J4 ›› 2012, Vol. 11 ›› Issue (3): 31-34.

• 预防医学 • 上一篇    下一篇

云龙黑木耳中多糖的提取与测定

目的:提取云龙野生和人工培养两种黑木耳多糖并测定其含量。方法:采用乙醇浸提法提取多糖,紫外分光光度苯酚-硫酸显色法测定粗多糖含量。结果:在8.0~48.0 μg/mL线性范围内,回收率为99.03%,RSD为0.66%(n=6),测到野生和人工培养黑木耳中多糖的收率分别为18.48%和18.29%;野生和人工培养黑木耳中多糖的含量分别为9.21%和8.20%。结论:野生黑木耳比人工培养黑木耳中多糖的含量高,生长环境对黑木耳多糖含量有影响。   

  1. 1.大理学院公共卫生学院,云南大理 671000; 2.大理学院基础医学院,云南大理 671000
  • 收稿日期:2011-06-15 修回日期:2011-07-23 出版日期:2012-03-15 发布日期:2012-03-15
  • 作者简介:倪兆林,主要从事流行病与卫生统计学研究.
  • 基金资助:

    大理学院大学生科研基金资助项目(2008DXS131)

Extraction and Determination of Polysaccharides from Yunlong Auricularia auricular

Objective: To extract both artificial culture and wild Auricularia auricular polysaccharides and determinate their
contents. Methods: The polysaccharide was extracted with ethanol and the polysaccharide content was wired by UV spectrophotometry phenol -sulfuric acid color method. Results: Within the range of 8.0 -48.0 μg/mL, the recovery ratio of polysaccharide was 99.03% with RDS of 0.66% (n=6). The recovery ratio of polysaccharide in artificial culture and wild Auricularia auricular were 18.48% and 18.29%, respectively. Artificial culture and wild Auricularia auricular in the polysaccharide content was 9.21% and 8.20%, respectively. Conclusion: Wild Auricularia auricular has higher content of polysaccharides than that of culture artificially. Growing environment affects the contents of Auricularia auricula's polysaccharides.   

  1. 1. Collegle of Public Health, Dali University, Dali, Yunnan 671000, China;
    2. Pre-clinical Collegle, Dali University, Dali, Yunnan 671000, China
  • Received:2011-06-15 Revised:2011-07-23 Online:2012-03-15 Published:2012-03-15

摘要:

目的:提取云龙野生和人工培养两种黑木耳多糖并测定其含量。方法:采用乙醇浸提法提取多糖,紫外分光光度苯酚-硫酸显色法测定粗多糖含量。结果:在8.0~48.0 μg/mL线性范围内,回收率为99.03%,RSD为0.66%(n=6),测到野生和人工培养黑木耳中多糖的收率分别为18.48%和18.29%;野生和人工培养黑木耳中多糖的含量分别为9.21%和8.20%。结论:野生黑木耳比人工培养黑木耳中多糖的含量高,生长环境对黑木耳多糖含量有影响。

关键词: 黑木耳, 乙醇浸提法, 紫外分光光度苯酚-硫酸显色法, 多糖, 含量

Abstract:

Objective: To extract both artificial culture and wild Auricularia auricular polysaccharides and determinate their
contents. Methods: The polysaccharide was extracted with ethanol and the polysaccharide content was wired by UV spectrophotometry phenol -sulfuric acid color method. Results: Within the range of 8.0 -48.0 μg/mL, the recovery ratio of polysaccharide was 99.03% with RDS of 0.66% (n=6). The recovery ratio of polysaccharide in artificial culture and wild Auricularia auricular were 18.48% and 18.29%, respectively. Artificial culture and wild Auricularia auricular in the polysaccharide content was 9.21% and 8.20%, respectively. Conclusion: Wild Auricularia auricular has higher content of polysaccharides than that of culture artificially. Growing environment affects the contents of Auricularia auricula's polysaccharides.

Key words: Auricularia auricula, ethanol extraction, UV spectrophotometry phenol-sulfuric acid method, polysaccharides, content

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