J4 ›› 2013, Vol. 12 ›› Issue (4): 48-50.

• 生命科学 • 上一篇    下一篇

洱海水华铜绿微囊藻生长特性的初步研究

  

  1. 1.大理学院公共卫生学院,云南大理 671000;2.大理学院基础医学院,云南大理 671000
  • 收稿日期:2012-09-15 修回日期:2012-10-05 出版日期:2013-04-15 发布日期:2013-04-15
  • 作者简介:倪兆林,硕士研究生,主要从事流行病与卫生统计学研究.
  • 基金资助:

    云南省教育厅科学研究基金资助项目(2012J045);大理学院洱海保护研究专项课题基金资助项目(KYRH201005)

A Study on the Growth and Some Physiological Characteristics of Microcystis aeruginosa Isolated from Erhai Lake

  1. 1. College of Public Health, Dali University, Dali, Yunnan 671000, China;
    2. Pre-clinical College, Dali University, Dali, Yunnan 671000, China
  • Received:2012-09-15 Revised:2012-10-05 Online:2013-04-15 Published:2013-04-15

摘要:

目的:分离纯化出洱海主要的优势铜绿微囊藻并观察其生长规律及特性。方法:采用水滴-涂布平板法分离纯化铜绿微
囊藻,采用90%的丙酮提取叶绿素,酶标仪测定其含量,用显微镜计数藻细胞的个数。结果:铜绿微囊藻细胞密度与OD665呈直线关系,R2=0.993 1,藻细胞的增长速率在第6天达到最大值为0.524,其叶绿素及类胡萝卜素的含量随时间的变化而逐步升高。结论:此铜绿微囊藻的生长符合“S”型曲线,藻细胞密度与吸光度成线性相关,其生长速率在第6天达到最大。

关键词: 洱海, 铜绿微囊藻, 分离, 生长曲线, 叶绿素a

Abstract:

Objective: To study on the growth law and some physiological characteristics of Microcystis aeruginosa isolated from Erhai lake. Methods: Purified Microcystis aeruginosa was separated with water droplets method and the spread plate method. Microscopic was used to count the density of algal cells;chlorophyll and carotenoid were extracted with 90% acetone and ELIASA determination of its content. Results: Microcystis aeruginosa cells density was in a linear relationship with OD665, R2 =0.993 1;algal cell growth rate in sixth days reached a maximum of 0.524;chlorophyll and carotenoid content increased gradually over time. Conclusion: This Microcystis aeruginosa growth is in line with the“S”shaped curve;cell density and absorbance is linearly related and the growth rate of the maximum on the sixth day.

Key words: Erhai lake, Microcystis aeruginosa, isolate, growth curve, chlorophyll a

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