Journal of Dali University ›› 2023, Vol. 8 ›› Issue (8): 54-58.

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Effects of High Salt Intervention on Proliferation and Migration of Human Aortic Vascular Smooth Muscle Cell

Deng Wenbin,Huang Shiqiong, Wang Xue,Li Lihua*   

  1. (Department of Geriatrics, The First Affiliated Hospital of Dali University, Dali, Yunnan 671000, China)

  • Received:2022-11-11 Revised:2023-01-20 Online:2023-08-15 Published:2023-07-11

Abstract: Objective: To investigate the effects of different concentrations of Na+ intervention on the proliferation and migration of human aortic vascular smooth muscle cell(HAVSMC). Methods: HAVSMC were cultured as primary cultures and were passaged to the fifth generation. Cells were intervened with different concentrations of Na+(132, 137, 142, 147, 152, 157, 162, and 167 mmol/L), and cell proliferation and migration experiments were conducted at 24, 48, 72, and 96 hours after the intervention. Proliferation activity of HAVSMC was detected by the CCK-8 method, and proliferating cell nuclear antigen(PCNA) expression was analyzed by real-time fluorescence quantitative polymerase chain reaction in order to evaluate proliferation ability of HAVSMC. Additionally, scratch assay was employed to evaluate the migration ability of HAVSMC. Results: Compared with the 132 mmol/L Na+ control group, after 72 hours of cultivation, 137-167 mmol/L Na+ intervention promoted the proliferation ability of HAVSMC. Among all intervention groups(P<0.05), Na+ concentration of 152 mmol/L had a significant pro-proliferation effect(P<0.05) and promoted the migration of HAVSMC. Conclusion: High salt intervention can promote the proliferation and migration of HAVSMC.

Key words: font-family:Times New Roman, ">saltfont-family:Times New Roman, ">;font-family:Times New Roman, "> human aortic vascular smooth muscle cellfont-family:Times New Roman, ">;font-family:Times New Roman, "> proliferationfont-family:Times New Roman, ">;font-family:Times New Roman, "> migration

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