Research on the Point Mutation of K-ras Gene in Human Malignant Pleural Mesothelioma

Abstract

Abstract:

Objective：To investigate K-ras gene point mutation in malignant pleural mesothelioma（MPM） tissue of patients from the crocidolite-contaminated area in Dayao Ｃounty， Yunnan Ｐrovince. Methods： Polymerase chain reaction（PCR）and single-strand conformation polymorphism and PCR-DNA sequencing were used to detect the point mutations of K-ras codons 12， 13 and 61 in pleural mesothelium of 59 MPM patients and 13 non-MPM patients. Results： There was no point mutation of K-ras gene in pleural mesothelial tissues of 59 MPM patients and 13 non-MPM patients， and the detection rate of K-ras gene point mutation was 0.0 %. Conclusion： The proportion of K-ras gene mutation type in MPM tissue is relatively low， which suggests that K-ras proto-oncogene point mutation does not play a key role in the induction of MPM in the crocidolite-contaminated area of Dayao county， Yunnan Province.

Key words:

"> font-size:10.5pt, ">malignant pleural mesothelioma, K-ras gene, point mutation, PCR-SSCP

CLC Number:

Liu Ruai, Yang Xueqian, Wang Boyong, Mei Wen, Zhou Jinghua, Xiong Wei.

Research on the Point Mutation of K-ras Gene in Human Malignant Pleural Mesothelioma [J]. Journal of Dali University, 2023, 8(4): 43-49.

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