluribracteatus could reduce DAI scores of UC mice， increase the length of the colon，alleviate the histological changes of colon tissue， decrease the levels of TNF-α and DAO in serum， and upregulate the expression levels of Occludin， Muc2 and ZO-1. Conclusion： Miao medicine R. pluribracteatus may treat UC by promoting the expression of Occludin， Muc2 and ZO-1 proteins and improving intestinal mucosal barrier function.

Key words:

"> font-size:10.5pt, ">ulcerative colitis, Rubus pluribracteatus, Occludin, mucin 2 , zonula occludens-1font-size:10.5pt, ">

CLC Number:

Xiong Kai, Xiao Tianbao, Zeng Lian, Luo Zicheng, Yang Tao, Yang Shengbang.

Therapeutic Effect of Miao Medicine Rubus pluribracteatus on Mice with Ulcerative Colitis [J]. Journal of Dali University, 2023, 8(4): 1-6.

References

〔1〕张平，方琼，周华. 水溶性蜂胶对酒精加重溃疡性结肠炎发作的影响和机制研究〔J〕. 大理大学学报，2022，7（8）：21-27.

〔2〕 PAONE P， CANI P D. Mucus Barrier， Mucins and Gut Microbiota： The Expected Slimy Partners? 〔J〕. Gut， 2020，

69（12）：2232-2243.

〔3〕 TAN Y， GUAN Y， SUN Y， et al. Correlation of Intestinal Mucosal Healing and Tight Junction Protein Expression in Ulcerative Colitis Patients〔J〕. Am J Med Sci， 2019，357（3）：195-204.

〔4〕 YAO D， DAI W， DONG M， et al. MUC2 and Related Bacterial Factors： Therapeutic Targets for Ulcerative Colitis〔J〕. EBioMedicine， 2021，74：103751.

〔5〕 UNGARO R， MEHANDRU S， ALLEN P B， et al. Ulcerative Ｃolitis〔J〕. Lancet，2017，389（10080）：1756-1770.

〔6〕 DU L， HA C. Epidemiology and Pathogenesis of Ulcerative Colitis〔J〕. Gastroenterol Clin North Am， 2020，49（4）：643-654.

〔7〕杜士杰，周郭宁，杨来娣，等. 苗药大乌泡茎乙醇提取物的化学成分研究〔J〕. 中国药房， 2018，29（12）：1644-1647.

〔8〕傅立国，陈潭清，郎楷永，等.中国高等植物：第6卷〔M〕.

青岛：青岛出版社， 2003.

〔9〕 YANG T， MA X， WANG R， et al. Berberine Inhibits IFN-γ Signaling Pathway in DSS-Induced Ulcerative Colitis〔J〕. Saudi Pharm J， 2022，30（6）：764-778.

〔10〕 S?魣NCHEZ DE MEDINA F， ROMERO-CALVO I， MASCARAQUE C， et al. Intestinal Inflammation and Mucosal Barrier Function〔J〕. Inflamm Bowel Dis， 2014，

20（12）：2394-2404.

〔11〕 VANCAMELBEKE M， VANUYTSEL T， FARR?魪R， et al. Genetic and Transcriptomic Bases of Intestinal Epithelial Barrier Dysfunction in Inflammatory Bowel Disease〔J〕. Inflamm Bowel Dis， 2017，23（10）：1718-1729.

〔12〕 NYSTR?魻M E， MARTINEZ-ABAD B， ARIKE L， et al. An Intercrypt Subpopulation of Goblet Cells Is Essential for Colonic Mucus Barrier Function〔J〕. Science， 2021，

372（6539）：eabb1590.

〔13〕 VAN DER SLUIS M， DE KONING B A， DE BRUIJN A C， et al. Muc2-Deficient Mice Spontaneously Develop Colitis， Indicating That MUC2 Is Critical for Colonic Protection〔J〕. Gastroenterology， 2006，131（1）：117-129.

〔14〕 LEON-CORIA A， KUMAR M， WORKENTINE M， et al. Muc2 Mucin and Nonmucin Microbiota Confer Distinct Innate Host Defense in Disease Susceptibility and Colonic Injury〔J〕. Cell Mol Gastroenterol Hepatol， 2021，11（1）：77-98.

〔15〕 VAN KLINKEN B J， VAN DER WAL J W， EINERHAND A W， et al. Sulphation and Secretion of the Predominant Secretory Human Colonic Mucin MUC2 in Ulcerative Colitis〔J〕. Gut， 1999，44（3）：387-393.

〔16〕 KAMINSKY L W， AL-SADI R， MA T Y. IL-1β and the Intestinal Epithelial Tight Junction Barrier〔J〕. Front Immunol， 2021，12：767456.

〔17〕 VETRANO S， CORREALE C， BORRONI E M， et al. Colifagina， a Novel Preparation of 8 Lysed Bacteria Ameliorates Experimental Colitis〔J〕. Int J Immunopathol Pharmacol， 2008，21（2）：401-407.

〔18〕 CHEN H Q， YANG J， ZHANG M， et al. Lactobacillus plantarum Ameliorates Colonic Epithelial Barrier Dysfunction by Modulating the Apical Junctional Complex and PepT1 in IL-10 Knockout Mice〔J〕. Am J Physiol Gastrointest Liver Physiol， 2010，299（6）：G1287-G1297.

〔19〕 GAO M， JIANG Y， XIAO X， et al. Protective Effect of Pioglitazone on Sepsis-Induced Intestinal Injury in a Rodent Model〔J〕. J Surg Res， 2015，195（2）：550-558.

〔20〕 CIARLET M， ESTES M K. Interactions between Rotavirus and Gastrointestinal Cells〔J〕. Curr Opin Micro-biol，2001，4（4）：435-441.

〔21〕 NAKASE H， SATO N， MIZUNO N， et al. The Influence of Cytokines on the Complex Pathology of Ulcerative Colitis〔J〕. Autoimmun Rev， 2022，21（3）：103017.

〔22〕 NEURATH M F. Cytokines in Inflammatory Bowel Disease〔J〕. Nat Rev Immunol， 2014，14（5）：329-342.

[1]	Li Jing, Ling Haowei, Shi Kaixuan, Liu Rong, Xiao Chaojiang, Jiang Bei, . Study on Chemical Constituents of the Root of Agapetes lacei var. tomentella [J]. Journal of Dali University, 2023, 8(8): 1-5.
[2]	Ling Haowei, Li Jing, Shi Kaixuan, Liu Rong, Xiao Chaojiang, Jiang Bei, . Study on Chemical Constituents of Gentiana nanobella [J]. Journal of Dali University, 2023, 8(8): 6-10.
[3]	Shi Kaixuan, Li Jing, Ling Haowei, Liu Rong, Xiao Chaojiang, Jiang Bei, . Study on Chemical Constituents of Leptodermis pilosa [J]. Journal of Dali University, 2023, 8(8): 11-14.
[4]	Qin Zikang, Guo Haihui, Shan Fang, Wu Lei, Wang Fusheng. Synthesis of L-5-Hydroxytryptophan Derivatives [J]. Journal of Dali University, 2023, 8(8): 15-20.
[5]	Shan Fang, Guo Haihui, Qin Zikang, Li Chunxu, Wang Fusheng. Synthesis of Gentiournoside D Amide Derivatives [J]. Journal of Dali University, 2023, 8(8): 21-24.
[6]	Lai Yuxia, Guo Haihui, Qin Zikang, Shan Fang, Wang Fusheng. Optimization of Ｓelective Ester Hydrolysis Conditions for Depresteroside [J]. Journal of Dali University, 2023, 8(8): 25-29.
[7]	Lu Xixing, Zhu Ling, Wu Hao, Zhang Chenggui, Yang Yongshou, Chen Zhipeng, Liu Heng, . Chemical Composition Analysis and Multicomponent Content Determination of Extracts of Periplaneta americana from Different Breeding Plants [J]. Journal of Dali University, 2023, 8(8): 30-38.
[8]	Su Baoshun, Liu Zhitong. Preliminary Study on Antibacterial Effect and Active Parts of Dobinea delavayi [J]. Journal of Dali University, 2023, 8(8): 39-43.
[9]	Li Xiaoji, Li Guorong. Construction and Effectiveness of the Pharmaceutical Control System of Injection Proton Pump Inhibitors [J]. Journal of Dali University, 2023, 8(8): 44-48.
[10]	Ye Shengying, Wang Xinrui, Gao Jingwen, Qin Yan. The Protective Effect of Ghrelin on High Glucose-Induced Ｈepatocellular Injury [J]. Journal of Dali University, 2023, 8(8): 49-53.
[11]	Deng Wenbin, Huang Shiqiong, Wang Xue, Li Lihua. Effects of High Salt Intervention on Proliferation and Migration of Human Aortic Vascular Smooth Muscle Cell [J]. Journal of Dali University, 2023, 8(8): 54-58.
[12]	Zhi Hongfang, Chen Xin, Zhang Jianyan, Wang Chengcheng, Zhang Yun, Yang Xuantao. Expression of RIP140 and LCOR in High-Grade Cervical Intraepithelial Neoplasia [J]. Journal of Dali University, 2023, 8(8): 59-63.
[13]	Wu Maofang, Shi Wen, Xiao Shouyong, Cai Jingjing, Liu Zengping, Fang Hong. Propensity Score Matching Analysis of Postoperative Radiotherapy in Patients with Stage ⅢA/N2 Non-Small Cell Lung Carcinoma [J]. Journal of Dali University, 2023, 8(8): 64-70.
[14]	Xiao Min, Xiong Miaomiao, Cheng Mingjing, Cui Xiuyu, Zhao Yuanqi, Zhao Weidong. Scientometric Analysis of Ferroptosis Literature Based on Web of Science #br# [J]. Journal of Dali University, 2023, 8(8): 71-77.
[15]	Rao Yanhua, Wu Xingjun, Quan Yuan, Guan Qingni, Wang Suorong, Tian Ying. Study on the Elderly Care Career-Selection Motivation and Influencing Factors of Vocational Nursing Students [J]. Journal of Dali University, 2023, 8(8): 78-83.