Abstract:

Objective： To determine the glycosaminoglycan content in four snail products by 1，9-dimethylmethylene blue spectrophotometry and to verify the detection method. Methods： The 1，9-dimethylmethylene blue spectrophotometry method was used to determine the glycosaminoglycan content in snails， and the specificity， linearity， reproducibility， precision， accuracy， stability and durability of the method were investigated， and the method was used to determine the glycosaminoglycan content in four snail products. Results： The method had good specificity for the determination of glycosaminoglycan content in snails with the maximum absorption wavelength of 520 nm； the contents of glycosaminoglycan in dried white jade snail products， dried glossy large snail products， freeze-dried white jade snail mucus products and freeze-dried glossy large snail mucus products determined by the method at 520 nm were 4.10%， 1.90%， 15.85%， and 9.31%， respectively. Conclusion： After investigation， the method met the relevant requirements for specificity， linearity， repeatability， precision， recovery rate， stability and durability，and it is easy to operate.

Key words:

"> font-size:10.5pt, ">1, 9-dimethylmethylene blue, snail glycosaminoglycan, content determination,  , methodological validation