Abstract: Objective： To explore the medicinal potential of Gentiournoside D， the structural modification of Gentiournoside D was carried out. Methods： Using natural Gentiournoside D as raw material， the fully acetylated Gentiournoside D（1） was obtained by acetylation. Compound 1 was activated by Ｎ，Ｎ'-dicyclohexylcarbodiimide， catalyzed by 4-dimethylaminopyridine， and condensed with amine compounds. Results： Four  Gentiournoside D amide derivatives were efficiently synthesized， namely 2'，3'，4'，6'-tetraacetylated Gentiournoside D formamide（2）， 2'，3'，4'，6'-tetraacetylated Gentiournoside D acetamide（3）， 2'，3'，4'，6'-tetraacetylated Gentiournoside D dimethylamide（4） and 2'，3'，4'，6'-tetraacetylated Gentiournoside D benzylamide（5）.  Compounds 1-5 were new derivatives， and the structures of all derivatives were confirmed by spectroscopic methods. Conclusion： The synthesis method has simple operation， mild reaction conditions， high total yield and good reproducibility.

Key words: font-family:Times New Roman, ">Gentiournoside Dfont-family:Times New Roman, ">；font-family:Times New Roman, "> structural modificationfont-family:Times New Roman, ">；font-family:Times New Roman, "> acetylation productfont-family:Times New Roman, ">；font-family:Times New Roman, "> amidation product