Table of Content

15 April 2023, Volume 8 Issue 4

Previous Issue    Next Issue

Therapeutic Effect of Miao Medicine Rubus pluribracteatus on Mice with Ulcerative Colitis

Xiong Kai, Xiao Tianbao, Zeng Lian, Luo Zicheng, Yang Tao, Yang Shengbang

2023, 8(4):  1-6. 

Asbtract ( 175 )   PDF (7546KB) ( 115 )  

References | Related Articles | Metrics

Objective： To investigate the therapeutic effect and mechanism of Miao medicine Rubus pluribracteatus on dextran sodium sulfate （DSS）-induced ulcerative colitis （UC） in mice. Methods： Fifty male C57BL/6 mice were randomly divided into control group， model group， mesalazine group， R. pluribracteatus low-dose group and R. pluribracteatus high-dose group， with 10 mice in each group. The control group drank water for 7 days， and the other groups were given 3%DSS solution for 7 days to construct UC mouse model. Thereafter， each group was gavaged for 7 days. During the modeling and administration period， the disease activity index（DAI） of mice in each group was measured. After the last administration， blood extraction of inner canthus was completed and the mice in each group were sacrificed and the whole colon（from cecum to anus） was cut to measure the length of the colon. Hematoxylin and eosin staining was used to observe the pathological morphology， enzyme linked immunosorbent assay was used to detect the levels of tumor necrosis factor-α（TNF-α） and diamine oxidase（DAO） in serum， and Western blot was used to detect the expression of Occludin， mucin 2（Muc2）， and zonula occludens-1（ZO-1） in colon tissue. Results： R. pluribracteatus could reduce DAI scores of UC mice， increase the length of the colon，alleviate the histological changes of colon tissue， decrease the levels of TNF-α and DAO in serum， and upregulate the expression levels of Occludin， Muc2  and ZO-1. Conclusion： Miao medicine R. pluribracteatus may treat UC by promoting the expression of Occludin， Muc2 and ZO-1 proteins and improving intestinal mucosal barrier function.

Content Determination and Methodological Validation of Snail Glycosaminoglycan

Meng Dong, Li Ya, Wang Chongjuan, Tian Yong, Tian Yuncai, Wu Mingyi

2023, 8(4):  7-12. 

Asbtract ( 103 )   PDF (4239KB) ( 98 )  

References | Related Articles | Metrics

Objective： To determine the glycosaminoglycan content in four snail products by 1，9-dimethylmethylene blue spectrophotometry and to verify the detection method. Methods： The 1，9-dimethylmethylene blue spectrophotometry method was used to determine the glycosaminoglycan content in snails， and the specificity， linearity， reproducibility， precision， accuracy， stability and durability of the method were investigated， and the method was used to determine the glycosaminoglycan content in four snail products. Results： The method had good specificity for the determination of glycosaminoglycan content in snails with the maximum absorption wavelength of 520 nm； the contents of glycosaminoglycan in dried white jade snail products， dried glossy large snail products， freeze-dried white jade snail mucus products and freeze-dried glossy large snail mucus products determined by the method at 520 nm were 4.10%， 1.90%， 15.85%， and 9.31%， respectively. Conclusion： After investigation， the method met the relevant requirements for specificity， linearity， repeatability， precision， recovery rate， stability and durability，and it is easy to operate.

Investigation on the Mechanism of Lemai Granules in the Treatment of Vascular Dementia Based on Network Pharmacology and Molecular Docking

Wang Jinyu, Zhou Longlong, Wang Xuechang, Li Xiaodong, Geng Shuqiong, Xia Conglong

2023, 8(4):  13-21. 

Asbtract ( 131 )   PDF (12766KB) ( 45 )  

References | Related Articles | Metrics

Objective： To investigate the mechanism of Lemai Granules in the treatment of vascular dementia（VD） using network pharmacology and molecular docking. Methods： The active ingredients and targets of Lemai Granules were screened from the TCMSP database， and the relevant targets of VD were obtained from GeneCard， OMIM， DrugBank and other databases. The intersection targets were obtained after merging and deduplication. Cytoscape 3.9.0 software was used for visual analysis of the "drug-active ingredient-target" network and protein-protein interaction network to obtain core active ingredients and targets. R 4.2.1 software was used for GO analysis and KEGG pathway enrichment analysis of intersection targets， and AutoDock Vina 1.5.6 software was used for molecular docking. Results： Quercetin， luteolin， and kaempferol may be the core active ingredients for the treatment of VD by Lemai Granules， and core targets included AKT1， PTGS2， and TNF. GO analysis was mainly related to peptide response and cellular responses to chemical stress. KEGG mainly involved lipid and atherosclerosis， PI3K-Akt signaling pathway， and other pathways. The molecular docking results showed that the binding activity between core active ingredients and core targets was strong. Conclusion： Lemai Granules can exert their therapeutic effects on VD through multiple targets and pathways， mainly involving the suppression of inflammatory reaction and the regulation of neuronal apoptosis.

Expression of SOX9 in the Directed Differentiation of Mouse Hepatic Oval Cell into Biliary Epithelial Cells

Huang Shan, Jin Liquan, Yu Yu, Huang Ji, Zhang Junhua, Chen Yiming

2023, 8(4):  22-27. 

Asbtract ( 99 )   PDF (7480KB) ( 74 )  

References | Related Articles | Metrics

Objective： To investigate the expression changes of SOX9 during the directed differentiation of mouse hepatic oval cell （HOC） into biliary epithelial cell（BEC）. Methods：A mouse HOC activation model was established by 2-acetylaminofluorene gavage and 2/3 partial hepatectomy. HOC were extracted by two-step enzyme digestion and Percoll density gradient centrifugation， and then induced to differentiate into BEC by using epidermal growth factor（EGF）， stem cell growth factor（SCF） and leukemia inhibitory factor（LIF） in vitro. Cell morphological changes before and after differentiation were observed under inverted microscope， BEC were identified by flow cytometry， and the expression of SOX9 before and after differentiation was detected by immunofluorescence.Results： HOC were differentiated into purer BEC up to the 8th generation under the joint action of EGF， SCF and LIF. SOX9 was mainly expressed in the cytoplasm of primary mouse HOC， and gradually entered the nucleus during differentiation， with a significant increase in average light density value. Conclusion： SOX9 enters the nucleus and its expression remarkably increases during differentiation of mouse HOCs into BECs， indicating its possible involvement in the regulation of this differentiation process.

Effects of Periplaneta americana Extract CⅡ-3 on the Biofilm Formation of Candida albicans

Zhao Chunjing, Chen Weiguo, Bai li, Wu Youcong

2023, 8(4):  28-33. 

Asbtract ( 116 )   PDF (11368KB) ( 50 )  

References | Related Articles | Metrics

Objective： To investigate the effect of Periplaneta americana extract CⅡ-3（hereinafter referred to as "CⅡ-3"） on the formation of Candida albicans biofilm， so as to lay a foundation for the development of antifungal biofilm drugs. Methods： CⅡ-3 was added to Candida albicans solution and incubated with biofilm. CⅡ-3 high dose group， CⅡ-3 low dose group， positive control group and negative control group were set up respectively. The effect of CⅡ-3 on the formation of Candida albicans biofilm was detected by morphological observation and MTT method. The inhibition rate of biofilm was calculated to evaluate the effect of different concentrations of CⅡ-3 on Candida albicans biofilm at different growth stages. Results： Candida albicans could still form biofilm-like structure under the action of CⅡ-3. The inhibition rates of biofilm in high dose group were 25.1% and 13.9%， which were lower than the inhibition rate of fluconazole biofilm（79.3%）. When incubated with Candida albicans biofilm at different growth stages， the highest inhibition rate of CⅡ-3 on 8 h biofilms was 61.7%， followed by the inhibition rates on 16 h and 24 h biofilms （35.4% and 21.9%）， while fluconazole only had significant inhibitory effect on 4 h biofilms （inhibition rate of 75.5%）， and had no significant inhibitory effect on biofilms at other growth stages. The inhibitory effect of CⅡ-3 on Candida albicans aggregation and mature biofilm was 2-9 times that of fluconazole. Conclusion： The extract CⅡ-3 mainly inhibits the biofilm formation of Candida albicans in the aggregation and maturation stages， and the inhibition effect does not show a significant dose-effect relationship.

Investigation on the Carrier Rate and Antifungal Susceptibility of Yeast Species Isolated from External Urethral Meatus of Healthy College Students

Bai Zongkai, Lü Neng, Liu Runhua, Luo Yuanxi, Wu Qiuyue, Xu Jing, Wu Youcong

2023, 8(4):  34-38. 

Asbtract ( 160 )   PDF (4609KB) ( 77 )  

References | Related Articles | Metrics

Objective： To investigate the yeast carrier rate， species distribution and susceptibility to common antifungal drugs in external urethral meatus of healthy college students. Methods： A total of 261 healthy university students were randomly recruited from a university in Yunnan Province， and the swabs from urinary meatus were collected for isolation， identification， drug sensitivity testing， and statistical analysis. Results： 39 yeast species were isolated，with a carrier rate of 14.9%；  among them， 6 strains were isolated from 130 male samples， with a carrier rate of 4.6%， while 33 strains were isolated from 131 female samples， with a carrier rate of 25.2%. The difference of carrier rate between different gender groups was statiscally significant（χ2=21.73，P=0.005）. Among 39 yeast species， there were 11 strains of Candida albicans（28.2%）， 24 strains of Candida glabrata（61.5%）， 4 strains of other Candida species（10.3%）. The result showed that the geometric means of the yeast to fluconazole， flurocytosine and amphotericin B were 22.03， 0.16 and 4.37 μg/mL， respectively， and the drug resistance rates of isolates were 15.4%， 0.0% and 100.0%， respectively. Conclusion： The prevalence of Candida colonization among healthy college students at urinary meatus is relatively low， with smooth colony Candida glabrata being the predominant species.The colonization rate of Candida among female students is significantly higher than that among male students. Some Candida strains exhibit intrinsic resistance to commonly used antifungal drugs.

Study on the Correlation between Polymorphism of EPAS1 Gene rs1868092 and High Altitude Polycythemia in Tibetan Population

Gao Wenyu, Zeng Rong, Xu Mengna, Zhang Yuanyuan

2023, 8(4):  39-42. 

Asbtract ( 147 )   PDF (3179KB) ( 97 )  

References | Related Articles | Metrics

Objective： To explore the correlation between the polymorphism of rs1868092 in EPAS1 gene and high altitude polycythemia （HAPC） in Tibetan population. Methods： A TaqMan probe was used to examine of the EPAS1 gene SNP rs1868092 locus in 80 Tibetan HAPC patients and 85 healthy Tibetan analysis to investigate the correlation between the SNP rs1868092 locus and the occurrence of HAPC. Results：The frequency of EPAS1 gene rs1868092 genotype GG （11.250% vs. 2.353%， P=0.005， OR=7.375， 95%CI is 1.508-36.066） and allele G（33.125% vs. 16.471%， P<0.001， OR=2.512， 95%CI is 1.490-4.234） was significantly higher than that in the healthy subjects， with statistical significance. Under the dominant model，the frequency of GG+AG genotypes in HAPC patients（P=0.002， OR=2.774， 95%CI is 1.466-5.249） was significantly higher than that in healthy controls， with statistical significance. Conclusion：The G allele of the rs1868092 SNP locus of the EPAS1 gene is a risk factor for Tibetan population to develop HAPC.

Research on the Point Mutation of K-ras Gene in Human Malignant Pleural Mesothelioma

Liu Ruai, Yang Xueqian, Wang Boyong, Mei Wen, Zhou Jinghua, Xiong Wei

2023, 8(4):  43-49. 

Asbtract ( 97 )   PDF (10097KB) ( 57 )  

References | Related Articles | Metrics

Objective：To investigate K-ras gene point mutation in malignant pleural mesothelioma（MPM） tissue of patients from the crocidolite-contaminated area in Dayao Ｃounty， Yunnan Ｐrovince. Methods： Polymerase chain reaction（PCR）and single-strand conformation polymorphism and PCR-DNA sequencing were used to detect the point mutations of K-ras codons 12， 13 and 61 in pleural mesothelium of 59 MPM patients and 13 non-MPM patients. Results： There was no point mutation of K-ras gene in pleural mesothelial tissues of 59 MPM patients and 13 non-MPM patients， and the detection rate of K-ras gene point mutation was 0.0 %. Conclusion： The proportion of K-ras gene mutation type in MPM tissue is relatively low， which suggests that K-ras proto-oncogene point mutation does not play a key role in the induction of MPM in the crocidolite-contaminated area of Dayao county， Yunnan Province.

Correlation between MMP-9 Gene Polymorphism and the Short-Ｔerm Efficacy of TP Regimen in Patients with Ovarian Cancer

Yan Qixing, Zhao Lini, Tang Wenjun, Chen Jinfang, Fang Cao

2023, 8(4):  50-53. 

Asbtract ( 107 )   PDF (3164KB) ( 38 )  

References | Related Articles | Metrics

Objective： To evaluate the relationship between polymorphisms of matrix metalloproteinase 9（MMP-9） rs3918249/rs3918241 and the short-term efficacy of TP regimen in ovarian cancer. Methods： A total of 109 ovarian cancer patients who were pathologically diagnosed and received first-line TP treatment with complete data were included in the study. Blood samples were collected， and DNA was extracted and sequenced by high-throughput sequencing to identify the genotypes of rs3918249 and rs3918241， and their correlation with the efficacy of TP regimen was analyzed. Results： Among the 109 patients， 78 patients had complete or partial response （CR/PR group）， and 31 patients had stable disease or progressive disease（SD/PD group） after treatment. The frequency distribution of rs3918241 allele between the two groups was statistically significant （P<0.05）. The objective response rate was 3.172 times higher （95%CI： 1.064-9.459） in patients carrying the A allele than those carrying the T allele. Conclusion：Advanced ovarian cancer patients carrying rs3918241 A allele have better short-term efficacy when treated with TP regimen.

Analysis of the Effect of Using Low Calcium Dialysate and Standard Calcium Dialysate in Segmented Citrate Anticoagulant Hemodialysis

Zhang Qian, Chang Aying, Yang Zhengchun, Liu Liru

2023, 8(4):  54-59. 

Asbtract ( 130 )   PDF (5236KB) ( 21 )  

References | Related Articles | Metrics

Objective： To analyze the effect of low calcium and standard calcium dialysate segmented citrate anticoagulant hemodialysis in the treatment of uremia patients with high risk of bleeding. Methods：High-risk bleeding patients undergoing hemodialysis at the First Affiliated Hospital of Dali University from January 2021 to January 2022 were enrolled in the study. Low calcium dialysate（control group） and standard calcium dialysate （observation group） were used for segmented citrate anticoagulation. The general data before treatment， the concentration of free calcium ions at the arterial and venous ends at 0.5， 2.0， and 3.0 hours after treatment， the amount of citrate infused at the arterial and venous pot， the coagulation status of the dialyzer and pipeline， and the incidence of adverse events were compared between the two groups. Results： The concentration of free calcium ions in the arterial end and venous end of the observation group was significantly higher than that of the control group at the same time period. The infusion speed of citrate in the arterial end and venous pot was faster， and the duration of dialysis was shorter. The differences had statistical significance（P＜0.05）. The total coagulation rate of the dialysis pipeline （especially the venous pot ） in the observation group was higher than that in the control group， but the incidence of adverse events was significantly lower in the observation group than in the control group， with statistical significance（P＜0.05）. Conclusion： The use of standard calcium dialysate with segmented citrate anticoagulation is safe and associated with fewer adverse events. The venous pot is the main coagulation site， and increasing the amount of citrate infusion and monitoring the calcium level after filtration frequently can ensure smooth completion of hemodialysis in patients with high-risk bleeding.

Application of Temporary Occlusion of Bilateral Internal Iliac Artery Balloon in Cesarean Section of Complete Placenta Previa

Yang Sili, Luo Wanxun, Wang Jianping

2023, 8(4):  60-63. 

Asbtract ( 135 )   PDF (3702KB) ( 41 )  

References | Related Articles | Metrics

Objective： To analyze the safety and effectiveness of bilateral internal iliac artery balloon occlusion in cesarean section of complete placenta previa. Methods： The patients with complete placenta previa diagnosed in No.1 People's Hospital of Dali City from January 2017 to December 2020 were included in the study. Preoperative internal iliac artery balloon occlusion was performed， followed by cesarean section. The intraoperative blood loss， intervention time， neonatal weight， neonatal Apgar score， neonatal asphyxia rate， and hospitalization time were observed and recorded in detail. Results： All patients had central placenta previa， and cesarean section was successfully performed with the assistance of temporary balloon occlusion. One patient had penetrating placenta implantation， and underwent subtotal hysterectomy due to hemostasis difficulties. All newborns survived， and no infections， deep venous thrombosis， iliac artery thrombosis， arterial rupture， organ damage， or maternal deaths occurred postoperatively. Conclusion： For pregnant women suspected of having dangerous complete placenta previa， a detailed treatment plan based on a multidisciplinary emergency team should be developed. Temporary balloon occlusion before cesarean section provides a safe and effective method for treating complete placenta previa with less bleeding and fewer complications while preserving the uterus， improving the patient's postoperative quality of life.

Clinical Analysis of the Therapeutic Effect of Baylor Plaster Immobilization in the Treatment of Legg-Calve-Perthes Disease in Children

Gao Guoxi, Hu Jianhua, Yang Zhao, Zhang Jun, Zhang Chao

2023, 8(4):  64-68. 

Asbtract ( 173 )   PDF (8988KB) ( 36 )  

References | Related Articles | Metrics

Objective： To analyze the clinical efficacy of Baylor plaster immobilization in the treatment of Legg-Calve-Perthes disease in children （hereinafter referred to as "Perthes disease"）. Methods： Before treatment， 18 children with Perthes disease were classified by Catterall. After treatment， all children were followed up for at least 2 years. At the end of the follow-up， Stulberg classification evaluation and Harris score of hip joint were performed. Results： All the children began to receive treatment after the average diagnosis of （2.6±4.1） months， and the average time of Baylor plaster immobilization was （14.3±5.2） months. During the follow-up period， there was no significant limitation on the hip joint activity in the children. At the end of the follow-up， the Stulberg classification results showed that 66.7% were excellent， 22.2% were good， and 11.1% were poor. The follow-up results showed that children under the age of 6 with Catteral type 1 or type 2 had excellent or good Stulberg classification after treatment， while children over the age of 6 with Catteral type 3 or type 4 generally had poor Stulberg classification. However， it was possible to obtain good Stulberg classification results after prolonged treatment with Baylor plaster immobilization. Conclusion： Long-term Baylor plaster immobilization can effectively treat Perthes disease in children.

Clinical Effect of ELOS Technique Combined with Human-Like Collagen Repair Dressing in the Treatment of Patients with Facial Sensitive Skin

Chen Wenqiu, Pang Qin, Zhao Yu, Li Tao, Lin Xianghong, Sun Sujiao

2023, 8(4):  69-72. 

Asbtract ( 204 )   PDF (5008KB) ( 348 )  

References | Related Articles | Metrics

Objective： To evaluate the clinical efficacy of E-max photoelectric radiofrequency therapeutic instrument combined with human-like collagen repair dressing in the treatment of patients with facial sensitive skin. Methods： All enrolled subjects received 4 treatments with E-max photoelectric radiofrequency therapeutic instrument and were simultaneously accompanied by the use of human collagen repair dressing. The erythema distinction value under VISIA skin detector， the score and positive rate of lactic stimulation test， the occurrence of adverse reactions and patients' subjective satisfaction were recorded. Results： After 4 treatments， the erythema distinction value and score of lactate stimulation test were significantly decreased， and the positive rate of lactate stimulation test was reduced from 100.00% before treatment to 20.00%， with statistical significance （P<0.05）. The overall subjective satisfaction rate of patients was 96.67%， indicating a high degree of satisfaction with treatment. No adverse reactions such as pruritus and pigmentation were observed in other patients except for 1 patient with mild skin edema and erythema after treatment. Conclusion： E-max photoelectric radiofrequency therapeutic combined with human-like collagen repair dressing can obviously improve the erythema reaction of sensitive skin， which is worthy of clinical promotion.

Clinical Application of Percutaneous Puncture Biopsy of Peripheral Lung Lesions Guided by Contrast-Enhanced Ultrasound

Zhang Guohui, Cheng Yan, Liang Qiuyue, Yuan Shengxing, Xiao Benlong, Gao Qiuxia, Ding Yunchuan

2023, 8(4):  73-77. 

Asbtract ( 150 )   PDF (6103KB) ( 23 )  

References | Related Articles | Metrics

Objective： To explore the clinical application value of percutaneous puncture biopsy guided by contrast-enhanced ultrasound （CEUS） in peripheral lung lesions. Methods： The medical records of 72 patients with peripheral lung lesions who underwent percutaneous puncture biopsy under ultrasound guidance from January 2017 to August 2021 in Qujing Hospital Affiliated to Kunming Medical University were retrospectively analyzed. According to whether CEUS was performed before biopsy， the patients were divided into CEUS group and conventional ultrasound（CU） group. The necrotic lesion display， number of puncture needles， puncture success rate， initial puncture diagnosis rate， and incidence of complications between the two groups were compared and analyzed. Results：The display rate of necrotic lesion， puncture success rate and first puncture diagnosis rate in CEUS group were significantly higher than those in CU group， and the number of puncture needles was significantly lower than that in CU group. All differences were statistically significant（P<0.05）. Within 48 hours after puncture， one patient in each group had hemoptysis， and there was no statistically significant difference in the incidence of complications（P>0.05）. Conclusion： Percutaneous puncture biopsy guided by contrast-enhanced ultrasound can effectively identify the active tissue in peripheral lung lesions， improve the puncture success rate， and is safe and effective. It is worth promoting in clinical practice.

The Predictive Value of NLR for the Severity of Acute Pancreatitis

Xiao Min, Xiong Miaomiao, Fan Yujuan, Cheng Mingjing, Wang Li, Zhao Weidong

2023, 8(4):  78-82. 

Asbtract ( 111 )   PDF (3697KB) ( 108 )  

References | Related Articles | Metrics

Objective： To investigate the early predictive value of neutrophil-lymphocyte ratio（NLR） in the severity of acute pancreatitis（AP）. Methods： The clinical data of 112 patients with AP admitted to the Department of Gastroenterology， the First Affiliated Hospital of Dali University from January 2020 to January 2022 and 98 healthy subjects during the same period were collected. Patients were divided into non-severe AP groups（n=80） and severe AP groups（n=32） according to the severity of AP. The NLR levels of different crowds were compared. Receiver operating characteristic curve （ROC curve） was used to analyze the diagnostic efficacy of NLR for the severity of AP. Results： The level of NLR in AP patients was significantly higher than that in physical examiner， and the level of NLR in severe AP patients was significantly higher than that in non-severe AP patients， with statistical significance（P<0.01）. The ROC curve analysis showed that NLR had the largest area under the curve （0.761 9） for the diagnosis of AP severity. Conclusion： NLR has a certain value in predicting the severity of AP.

Analysis of Fecal Occult Blood Test in a Second Class A Hospital in Kunming City

Dong Liwen, Li Xi, Li Feiyan, Yi Changqing

2023, 8(4):  83-85. 

Asbtract ( 91 )   PDF (2513KB) ( 68 )  

References | Related Articles | Metrics

Objective： To analyze the common causes of fecal occult blood based on the results of fecal occult blood test in a second class A hospital in Kunming City. Methods： The results of fecal occult blood test using monoclonal antibody method were retrospectively analyzed in 2 000 samples from a second class A hospital in Kunming City from January to February 2021. Results： Among the 2 000 samples， the positive rate of fecal occult blood test was 24.30%， and the positive rate of fecal occult blood test in patients under 50 years old was the highest（43.51%）. The positive rate of fecal occult blood test in patients older than 50 years increased with age （the positive rate of patients aged 80 to 89 decreased slightly）. Conclusion： Digestive hemorrhagic disease， gastrointestinal malignancy and the use of some drugs can lead to positive results in fecal occult blood test. The clinician needs to identify the cause when analyzing positive results of fecal occult blood in patients. For those who are highly suspected of digestive tract bleeding， if the results of fecal occult blood test by monoclonal antibody method are negative， the fecal sample should be further diluted and retested to avoid false negative results and delay disease diagnosis and treatment.

Analysis on the Occurrence and Influencing Factors of Anxiety， Depression and Sleep Disorders in HIV/AIDS Patients Treated with Antiviral Therapy

Zeng Diandian, Jin Yongmei, Tian Bo, Li Chongxi, Liu Jun

2023, 8(4):  86-91. 

Asbtract ( 287 )   PDF (4695KB) ( 97 )  

References | Related Articles | Metrics

Objective：To analyze the occurrence of anxiety， depression and sleep disorders in HIV/AIDS patients treated with antiretroviral therapy and analyze the influencing factors. Methods： Questionnaires were administered to 1 200 HIV/AIDS patients attending Kunming Third People's Hospital from Decembor 2021 to March 2022. The questionnaires used the Hospital Anxiety and Depression Scale and the Pittsburgh Sleep Quality Index to investigate anxiety， depression and sleep disorders in HIV/AIDS patients receiving different antiretroviral treatment regimens. Univariate analysis and multifactorial Logistic regression analysis were used to analyze the factors influencing patients' anxiety， depression and sleep disorders. Results： The prevalence of anxiety， depression， and sleep disorders in 1 200 HIV/AIDS patients was 24.9%， 30.7%， and 39.2%， respectively， and 11.0% had concurrent anxiety， depression， and sleep disorders， and 20.6% had combined anxiety and depression. The results of multifactorial Logistic regression analysis showed that male, having a stable job, treatment time less than 3 months were the protective factors of anxiety, and receiving efavirenz treatment was a risk factor of anxiety; male and having a stable job were the protective factors of depression, and receiving treatment regimens containing non-nucleoside reverse transcriptase inhibitors and protease inhibitors were the risk factors of depression; having a stable job was a protective factor of sleep disorders, and receiving treatment regimens containing non-nucleoside reverse transcriptase inhibitors and protease inhibitors were the risk factors of sleep disorders. Conclusion： The incidence of anxiety， depression and sleep disorders in HIV/AIDS patients is more seriously affected by antiretroviral therapy. The incidence of neuropsychotoxic side effects of integrase inhibitors is lower than that of non-nucleoside reverse transcriptase inhibitors and protease inhibitors， and the monitoring of patients' mental status should be strengthened to optimize the protocol according to patients' conditions.

Construction of the Evaluation Indicators for Independent on-the-Job Training of Medical Personnel in Response to Outbreaks of Infectious Diseases Based on Delphi Method

Zhao Na, Li Haiwen, Wu Yuanyuan, Lu Xiao, Wu Kunli

2023, 8(4):  92-97. 

Asbtract ( 90 )   PDF (4498KB) ( 35 )  

References | Related Articles | Metrics

Objective： To construct the evaluation indicators for independent on-the-job training of medical personnel in response to outbreaks of infectious diseases. Methods： On the basis of the previous research， the target pool of independent entry evaluation indicators of medical personnel for emergent infectious diseases was preliminarily established through literature analysis and subject group discussion. From March to May 2022， Delphi method was used to invite 14 experienced experts in infectious disease prevention and control to conduct two rounds of expert consultation. Using Likert 5-point scale and importance ranking methods， the entries were screened and finally the evaluation indicator system was determined. Results： All questionnaires in both rounds of consultations were collected. The authority coefficients of the first and second rounds of experts were 0.81 and 0.84， respectively. The evaluation indicators for independent on-the-job training of medical personnel in response to outbreaks of infectious diseases were determined after the two rounds of consultations. Conclusion： The Delphi method was used to construct the evaluation indicators of independent entry of medical personnel in the face of infectious diseases， and to provide an evaluation tool for scientific evaluation of medical personnel's ability to work independently in the face of infectious diseases.

Comparison of the Effects of Humidified and Non-Humidified Low-Medium Flow Oxygen Inhalation in Middle-Aged and Elderly People

Song Lipeng, Xu Hong, Zeng Jia, Zhao Guifeng

2023, 8(4):  98-100. 

Asbtract ( 166 )   PDF (2634KB) ( 72 )  

References | Related Articles | Metrics

Objective： To explore whether there is a significant difference in the effect and comfort of oxygen therapy between conventional humidification and non-humidification of low-medium flow oxygen inhalation through nasal oxygen tube for middle-aged and elderly people. Methods： A total of 420 middle-aged and elderly patients receiving long-term low-medium flow oxygen inhalation in the geriatric department of the First Affiliated Hospital of Dali University from July 2020 to February 2021 were selected as the study objects. They were divided into humidified and non-humidified groups by random number table method. Both groups received continuous low-medium flow oxygen inhalation through nasal oxygen tube for more than 12 hours per day. Results： There were no statistically significant differences between the two groups in terms of sputum volume， percutaneous arterial oxygen saturation， sensation of the nasopharynx， and odor sensation of oxygen during oxygen inhalation（P>0.05）. The satisfaction of the two groups was also similar with no statistically significant difference（P>0.05）. Conclusion： Humidification is not necessary in the process of routine low-medium flow oxygen inhalation through nasal oxygen tube for middle-aged and elderly people.