Abstract:

 Objective:To establish a high performance liquid chromatography（HPLC）method for the simultaneous determination of berberine hydrochloride and baicalin in Mingmushangqing tablets. Methods: HPLC was carried out with indexes of Agilent Zorbax Exlipse XDB-C18（4.6 mm×250 mm, 5 μm）, column temperature at 25 ^oC, the mobile phase of methanol-0.2 % phosphoric acid（55:45）, flow rate at 1.0 mL·min^-1, and detection wavelength at 265 nm. Results：The method showed good linear relationships within the range of 1.02-20.40 μg·mL^-1 （r =0.999 9）for berberine hydrochloride and 4.54-90.80 μg·mL^-1 （r =0.999 8）for baicalin,respectively. The average recoveries were 99.2%（Relative Standard Deviation RSD=1.1%）and 101.2%（RSD=1.6%）. Conclusion:The method described above is simple, accurate and reproducible which could be used for the quality control of Mingmushangqing tablets.

Key words: HPLC, Mingmushangqing tablets, berberine hydrochloride, baicalin