J4 ›› 2012, Vol. 11 ›› Issue (9): 95-97.

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Determination of Chlorogenic Acid in Sangjiangganmao Capsules by RP-HPLC

Objective: To establish a RP-HPLC method for the determination of chlorogenic acid in Sangjiangganmao capsules.
Methods: An Agela XBP-C18 (250 mm×4.6 mm, 5 μm) column was applied, the mobile phase was acetonitrile-0.4% phosphoric （9:91）, the detection wavelength was 327 nm with the flow rate of 0.1mL/min. Results: There was a good linear relationship in the chlorogenic acid concentration range of 0.02-0.25 μg (r=0.999 8). The average recovery was 99.5%, RSD=0.67% (n=6). Conclusion: The method was with good repeatability, flexibility and high sensitivity, which can be used in the quality control of Sangjiangganmao capsules.   

  1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China
  • Received:2012-04-10 Revised:2012-05-05 Online:2012-09-15 Published:2012-09-15

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Abstract:

Objective: To establish a RP-HPLC method for the determination of chlorogenic acid in Sangjiangganmao capsules.
Methods: An Agela XBP-C18 (250 mm×4.6 mm, 5 μm) column was applied, the mobile phase was acetonitrile-0.4% phosphoric （9:91）, the detection wavelength was 327 nm with the flow rate of 0.1mL/min. Results: There was a good linear relationship in the chlorogenic acid concentration range of 0.02-0.25 μg (r=0.999 8). The average recovery was 99.5%, RSD=0.67% (n=6). Conclusion: The method was with good repeatability, flexibility and high sensitivity, which can be used in the quality control of Sangjiangganmao capsules.

Key words: RP-HPLC, Sangjiangganmao capsules, chlorogenic acid

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