大理大学学报

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基于DNA条形码技术的防风药材市场调查研究

  

  1. (大理大学药学与化学学院,云南大理671000)
  • 收稿日期:2017-06-19 出版日期:2018-02-15 发布日期:2018-02-15
  • 作者简介:罗丹丹,硕士研究生,主要从事中药资源与鉴定研究.
  • 基金资助:

    云南省中青年学术技术带头人后备人才资助项目(2015HB058);国家自然科学基金资助项目(31460084)

Survey on Saposhnikovia divaricata in Herbal Medicine Market Using DNA Barcoding

  1. (College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China)
  • Received:2017-06-19 Online:2018-02-15 Published:2018-02-15

摘要:

目的:对市售防风药材进行调查,为药品检验和市场监督提供参考。方法:采用DNA条形码技术,通过序列比对、变异
位点分析、遗传距离计算和邻接树构建,对市售防风药材进行鉴定。结果:当前市售防风药材来源混杂,包括防风、竹叶防风、
杏叶防风、华山前胡、白花前胡等5种。结论:DNA条形码技术可用于市场防风药材的鉴定,是市场调查和监管的有力手段。

关键词: DNA条形码, 防风, 市场调查, ITS2

Abstract:

Objective: To investigate Saposhnikovia divaricata(Fangfeng)in the herbal medicine market, and to provide references
for the quality control and market supervision. Methods: DNA barcoding was using to study the sequence alignment, analysis of
variable sites, genetic distance calculation, and adjacency tree construction of the Saposhnikovia divaricata(Fangfeng)from the herbal
medicine market. Results: The sources of the Saposhnikovia divaricata(Fangfeng)from the herbal medicine market were complicated,
including: Seseli mairei, Pimpinella candolleana, Peucedanum ledebourielloides, and Peucedanum praeruptorum. Conclusion: DNA
barcoding can be used in Saposhnikovia divaricata(Fangfeng)identification, which is a convenient tool for market investigation and
supervision.

Key words: DNA barcoding, Saposhnikovia divaricata, market investigation, ITS2