Abstract: Objective： To investigate the chemical constituents and contents of Periplaneta americana extract PA. Methods： The colorimetric method was used to identify the major components in P. americana extract PA. The contents of polypeptides， amino acids， polysaccharides， alkaloids， and phenols in P. americana extract PA were determined by ultraviolet spectrophotometry， while the contents of uracil， inosine， and protocatechuic acid in P. americana extract PA were determined by HPLC. Results： The established methods for the determination of each component had good reproducibility and stability. The main components of 20 batches of P. americana extract PA were polypeptides， amino acids， polysaccharides， alkaloids and phenols， as well as uracil， inosine and protocatechuic acid. There was no significant difference in the main components of different batches of P. americana extract PA. Because of the different harvest time and culture conditions， components of different batches of P. americana extract PA from different breeding plants had certain differences. Conclusion： By analyzing the material composition and content of P. americana extract PA， this study provides a basis for the subsequent screening of functional substances and comprehensive and effective evaluation of the quality of P. americana.

Key words: font-family:Times New Roman, ">Periplaneta americana extractfont-family:Times New Roman, ">；font-family:Times New Roman, "> colorimetric identificationfont-family:Times New Roman, ">；font-family:Times New Roman, "> ultraviolet spectrophotometryfont-family:Times New Roman, ">；font-family:Times New Roman, "> HPLC