葡萄籽原花青素对下肢动脉硬化闭塞症的效应研究

›› 2017, Vol. 2 ›› Issue (4): 36-40.

葡萄籽原花青素对下肢动脉硬化闭塞症的效应研究

（1.大理大学第一附属医院外六科，云南大理671000；2.大理大学基础医学院，云南大理671000）

收稿日期:2016-04-29 修回日期:2016-12-29 出版日期:2017-04-15 发布日期:2017-04-15
作者简介:陆磊，硕士研究生，主要从事普外基础与临床研究.
基金资助:
云南省教育厅科研基金资助项目（0900158）

Protective Effect of Grape Seed Proanthocyanidin Extract on Lower Extremity Arteriosclerosis Obliterans

（1. Department of General Surgery, The First Affiliated Hospital of Dali University, Dali, Yunnan 671000, China; 2. Pre-clinical
College, Dali University, Dali, Yunnan 671000, China）

Received:2016-04-29 Revised:2016-12-29 Online:2017-04-15 Published:2017-04-15

摘要/Abstract

摘要：

目的：探讨葡萄籽原花青素（GSPE）对下肢动脉硬化闭塞症（LEASO）的防治作用，并初步探讨其作用机制。方法:
新西兰雄性家兔30只，进行LEASO模型复制。随机分为正常对照组（CON组）、模型组（LEASO组）和GSPE干预组（GSPE
组）（0.5 g/kg），每组10 只。于术后第4、8、12 周检测家兔血清TC（总胆固醇）及ox-LDL（氧化低密度脂蛋白）水平，第12
周末检测SOD、MDA含量。分别在术前及术后12周对各组家兔行右后肢Doppler检查；第12周末行家兔双后肢DSA。结
果：与CON 组比较，LEASO 组家兔有跛行，见右后肢足趾甚至右足坏疽，GSPE 组的肢体活动基本正常，未见坏疽；与
LEASO 组比较，GSPE 组血清ox-LDL 水平在第8、12 周均明显降低（P＜0.05）；第12 周末，血清SOD 活性升高的同时MDA
下降（P＜0.05）。Doppler显示GSPE组家兔股浅动脉远端PV值较LEASO组明显升高（P＜0.01）；DSA显示GSPE组家兔右
后肢股浅动脉仅呈节段性闭塞，远端血管显影良好，有侧支循环建立。结论：GSPE对LEASO 具有一定的防治作用，其机
制可能是通过清除自由基、降低脂质过氧化水平等途径实现的。

关键词: GSPE, 下肢动脉硬化闭塞症, 抗氧化, 防治作用

Abstract:

Objective: To investigate the protective effect of Grape Seed Proanthocyanidin Extract（GSPE） on lower extremity
arteriosclerosis obliterans（LEASO）, and to preliminarily explore its action mechanism. Methods: 30 male New Zealand rabbits were
randomly divided into three groups with 10 animals each to duplicate LEASO model. They were normal control group（CON group）,
LEASO model group, and GSPE group（0.5 g/kg）. The TC and ox-LDL in serum were assayed after operation for 4 th, 8 th and 12 th
week. At the end of 12th week, the content of SOD and MDA were detected. The right posterior femoral artery blood flow was detected
by color doppler ultrasound, and digital subtraction angiography（DSA）of the double posterior limbs in rabbit was conducted before
the operation and after 12th week. Results: Compared with CON group, rabbits of LEASO group had claudication and thanatosis was
found in both posterior limbs. GSPE group showed normal activity without thanatosis. Compared with LEASO group, GSPE group's
serum ox-LDL level decreased significantly at the end of 8th,12th week after operation（P＜0.05）. The activity of serum SOD raised
obviously while MDA decreased significantly（P＜0.05）. Compared with LEASO group, GSPE group showed higher PV of right
posterior superficial femoral artery under the detection of color doppler ultrasound （P＜0.01）. Detection of digital subtraction
angiography（DSA）showed that the distal vascular imaging of right posterior superficial femoral artery was good in GSPE group and
visible abundant collateral circulation was established. Conclusion: GSPE had a prominent protective effect on LEASO via removing
free radicals, reducing lipid peroxidation level, etc.

Key words: GSPE, lower extremity arteriosclerosis obliterans, antioxidation, protective effect

中图分类号:

R285.5

陆磊，冯超，崔学兵，杨继武，秦燕，刘仁贵. 葡萄籽原花青素对下肢动脉硬化闭塞症的效应研究[J]. , 2017, 2(4): 36-40.

Lu Lei, Feng Chao, Cui Xuebing, Yang Jiwu, Qin Yan, Liu Rengui. Protective Effect of Grape Seed Proanthocyanidin Extract on Lower Extremity Arteriosclerosis Obliterans[J]. , 2017, 2(4): 36-40.

参考文献

〔1〕BEHAR T，BOSSON J L，GALANAUD J P，et al. Preva?
lence and risk factors of peripheral arterial disease in an
outpatient screening campaign〔J〕. J Mal Vasc，2013，38
（1）：22-28.
〔2〕HUANG H，KOELLE P，FENDLER M，et al. Induction of
inducible nitric oxide synthase（iNOS）expression by ox?
LDL inhibits macrophage derived foam cell migration〔J〕Atherosclerosis，2014，235（1）：213-222.
〔3〕GLIEMANN L，NYBERG M，HELLSTEN Y. Nitric oxide
and reactive oxygen species in limb vascular function：
what is the effect of physical activity〔J〕. Free Radic Res，
2014，48（1）：71-83.
〔4〕TAVAKOLI S，ASMIS R. Reactive oxygen species and thiol
redox signaling in the macrophage biology of atherosclero?
sis〔J〕. Antioxid Redox Signal，2012，17（12）：1785-1795.
〔5〕SUN Y，CHEN X. Ox-LDL-induced LOX-1 expression in
vascular smooth muscle cells：role of reactive oxygen spe?
cies〔J〕. Fundam Clin Pharmacol，2011，25（5）：572-579.
〔6〕孙静，李伟，詹纯烈. 葡萄籽原花青素改善睡眠功能试验
研究〔J〕. 广东医学，2009，30（3）：341-343.
〔7〕ASHA DEVI S，SAGAR CHANDRASEKAR B K，MANJU?
LA K R，et al. Grape seed proanthocyanidin lowers brain
oxidative stress in adult and middle- aged rats〔J〕. Exp
Gerontol，2011，46（11）：958-964.
〔8〕JIA Z，SONG Z，ZHAO Y，et al. Grape seed proanthocyani?
din extract protects human lens epithelial cells from oxida?
tive stress via reducing NF-кB and MAPK protein expres?
sion〔J〕. Mol Vis，2011，17：210-217.
〔9〕FERNÁNDEZ-IGLESIAS A，QUESADA H，DÍAZ S，et al.
Combination of grape seed proanthocyanidin extract and
docosahexaenoic acid-rich oil increases the hepatic detoxi?
fication by GST mediated GSH conjugation in a lipidic post?
prandial state〔J〕. Food Chem，2014，165：14-20.
〔10〕PONS Z，GUERRERO L，MARGALEF M，et al. Effect of
low molecular grape seed proanthocyanidins on blood
pressure and lipid homeostasis in cafeteria diet- fed
rats〔J〕. J Physiol Biochem，2014，70（2）：629-637.

[1]	李永飞，付文鹏，丁路，马增艳，杨永寿. 云南松松针多糖清除自由基及抗脂质过氧化活性研究[J]. , 2017, 2(4): 28-31.
[2]	付文鹏，李永飞，杨秀云，杨柳，杨永寿. 云南松松针中黄酮类化合物抗氧化活性研究[J]. , 2017, 2(4): 32-35.
[3]	张小东，沈怡，刘子琦，徐伟，单华，姜北. 滇西地区29种药用植物抗氧化活性研究[J]. J4, 2016, 15(2): 1-4.
[4]	王万方，张怀令，陈丽，史彩艳，肖怀. 不同加工形式茶叶茶多酚含量及抗氧化活性比较研究[J]. J4, 2014, 13(10): 18-21.
[5]	. 炙升麻与几种升麻属植物抗氧化活性研究[J]. J4, 2012, 11(3): 1-4.
[6]	何丽波, 高海合, 尹正仙, 李花, 何丽仙. 核桃叶抗氧化成分的动态分析[J]. J4, 2012, 11(10): 31-32.