Effects of Periplaneta americana Extract CⅡ-3 on the Biofilm Formation of Candida albicans

Abstract

Abstract:

Objective： To investigate the effect of Periplaneta americana extract CⅡ-3（hereinafter referred to as "CⅡ-3"） on the formation of Candida albicans biofilm， so as to lay a foundation for the development of antifungal biofilm drugs. Methods： CⅡ-3 was added to Candida albicans solution and incubated with biofilm. CⅡ-3 high dose group， CⅡ-3 low dose group， positive control group and negative control group were set up respectively. The effect of CⅡ-3 on the formation of Candida albicans biofilm was detected by morphological observation and MTT method. The inhibition rate of biofilm was calculated to evaluate the effect of different concentrations of CⅡ-3 on Candida albicans biofilm at different growth stages. Results： Candida albicans could still form biofilm-like structure under the action of CⅡ-3. The inhibition rates of biofilm in high dose group were 25.1% and 13.9%， which were lower than the inhibition rate of fluconazole biofilm（79.3%）. When incubated with Candida albicans biofilm at different growth stages， the highest inhibition rate of CⅡ-3 on 8 h biofilms was 61.7%， followed by the inhibition rates on 16 h and 24 h biofilms （35.4% and 21.9%）， while fluconazole only had significant inhibitory effect on 4 h biofilms （inhibition rate of 75.5%）， and had no significant inhibitory effect on biofilms at other growth stages. The inhibitory effect of CⅡ-3 on Candida albicans aggregation and mature biofilm was 2-9 times that of fluconazole. Conclusion： The extract CⅡ-3 mainly inhibits the biofilm formation of Candida albicans in the aggregation and maturation stages， and the inhibition effect does not show a significant dose-effect relationship.

Key words:

"> font-size:10.5pt, ">Candida albicans, biofilm formation, Periplaneta americana extract CⅡ-3

CLC Number:

Zhao Chunjing, Chen Weiguo, Bai li, Wu Youcong.

Effects of Periplaneta americana Extract CⅡ-3 on the Biofilm Formation of Candida albicans [J]. Journal of Dali University, 2023, 8(4): 28-33.

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