J4 ›› 2012, Vol. 11 ›› Issue (3): 31-34.
目的:提取云龙野生和人工培养两种黑木耳多糖并测定其含量。方法:采用乙醇浸提法提取多糖,紫外分光光度苯酚-硫酸显色法测定粗多糖含量。结果:在8.0~48.0 μg/mL线性范围内,回收率为99.03%,RSD为0.66%(n=6),测到野生和人工培养黑木耳中多糖的收率分别为18.48%和18.29%;野生和人工培养黑木耳中多糖的含量分别为9.21%和8.20%。结论:野生黑木耳比人工培养黑木耳中多糖的含量高,生长环境对黑木耳多糖含量有影响。
Objective: To extract both artificial culture and wild Auricularia auricular polysaccharides and determinate their
contents. Methods: The polysaccharide was extracted with ethanol and the polysaccharide content was wired by UV spectrophotometry phenol -sulfuric acid color method. Results: Within the range of 8.0 -48.0 μg/mL, the recovery ratio of polysaccharide was 99.03% with RDS of 0.66% (n=6). The recovery ratio of polysaccharide in artificial culture and wild Auricularia auricular were 18.48% and 18.29%, respectively. Artificial culture and wild Auricularia auricular in the polysaccharide content was 9.21% and 8.20%, respectively. Conclusion: Wild Auricularia auricular has higher content of polysaccharides than that of culture artificially. Growing environment affects the contents of Auricularia auricula's polysaccharides.