J4 ›› 2013, Vol. 12 ›› Issue (6): 38-42.

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Comparison of Three Methods of Listeria monocytogenes Detection

  

  1. Disease Control and Prevention Center of Kunming, Kunming 650228, China
  • Received:2012-09-16 Revised:2012-10-16 Online:2013-06-15 Published:2013-06-15

Abstract:

Objective: To find a suitable method for rapid detection of Listeria monocytogenes (LM)in grassroots labs. Methods:
GB law, real-time PCR and LAMP were adopted to detect LM, then, the results were evaluated. Results: The results of the three mothods were quite consistent. The detection process of GB law took 5 to 7 days. While the real -time PCR requiring high experimental conditions and cost took 2.5 days. LAMP method, requiring low experimental conditions and cost, took the same time as the real-time PCR. Conclusion: The LAMP assay was a sensitive, specific, rapid and economical method for the detection of LM, which could be widely used by grassroots labs.

Key words: GB law, LAMP, PCR, detection of Listeria monocytogenes

CLC Number: