Expression of SOX9 in the Directed Differentiation of Mouse Hepatic Oval Cell into Biliary Epithelial Cells

Abstract

Abstract:

Objective： To investigate the expression changes of SOX9 during the directed differentiation of mouse hepatic oval cell （HOC） into biliary epithelial cell（BEC）. Methods：A mouse HOC activation model was established by 2-acetylaminofluorene gavage and 2/3 partial hepatectomy. HOC were extracted by two-step enzyme digestion and Percoll density gradient centrifugation， and then induced to differentiate into BEC by using epidermal growth factor（EGF）， stem cell growth factor（SCF） and leukemia inhibitory factor（LIF） in vitro. Cell morphological changes before and after differentiation were observed under inverted microscope， BEC were identified by flow cytometry， and the expression of SOX9 before and after differentiation was detected by immunofluorescence.Results： HOC were differentiated into purer BEC up to the 8th generation under the joint action of EGF， SCF and LIF. SOX9 was mainly expressed in the cytoplasm of primary mouse HOC， and gradually entered the nucleus during differentiation， with a significant increase in average light density value. Conclusion： SOX9 enters the nucleus and its expression remarkably increases during differentiation of mouse HOCs into BECs， indicating its possible involvement in the regulation of this differentiation process.

Key words:

"> font-size:10.5pt, ">hepatic oval cell, hepatic progenitor cell, directed differentiation, biliary epithelial cell, SOX9

CLC Number:

Huang Shan, Jin Liquan, Yu Yu, Huang Ji, Zhang Junhua, Chen Yiming.

Expression of SOX9 in the Directed Differentiation of Mouse Hepatic Oval Cell into Biliary Epithelial Cells [J]. Journal of Dali University, 2023, 8(4): 22-27.

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